A new Genotype of Japanese Encephalitis Virus from Indonesia

Chen, Woan-Ru; Rico-Hesse, Rebeca; Tesh, Robert B.

doi:10.4269/ajtmh.1992.47.61

Cited by 132 publications

(115 citation statements)

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“…Primers were used to amplify the prM gene, and an overlapping region of the NS5-3Ј UTR of the genome. 5 These regions of the genome were chosen for analysis to: 1) identify the genotype; 8,9 2) compare isolates of JE virus from PNG with others from the Torres Strait; and 3) determine whether PNG isolates of JE had the unique eleven base deletion in the NS5-3Ј UTR as observed in isolates from the Torres Strait. 5 cDNA was obtained by reverse transcription of 2 L of the total RNA using the antisense primer of each primer pair.…”

Section: Methodsmentioning

confidence: 99%

“…Genotype I is found primarily in Laos, northern Thailand, Cambodia, and Malaysia; Genotype II consists of isolates from southern Thailand, Malaysia, and Indonesia; Genotype III appears to be the most widely distributed and includes isolates from Japan, Korea, China, Malaysia, Philippines, Indonesia, India, and Sri Lanka; and Genotype IV appears to be restricted to Indonesia. [8][9][10] Comparison of the genetic sequence of the 1995 isolates of JE virus in the prM region revealed they probably had a common source and were most closely related to a 1970 isolate from Malaysia and a 1981 isolate from Indonesia, both of which belonged to Genotype II. 5 Sequence comparison of an overlapping region of the non-structural and 3Ј untranslated region (NS5-3Ј UTR) confirmed the relationship between the Australian, Indonesian, and Malaysian isolates, and also revealed the Australian isolates had a unique eleven base deletion immediately following the stop codon in the 3Ј untranslated region.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of Japanese encephalitis virus from mosquitoes (Diptera: Culicidae) collected in the Western Province of Papua New Guinea, 1997-1998.

Johansen¹,

Hurk²,

Ritchie³

et al. 2000

Am J Trop Med Hyg

104

109

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After Japanese encephalitis (JE) virus emerged in the Torres Strait in Australia in 1995, investigations were initiated into the origin of the incursion. New Guinea was considered the most likely source, given its proximity to islands of the Torres Strait. Almost 400,000 adult mosquitoes were processed for virus isolation from 26 locations in the Western Province of Papua New Guinea (PNG) between February 1996 and February 1998, yielding three isolates of JE virus. Two isolates of Murray Valley encephalitis, 17 isolates of Sindbis, and 1 each of Sepik and Ross River viruses were also obtained. Nucleic acid sequences of the PNG JE isolates were determined in the prM region, and in a region overlapping a part of the fifth nonstructural protein and the 3' untranslated region. The PNG isolates belonged to genotype II, and shared > 99.2% identity with isolates from humans and mosquitoes from the Torres Strait, suggesting that PNG is the source of incursions of JE virus into Australia.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation of Japanese encephalitis virus from mosquitoes (Diptera: Culicidae) collected in the Western Province of Papua New Guinea, 1997-1998.

Johansen¹,

Hurk²,

Ritchie³

et al. 2000

Am J Trop Med Hyg

104

109

View full text Add to dashboard Cite

show abstract

“…By pair-wise sequence comparison of 67 nucleotide sequences, a dendrogram of the virus relationship was constructed with the aid of the "Cluster Analysis" computer program (48). The prM nucleotide sequence information of 56 JE strains was obtained from previous publications (9,10). The nucleotide sequence data of the E protein genes of Nakayama, Beijing-1, Kamiyama, 691004 and Muar were obtained from Hasegawa et al (18) and ThCMAr4492 and ThCMAr6793 from our previous report (1).…”

Section: Two Primers Je317smentioning

confidence: 99%

“…Critical B-cell epitopes of the E protein produce neutralizing antibodies that are either type-specific, cross-reacting to the same antigenic complex, or common to all flaviviruses (22 (4,26). Recently, Chen et al (9,10) identified four genotypes of JE virus by analyzing 240 nucleotides from the prM gene region, and tried to explain the transmission, evolution, epidemiology and possible pathogenesis of JE virus.…”

mentioning

confidence: 99%

Antigenic and Genetic Variations among Japanese Encephalitis Virus Strains Belonging to Genotype 1

Ali

Igarashi

1997

Microbiology and Immunology

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Hyperimmune antisera against four Japanese encephalitis (JE) virus strains, ThCMAr4492 and ThCMAr6793 from Thailand and Nakayama and JaGAr01 from Japan, were used to analyze the antigenic relationships among 12 Thai strains belonging to genotype 1, and two Japanese strains and one Chinese strain belonging to genotype 3. The antiserum for ThCMAr6793 significantly neutralized nine of the 12 Thai strains, none of which was significantly neutralized by antisera for the Nakayama and JaGAr01 strains. The antiserum for ThCMAr4492 neutralized only its homologous strain; therefore, ThCMAr4492 was antigenically different from all other strains. Two Thai strains (Subin and KE-093/83) were significantly less neutralized by all four of the antisera tested. In the deduced amino-acid sequence of the E protein, the 12 Thai strains revealed 100 to 98.2 % identity among them and 90.0 to 98.8 % identity with the published strains, respectively. Among significant amino-acid substitutions, three residues at positions E-222, E-327 and E-366 were shared by all of the Thai strains, whereas residues at E-89, E-123, E-131, E-178, E-293, E-351 and E-373 seemed to be strain-specific. The amino acids at positions E-178, E-327, E-351, E-373 and E-366 are found either in the peptides with functional T-helper cell epitopes or in the ectodomain of the E protein of other flaviviruses. These amino acids may therefore be responsible for determining the antigenic heterogeneity of these strains.

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“…Geographic-specific viral genotypes or topotypes have since been identified for dengue virus types 1 and 2 [2,28], Japanese encephalitis virus [7], hepatitis C virus [31], poliovirus type 1 [29], hepatitis A virus [30], Ross River virus [20], rabies virus [32], vesicular stomatitis virus [25], hmnan T-cell lymphotropic virus type I [23, 40,42], and human papillomavirus type 16 [6,17]. Further analyses of peromyscine rodent-borne hantaviruses from the eastern United States will be required to validate the apparent geographic-specific genetic and phylogenetic segregation of Monongahela-1 from strains of FC virus from the western United States and Canada.…”

mentioning

confidence: 99%

Genetic and phylogenetic analyses of hantaviral sequences amplified from archival tissues of deer mice (Peromyscus maniculatus nubiterrae) captured in the eastern United States

Song

Baek

Nagle

et al. 1996

Archives of Virology

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Summary. The S and M segments of a hantavirus, enzymatically amplified from tissues of Cloudland deer mice (Peromyscus maniculatus nubiterrae) captured during 1985 in West Virginia, diverged from strains of Four Corners virus from the southwestern United States bymore than 16% and 6% at the nucleotide and amino acid levels, respectively. Phylogenetic analysis suggested that this virus strain (designated Monongahela) forms a possible evolutionary link between the Four Corners and New York hantaviruses.

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A new Genotype of Japanese Encephalitis Virus from Indonesia

Cited by 132 publications

References 0 publications

Isolation of Japanese encephalitis virus from mosquitoes (Diptera: Culicidae) collected in the Western Province of Papua New Guinea, 1997-1998.

Isolation of Japanese encephalitis virus from mosquitoes (Diptera: Culicidae) collected in the Western Province of Papua New Guinea, 1997-1998.

Antigenic and Genetic Variations among Japanese Encephalitis Virus Strains Belonging to Genotype 1

Genetic and phylogenetic analyses of hantaviral sequences amplified from archival tissues of deer mice (Peromyscus maniculatus nubiterrae) captured in the eastern United States

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