A New Highly Hydrophilic Electrochemical Implant Titanium Surface

Trisi, Paolo; Berardini, Marco; Falco, Alessandra; Sandrini, Enrico; Vulpiani, Michele Podaliri

doi:10.1097/id.0000000000000605

Cited by 10 publications

(7 citation statements)

References 39 publications

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“…Mechanistically, the success of the therapy using these biomaterials can be evaluated by the profile of osteoin-tegration promoted by the osteoprogenitor cells in response to them. It is known that the physico-chemical properties of the biomaterial surfaces decisively trigger biological phenomena able to modulate cellular response and later impact the time and quality of osseointegration [2][3][4]. Therefore, a special look at cellular transducers that govern cell-biomaterial response becomes necessary.…”

Section: Introductionmentioning

confidence: 99%

Nano hydroxyapatite-blasted titanium surface creates a biointerface able to govern Src-dependent osteoblast metabolism as prerequisite to ECM remodeling

Fernandes

Bezerra

Ferreira

et al. 2018

Colloids and Surfaces B: Biointerfaces

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Over the last several years, we have focused on the importance of intracellular signaling pathways in dynamically governing the biointerface between pre-osteoblast and surface of biomaterial. Thus, this study investigates the molecular hallmarks involved in the pre-osteoblast relationship with different topography considering Machined (Mc), Dual Acid-Etching (DAE), and nano hydroxyapatite-blasted (nHA) groups. There was substantial differences in topography of titanium surface, considering Atomic Force Microscopy and water contact angle (Mc = 81.41 ± 0.01; DAE = 97.18 ± 0.01; nHA = 40.95 ± 0.02). Later, to investigate their topography differences on biological responses, pre-osteoblast was seeded on the different surfaces and biological samples were collected after 24 h (to consider adhesion signaling) and 10 days (to consider differentiation signaling). Preliminary results evidenced significant differences in morphological changes of pre-osteoblasts mainly resulting from the interaction with the DAE and nHA, distinguishing cellular adaptation. These results pushed us to analyze activation of specific genes by exploring qPCR technology. In sequence, we showed that Src performs crucial roles during cell adhesion and later differentiation of the pre-osteoblast in relationship with titanium-based biomaterials, as our results confirmed strong feedback of the Src activity on the integrin-based pathway, because integrin-ß1 (∼5-fold changes), FAK (∼12-fold changes), and Src (∼3.5-fold changes) were significantly up-expressed when Src was chemically inhibited by PP1 (5 μM). Moreover, ECM-related genes were rigorously reprogrammed in response to the different surfaces, resulting on Matrix Metalloproteinase (MMP) activities concomitant to a significant decrease of MMP inhibitors. In parallel, we showed PP1-based Src inhibition promotes a significant increase of MMP activity. Taking all our results into account, we showed for the first time nano hydroxyapatite-blasted titanium surface creates a biointerface able to govern Src-dependent osteoblast metabolism as pre-requisite to ECM remodeling.

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Section: Introductionmentioning

confidence: 99%

Nano hydroxyapatite-blasted titanium surface creates a biointerface able to govern Src-dependent osteoblast metabolism as prerequisite to ECM remodeling

Fernandes

Bezerra

Ferreira

et al. 2018

Colloids and Surfaces B: Biointerfaces

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“…Active new bone formation was observed around the Ti disk in the S-100 group compared with that in the Control group. To acquire sufficient osseointegration of implant surface, appropriate bone quality, fixture microstructure, and bone bioactivity are important [22][23][24][25]. In this study, mirror-polished titanium discs were placed in insufficient areas of cancellous bone.…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Evaluation of Titanium Surface Modification for Biological Aging by Electrolytic Reducing Ionic Water

et al. 2019

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In this study, using electrolytic reducing ionic water (S-100®), a novel surface treatment method safely and easily modifying the surface properties was evaluated in vitro and in vivo. Ti-6Al-4V disks were washed and the disks were kept standing on a clean bench for one and four weeks for aging. These disks were immersed in S-100® (S-100 group), immersed in ultra-pure water (Control group), or irradiated with ultraviolet light (UV group), and surface analysis, cell experiment, and animal experiment were performed using these disks. The titanium surface became hydrophilic in the S-100 group and the amount of protein adsorption and cell adhesion rate were improved in vitro. In vivo, new bone formation was noted around the disk. These findings suggested that surface treatment with S-100® adds bioactivity to the biologically aged titanium surface. We are planning to further investigate it and accumulate evidence for clinical application.

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“…Exposure of Ti to simulated physiological solutions (i.e. Ringer's solution and saline, which contains calcium and phosphate ions) leads to adsorption of calcium phosphate [19] Photosensitizers [19] Bleaching agents [19] Cold plasma [19] Resin cement [19] Angiogenesis [19] Proliferation and re-epithelialization of cells [19] Vascular Endothelial Growth Factor (VEGF) induced cell migration [19] Peri-implant inflammation [20] Cell death [19] Carcinogenesis & mutagenesis [19] Mitochondrial dysfunction [19] [ 19,20] Calcium NaOH reagent [20] Physiological solution (Ringer's solution and saline) [15] Improved bone contact and results in good osseointegration [22] Inhibit apatite formation [21] [15, 21,22] Phosphorus Anodization in phosphoric acid solution [11] Calcium phosphate coating [24] Biological residue (oral cavity) [25] Modulate cytokines production [23] Promote stem cell differentiation [24] Increase osteoblasts production [24] Increase bone formation [24] White residue obstructing Ti implant [25] [2, 11,[23][24][25] Phosphorus Anodization in phosphoric acid solution [11] Calcium phosphate coating [24] Biological residue (oral cavity) [25] Modulate cytokines production [23] Promote stem cell differentiation [24] Increase osteoblasts production [24] Increase bone formation…”

Section: Calcium (Ca)mentioning

confidence: 99%

“…Furthermore, dental implant surfaces treated with 37% phosphoric acid modulates cytokine production by blood mononuclear cells, establishing a balance between proteins with anti and pro-inflammatory activity, thus promoting the success of dental implants [23]. A Ti surface coating based on calcium phosphate showed high hydrophilicity and high osseointegration, promoting stem cell differentiation, increasing osteoblast production and bone formation, thus resulting in increasing bone formation in a shorter time [24].…”

Section: Phosphorus (P)mentioning

confidence: 99%

Contamination of titanium dental implants: a narrative review

et al. 2020

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Contamination of titanium dental implants may lead to implant failure. There are two major types of contaminants: the inorganic and organic contaminants. The inorganic contaminants mostly consist of elements such as calcium, phosphorus, chlorine, sulphur, sodium, silicon, fluorine and some organic carbons. Whereas organic contaminants consist of hydrocarbon, carboxylates, salts of organic acids, nitrogen from ammonium and bacterial cells/byproducts. Contaminants can alter the surface energy, chemical purity, thickness and composition of the oxide layer, however, we lack clinical evidence that contaminations have any effect at all. However, surface cleanliness seems to be essential for implant osseointegration.These contaminants may cause dental implants to fail in its function to restore missing teeth and also cause a financial burden to the patient and the health care services to invest in decontamination methods. Therefore, it is important to discuss the aetiology of dental implant failures. In this narrative review, we discuss two major types of contaminants: the inorganic and organic contaminants including bacterial contaminants. This review also aims to discuss the potential effect of contamination on Ti dental implants.

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A New Highly Hydrophilic Electrochemical Implant Titanium Surface

Cited by 10 publications

References 39 publications

Nano hydroxyapatite-blasted titanium surface creates a biointerface able to govern Src-dependent osteoblast metabolism as prerequisite to ECM remodeling

Nano hydroxyapatite-blasted titanium surface creates a biointerface able to govern Src-dependent osteoblast metabolism as prerequisite to ECM remodeling

In Vitro and In Vivo Evaluation of Titanium Surface Modification for Biological Aging by Electrolytic Reducing Ionic Water

Contamination of titanium dental implants: a narrative review

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