A new intracellular serine protease inhibitor expressed in the rat pituitary gland complexes with granzyme B

Hill, Rena M.; Morresey, Kate S; Coates, Leigh C.; Mezey, Éva; Fell, Brennan; Bratt, Tomas; Trapani, Joseph A.; Birch, Nigel P.

doi:10.1016/s0014-5793(98)01475-6

Cited by 8 publications

(6 citation statements)

References 28 publications

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“…Thus, we can infer that the overexpression of G6PD in PMFs contributes to their proliferative advantage over MF‐HSCs. The protein “similar to serine protease inhibitor 6”, also named raPIT5a, the equivalent of rat/mouse serine protease inhibitor 6 and of human PI 9 27, is a granzyme B inhibitor 28. Granzyme B is secreted by natural killer (NK) cells and causes cell death.…”

Section: Discussionmentioning

confidence: 99%

Distinct proteomic features of two fibrogenic liver cell populations: Hepatic stellate cells and portal myofibroblasts

et al. 2010

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In chronic liver diseases, the accumulation of extracellular matrix leading to fibrosis is caused by myofibroblasts, the origins of which are debatable. We performed a comparative proteomic study to identify markers and gain insight into distinct functions of myofibroblasts derived either from hepatic stellate cells (HSCs) or from portal mesenchymal cells. After isolation from normal liver and culture in similar conditions, myofibroblastic HSCs (MF-HSCs) presented enlarged cytoplasms whereas portal myofibroblasts (PMFs) were more proliferative, and formed more stress fibers. The two cell types were subjected to comparative analyses by 2-D MS/MS. Six proteins were overexpressed in PMFs, with myofibroblast-related typical functions. Among them, cofilin-1 showed the greatest difference in expression and a lower pI than expected. Immunoblot demonstrated higher levels of phosphorylation, a modification of the protein implicated in stress fiber formation. Eleven proteins, mostly involved in stress response, were overexpressed in MF-HSCs. Cytoglobin had the highest level of overexpression, as confirmed by reverse transcription quantitative real-time PCR, immunoblot and immunocytochemical analyses. These results identify cytoglobin as the best marker for distinguishing MF-HSCs from PMFs and suggest different functions for the two cell populations in the liver wound healing response, with a prominent role for PMFs in scar formation.

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Section: Discussionmentioning

confidence: 99%

Distinct proteomic features of two fibrogenic liver cell populations: Hepatic stellate cells and portal myofibroblasts

et al. 2010

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“…(1) PAI-2, a macrophage-derived serpin, protects against TNF-induced apoptosis in addition to its role in regulating extracellular urokinase, (2) PI-9, an intracellular serpin present in T lymphocytes, inhibits granzyme B and protects the cell against perforin and granzyme Bmediated killing, and (3) raPIT5a, a serpin that seems to regulate granzyme B-mediated apoptosis in the pituitary gland [35,36,43,44].…”

Section: Discussionmentioning

confidence: 99%

Identification of centerin: a novel human germinal center B cell-restricted serpin

Frazer

Jackson

J³

et al. 2000

Eur. J. Immunol.

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For naive B cells to mature in response to antigen triggering and become either plasma cells or memory B cells, a complex array of events takes place within germinal centers (GC) of secondary lymphoid organs. With the long‐term objective of defining and characterizing molecules that control the generation of GC, we have subtracted RNA messages derived from highly purified B cells at the follicular mantle stage of differentiation from GC B cells. Using this approach, we have identified a novel molecule, centerin, belonging to the family of serine‐protease inhibitors or serpins. Transcription of centerin is highly restricted to GC B cells and their malignant counterparts, Burkitt's lymphoma lines. The putative centerin protein shares the highest sequence identity with thyroxine‐binding globulin and possesses arginine / serine at its P1 / P1′ active site, suggesting that it interacts with a trypsin‐like protease(s). In addition, several other sequence features of centerin also indicate that it serves as a bonafide protease inhibitor. Finally, we demonstrate differentially up‐regulated transcription of this novel gene by resting, naive B cells stimulated in vitro via CD40 signaling, while Staphylococcus aureus Cowan strain‐mediated B cell activation fails to generate this reponse. Because CD40 signaling is required for naive B cells to enter the GC reaction and for GC B cells to survive, it is likely that centerin plays a role in the development and / or sustaining of GC.

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“…The functions of these proteins remain unclear but may involve the stabilization of extracellular matrices (Huang et al, 1993), and more recently, in the case of the larger family, a role in inhibiting apoptosis (Hill et al, 1998; Stahler and Roemer, 1998). ITI heavy chains are expressed abundantly in the liver and contain a putative binding site for hyaluronic acid (HA).…”

Section: Introductionmentioning

confidence: 99%

Itih‐4, a serine protease inhibitor regulated in interleukin‐6–dependent liver formation: role in liver development and regeneration

Bhanumathy

Tang

Monga

et al. 2001

Developmental Dynamics

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Inter-␣-trypsin inhibitor-4 (Itih-4) is a liver-restricted member of the serine protease inhibitor family with diverse functions as an anti-apoptotic and matrix stabilizing molecule that are important throughout development. We investigate the functional role of Itih-4 in liver formation, regeneration (LR) and examine its role in calcium and hyaluronic acid binding. Itih-4 expression is prominent in early liver development at E9 and later at E16, being restricted to hepatoblasts, immature hepatocytes, and differentiated hepatocytes. We note a marked and differential increase in Itih-4 labeling in proliferating hepatocytes, compared with bile duct cells in liver explant cultures treated with interleukin-6 (IL-6). After partial hepatectomy, maximal Itih-4 expression occurs in a bimodal manner at 30 min and at 12 hr, with a predominant centrizonal distribution. There is no detectable binding of glutathione transferase-fusion Itih-4 protein to calcium and hyaluronic acid, indicating a possible requirement for posttranslational modifications for these functions. These results suggest that in LR, Itih-4 expression corresponds to that of immediate early genes and may contribute to the entry of normally quiescent hepatocytes into the early stages of the cell cycle. The markedly high expression of Itih-4 in early liver development and in explants treated with IL-6 suggests a prominent role for Itih-4 at key points in liver formation.

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A new intracellular serine protease inhibitor expressed in the rat pituitary gland complexes with granzyme B

Cited by 8 publications

References 28 publications

Distinct proteomic features of two fibrogenic liver cell populations: Hepatic stellate cells and portal myofibroblasts

Distinct proteomic features of two fibrogenic liver cell populations: Hepatic stellate cells and portal myofibroblasts

Identification of centerin: a novel human germinal center B cell-restricted serpin

Itih‐4, a serine protease inhibitor regulated in interleukin‐6–dependent liver formation: role in liver development and regeneration

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