A new high-performance liquid chromatographic method for the quality control of bioconverted Mori Folium extracts with appropriate marker compounds related to antidiabetes

Abstract

A HPLC method was developed to assess the enhanced bioactivity of bioconverted Mori Folium (MF) extract and to apply a quality-control system. Liquid-liquid extraction was applied to observe changes in chemical composition through enzymatic bioconversion. The ethyl acetate layer was used for quality control of the anti-diabetic functional MF product. A high-performance liquid chromatography method was developed to analyze five marker compounds (syringaldehyde, trans-caffeic acid, morin 3-O-β-D-glucopyranoside, astragalin, and moracin M 3′-O-β-glucopyranoside) within 60 min. Optimized sample preparation was sonication for 44 min and a water-to-material ratio of 102.5 mL/g by response surface methodology. Comparing MF water extract, trans-caffeic acid and syringaldehyde contents were increased 1.89 times and 2.70 times at Viscozyme-bioconverted material by applying the developed method. This HPLC method can be used to control the quality of bioconverted MF based on suitable bioactive markers and also applied to develop anti-diabetic functional foods containing Mori Folium.

In Vitro Anti-Diabetic, Anti-Inflammatory, Antioxidant Activities and Toxicological Study of Optimized Psychotria malayana Jack Leaves Extract

“…Moracin M-3 -O-β-D-glucopyranoside was extracted from the leaves of Morus insignis and exhibited a significant hypoglycemic effect in streptozotocin-induced hyperglycemic rats [72]. The selection of moracin M-3 -O-β-D-glucopyranoside as a promising agent was based on its established anti-diabetic activities [73]. In addition, it demonstrates significant potential in the therapeutic treatment of inflammatory illnesses.…”

Purification, structural characterization, and neuroprotective effect of 3,6‐diisobutyl‐2,5‐piperazinedione from Halomonas pacifica CARE‐V15 against okadaic acid‐induced neurotoxicity in zebrafish model

“…The drying gas flow rate was 15 L/min, the nebulization gas flow rate was 3 L/min, the desolation line temperature was 250°C, and the heating block temperature was 400°C at a flow rate of 0.7 mL/min. [28]…”

Bifunctional free radical photoinitiator based on syringaldehyde

“…Syringaldehyde is abundantly present in lignocellulosic (Mori Folium, Apricot pits, Gastrochilus bellinus , etc. ) 31–35 and has wide applications in biomedicine (monitoring of disease‐related markers, modification activity of antimicrobial agents, etc.) 36–39 .…”

“…30 Syringaldehyde is abundantly present in lignocellulosic (Mori Folium, Apricot pits, Gastrochilus bellinus, etc.) [31][32][33][34][35] and has wide applications in biomedicine (monitoring of disease-related markers, modification activity of antimicrobial agents, etc.). [36][37][38][39] In this study, syringaldehyde was chosen as the raw material for the design and synthesis of S1 (Scheme 1).…”