A New Fungal Endophyte, Scolecobasidium humicola, Promotes Tomato Growth under Organic Nitrogen Conditions

Mahmoud, Rola; Narisawa, Kazuhiko

doi:10.1371/journal.pone.0078746

Cited by 68 publications

(42 citation statements)

References 23 publications

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“…Several DSE fungi have been used against fungal diseases such as rice blast disease caused by Magnaporthe oryzae, cabbage Verticillium wilt caused by Verticillium longisporum, and tomato wilt caused by Verticillium dahliae (Narisawa et al, 2004;Andrade-Linares et al, 2011;Su et al, 2013). Moreover, DSE fungi have been reported as plant growth promoters acting through enhancing nutrient acquisition of vegetable crops such as tomato, clover, and asparagus (Mahmoud and Narisawa, 2013;Della-Monica et al, 2015;Surono and Narisawa, 2017). However, these previous studies focused mainly on vegetable crops, and no studies have examined the potential of DSE fungi on fruit crops.…”

Section: Introductionmentioning

confidence: 99%

The Effectiveness of a Dark Septate Endophytic Fungus, Cladophialophora chaetospira SK51, to Mitigate Strawberry Fusarium Wilt Disease and With Growth Promotion Activities

et al. 2020

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Strawberry Fusarium wilt, caused by the virulent fungus Fusarium oxysporum formae speciales fragariae (Fof) is a devastating soil-borne disease that causes severe production losses worldwide, including Japan. Fof is one of the top 10 fungal pathogens that threaten global crop security, and a method to effectively control this pathogen has yet to be found. This study aimed to investigate the effectiveness of dark septate endophytic (DSE) fungi against Fof to develop an efficient, effective, and environmentally friendly approach to improve plant health and fitness. A total of 19 fungal isolates were assessed, out of which three (SK47, SK48, and SK51) were selected based on their effectiveness in disease suppression in controlled growth chamber conditions using a soil system. Isolates SK47, SK48, and SK51 suppressed disease severity by 85.71, 61.90, and 90.48%, respectively. Molecular identification based on highly conserved small subunit (SSU), internal transcribed spacer (ITS), and large subunit (LSU) nrRNA regions identified these isolates as DSE Exophiala sp., Exophiala pisciphila, and Cladophialophora chaetospira, respectively. The sequences were deposited under accession numbers MN811693-MN811695 in the GenBank database. Notably, our results revealed that isolate C. chaetospira SK51 possessed superior growth promotion activities as well as disease suppression by significantly increased plant growth parameters (shoot and root dry mass, chlorophyll content, flower bud initiation, and number of fruit) in comparison to control plants and other two fungal candidates. Root colonization by C. chaetospira SK51 was visualized, and it was confirmed that the symbiosis with strawberry plants occurred successfully. Our results provide new insights in the application of DSE fungus C. chaetospira SK51 as a biocontrol agent on

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Section: Introductionmentioning

confidence: 99%

The Effectiveness of a Dark Septate Endophytic Fungus, Cladophialophora chaetospira SK51, to Mitigate Strawberry Fusarium Wilt Disease and With Growth Promotion Activities

et al. 2020

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“…Although some studies showed that DSEs can have negative effects on their host (Terhonen et al 2016;Wilcox and Wang 1987;Tellenbach et al 2011;Reininger et al 2012), a meta-analysis focusing on controlled experiments showed that DSEs generally had positive effects on plants (Newsham 2011). DSEs positively affected different parameters of tomato plants and fruit quality in greenhouse conditions (Andrade-Linares et al 2011b) and enhanced tomato plant growth when an organic N source was present (Mahmoud and Narisawa 2013). DSE inoculation also helped pine seedlings absorb more P and N .…”

Section: Introductionmentioning

confidence: 99%

Impact of dark septate endophytes on tomato growth and nutrient uptake

Yakti

Kovács

Vági

et al. 2018

Plant Ecology & Diversity

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Background: Dark septate endophytes (DSEs) represent a form-group of ascomycetous fungi that inhabit the roots of a wide range of plant species, but our knowledge on their interaction with the host plants is still limited. Aims: This study was conducted to examine the effect of DSEs on the nutrition and growth of tomato (Solanum lycopersicum) in order to assess their potential application in horticultural plant production. Methods: The capacity of two model DSE species, Periconia macrospinosa and Cadophora sp. to mobilise different forms of N and P (organic and inorganic) was analysed, and an in vitro bio assay with tomato plantlets was applied to screen the compatibility of these fungi with the plant. Pot-culture experiments with and without compartments were conducted to study the effects of these DSEs on the growth and nutrient uptake of tomato plants grown with organic and inorganic N and P sources. Results: Periconia macrospinosa, but not Cadophora sp., increased the root and shoot biomass of tomato plants when organic nutrient resources were present, and both DSEs promoted shoot growth when cultivated with inorganic fertilisers. Analysis of N and P concentrations indicated that the growth response of tomato with inorganic fertilisation was not based on DSE-improved plant nutrition. However, P. macrospinosa improved N uptake from organic sources. Conclusion: The positive effects of DSEs seem to be due to nutrient mobilisation rather than to hyphal transport to the plant.

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“…Several studies have reported the potential of DSE fungi in agriculture. Mahmoud and Narisawa (2013) reported that Scolecobasidium humicola plays a role in increasing the growth of tomato plants in abiotic stress. Tellenbach et al (2013) reported that Phialocephala europaea could inhibit the growth of Phytophthora citricola.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Capability of Dark Septate Endophyte Against Mancozeb Fungicide

Manalu

Soekarno

Tondok

et al. 2020

JIPI

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Mancozeb is a group member of dithiocarbamat fungicides that most widely applied in the field. The use of mancozeb causes residues that have negative effects on agricultural production system and on agricultural environment. This research aimed to obtain dark septate endophyte (DSE) fungal isolates that are tolerant to mancozeb fungicide. The study was consisted of three steps, i.e., the isolation of DSE fungi from chili roots, the effect of DSE fungal isolates on chili seeds, and the growth ability of DSE isolates on medium supplemented with mancozeb. Thirteen DSE fungal isolates were isolated from chilli pepper roots and grown in the field assumed to be contaminated by pesticides. The isolates of DSE fungi caused the growth of chili seeds were normal and abnormal. Seven DSE isolates were able to grow well at PDA medium suplemented with mancozeb at concentrations of 100, 200, 400, and 800 ppm with different levels of relative growth inhibition. Mancozeb on PDA medium influenced the mycelial growth and colony color of DSE fungal isolates. The biomass of DSE fungal isolates were reduced when they were grown at malt extract broth (MEB) medium suplemented various levels of mancozeb. ABSTRAKMankozeb adalah kelompok fungisida ditiokarbamat yang paling banyak digunakan di lapangan. Penggunaan mankozeb yang tidak sesuai dapat menyebabkan dampak negatif pada sistem produksi dan lingkungan pertanian. Penelitian ini bertujuan untuk memeroleh isolat cendawan Dark Septate Endophyte (DSE) yang toleran terhadap fungisida berbahan aktif mankozeb. Penelitian terdiri atas tiga tahap, yaitu isolasi cendawan DSE dari akar tanaman cabai, pertumbuhan isolat cendawan DSE pada tanaman cabai, dan kemampuan tumbuh cendawan DSE pada media yang mengandung mankozeb. Berdasarkan hasil penelitian diperoleh 13 isolat cendawan DSE dari akar tanaman sawit yang terkontaminasi oleh penggunaan pestisida. Isolat cendawan DSE yang diperoleh menyebabkan pertumbuhan benih cabai normal dan tidak normal. Tujuh isolat cendawan DSE mampu tumbuh dengan baik pada media PDA yang telah diberi penambahan konsentrasi mankozeb, yaitu 100, 200, 400, dan 800 ppm dengan hambatan pertumbuhan relatif yang berbeda. Penambahan mankozeb pada media PDA memengaruhi pertumbuhan miselium dan warna koloni isolat cendawan DSE. Pertumbuhan cendawan DSE pada media cair Malt Extract (ME) yang ditambahkan konsentrasi mankozeb menyebabkan penurunan biomassa cendawan DSE. Kata kunci: ditiokarbamat, hambatan pertumbuhan relatif, toleran

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A New Fungal Endophyte, Scolecobasidium humicola, Promotes Tomato Growth under Organic Nitrogen Conditions

Cited by 68 publications

References 23 publications

The Effectiveness of a Dark Septate Endophytic Fungus, Cladophialophora chaetospira SK51, to Mitigate Strawberry Fusarium Wilt Disease and With Growth Promotion Activities

The Effectiveness of a Dark Septate Endophytic Fungus, Cladophialophora chaetospira SK51, to Mitigate Strawberry Fusarium Wilt Disease and With Growth Promotion Activities

Impact of dark septate endophytes on tomato growth and nutrient uptake

Isolation and Capability of Dark Septate Endophyte Against Mancozeb Fungicide

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