A new hyperelastic lookup table for RT-DC

Abstract

Real-time deformability cytometry (RT-DC) is an established method that quantifies features like size, shape, and stiffness for whole cell populations on a single-cell level in real time. To extract the...

Shear rheology of methyl cellulose based solutions for cell mechanical measurements at high shear rates

“…That this is a valid approach was recently confirmed by Wittwer et al by employing finite element simulations to mimic the conditions in RT-DC experiments. 39 Son derived a formula to determine the wall shear rate in a rectangular channel for a power law liquid at a given flow rate Q : 40 where W is the channel width, H is the channel height and H < W , a * and b * are geometrical factors dependent on the aspect ratio of the channel H / W and were determined numerically (for a list of values, see Son 40 ) and n is the flow behavior index. By inserting eqn (12) into eqn (4), one can calculate the effective viscosity inside the channel.…”

“…That this is a valid approach was recently confirmed by Wittwer et al by employing finite element simulations to mimic the conditions in RT-DC experiments. 39 Son derived a formula to determine the wall shear rate in a rectangular channel for a power law liquid at a given flow rate Q: 40…”

Shear rheology of methyl cellulose based solutions for cell mechanical measurements at high shear rates

“…That this is a valid approach was recently confirmed by Wittwer et al by employing finite element simulations to mimic the conditions in RT-DC experiments. 39 Son derived a formula to determine the wall shear rate in a rectangular channel for a power law liquid at a given flow rate Q: 40 where W is the channel width, H is the channel height and H < W , a * and b * are geometrical factors dependent on the aspect ratio of the channel H / W and were determined numerically (for a list of values, see Son 40 ) and n is the flow behavior index. By inserting equation (12) into equation (4), one can calculate the effective viscosity inside the channel.…”

“…That this is a valid approach was recently confirmed by Wittwer et al by employing finite element simulations to mimic the conditions in RT-DC experiments. 39 Son derived a formula to determine the wall shear rate in a rectangular channel for a power law liquid at a given flow rate ܳ: 40…”

High-throughput viscoelastic characterization of cells in hyperbolic microchannels

“…However, the quantification of stresses and cell strain in the microchannels remains challenging due to the complex three-dimensional stress distribution on the cell surface. 18,19 Viscoelastic analysis typically necessitates a onedimensional stress-strain relationship to derive mechanistic properties such as elastic moduli, relaxation times, or storage and loss moduli. 5,10,20,21 Moreover, in cell mechanics measurements, polymeric crowding agents are often employed to enhance the viscosity of the cell carrier solution.…”