A New Kaempferol Glycoside with Antioxidant Activity from Chenopodium ambrosioides Growing in Egypt

Ghareeb, Mosad A.; Saad, Amal M.; Abdou, Allia M.; Refahy, Laila A.; Ahmed, Wafaa Sabry

doi:10.13005/ojc/320626

Cited by 26 publications

(17 citation statements)

References 29 publications

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“…On the other hand, however, its value is appreciable and antioxidant activity can be attributed. This finding is in agreement with other studies reported in the literature for antioxidant activity of C. ambroisoides [ 47 , 48 ]. Removal of free radicals is positively associated with increased differentiation and proliferation of osteoblasts [ 49 ], which may explain the effects of C. ambroisoides graft on early bone healing.…”

Section: Discussionsupporting

confidence: 94%

Chenopodium ambrosioides as a bone graft substitute in rabbits radius fracture

Neto

Ribeiro

Morais

et al. 2017

BMC Complement Altern Med

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BackgroundBone defects caused by trauma, infection or tumor resection are common in orthopedic clinic and depending on the extent of the fracture; the vast majority require treatment with bone substitutes. Among the bone grafts employees, the autograft is defined as the gold standard, but with some limitations, principally related to morbidity at the site of its removal, with the need to search for other biomaterials as adjuvant in bone regeneration. Therefore, the objective of the present study was to evaluate the use of Chenopodium ambrosioides as a bone graft substitute for the osseointegration of fractures in rabbits, compared to other bone grafts already employed in the surgical routine as Ricinus communis (castor oil) polyurethane and autogenous bone marrow.MethodsForty-eight rabbits were divided into four groups. After anesthesia, a radius fracture was created and the animals were treated with the following grafts: C. ambrosioides graft, autogenous bone marrow, castor oil and saline (control). After 30, 60 and 90 days, the animals were submitted to radiographical and histological analyses and bone alkaline phosphatase, osteocalcin, biomechanical tension, and collagen were measured. We also realized a phytochemical screening and in vitro antioxidant activity including 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical.ResultsThe data showed that the growth of the bone callus was more expressive and biomechanical assessment showed better tensile strength in C. ambrosioides graft. The experimental results also revealed that there was significantly greater activity of the bone alkaline phosphatase and osteocalcin during early fracture healing, similarly to the group receiving autogenous bone marrow. Histologically, immature bone was observed in C. ambrosioides graft at 30 days, whereas the formation of cartilaginous tissue predominated in the other groups. A higher amount of type I collagen was observed in C. ambrosioides graft throughout treatment. It was detected strong presence of flavonoids and appreciable antioxidant activity.ConclusionsThe results indicate that C. ambrosioides graft and autogenous bone marrow have similar ability to enhance bone regeneration, higher than the castor oil graft, suggesting that the medicinal plant can provide therapeutic benefits for bone tissue.

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Section: Discussionsupporting

confidence: 94%

Chenopodium ambrosioides as a bone graft substitute in rabbits radius fracture

Neto

Ribeiro

Morais

et al. 2017

BMC Complement Altern Med

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“…The experiment was performed in triplicate. The percentage scavenging of the extract was calculated [ 42 , 43 , 44 ].…”

Section: Methodsmentioning

confidence: 99%

In Vitro and In Vivo Antitumor Activity of Indolo[2,3-b] Quinolines, Natural Product Analogs from Neocryptolepine Alkaloid

et al. 2021

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Neocryptolepine (5-methyl-5H-indolo[2,3-b] quinoline) analogs were synthesized and evaluated in vitro and in vivo for their effect versus Ehrlich ascites carcinoma (EAC). The analogs showed stronger cytotoxic activity against EAC cells than the reference drug. The in vivo evaluation of the target compounds against EAC-induced solid tumor in the female albino Swiss mice revealed a remarkable decrease in the tumor volume (TV) and hepatic lipid peroxidation. A noticeable increase of both superoxide dismutase (SOD) and catalase (CAT) levels was reported (p < 0.001), which set-forth proof of their antioxidant effect. In addition, the in vitro antioxidant activity of the neocryptolepine analogs was screened out using the DPPH method and showed promising activities activity. The histopathological investigations affirmed that the tested analogs have a remarkable curative effect on solid tumors with minimal side-effect on the liver. The study also includes illustrated mechanism of the antitumor activity at the cell level by flow cytometry. The cell cycle analysis showed that the neocryptolepine analogs extensively increase the aggregation of tumor cells in three phases of the cell cycle (G0/G1, S and G2/M) with the emergence of a hypo-diploid DNA content peak (sub-G1) in the cell cycle experiments, which is a clear-cut for the apoptotic cell population. Furthermore, the immunological study manifested a significant elevation in splenic lymphocyte count (p < 0.001) with the elevation of the responsiveness of lymphocytes to phytohemagglutinin (PHA). These results indicate that these naturally-based neocryptolepine alkaloids exhibit marked antitumor activity in vivo and represent an important lead in the development of natural-based anticancer drugs.

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“…The ethyl acetate-soluble fraction (55 mg) was purified by PTLC using petroleum ether:ethyl acetate (9:1) as eluent to yield compound 2 (15.8 mg) (Figure 2). 13 C-NMR data (Table 5) [32].…”

Section: Isolation Of Compounds From the Meoh Extract Of M Officinalis Seedsmentioning

confidence: 99%

Phytochemical studies of Melilotus officinalis

Sisay¹,

Mammo²,

Yaya³

2021

Bull. Chem. Soc. Eth.

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GC-MS analysis of the n-hexane extract of Melilotus officinalis seeds revealed twelve compounds with a combined area percentage of 98.33% predominantly, (9Z,12Z)-octadecadienoic acid (20.22%, 366 ppm), 14-methylpentadecanoic acid (19.52%, 353 ppm) and (9E)-octadecenoic acid (15.94%, 289 ppm). Two compounds, namely, cis-coumaric acid-2-O-β-D-glucopyranoside (cis-melilotoside, 1) and 1,2-benzopyrone (coumarin, 2), were isolated from the MeOH extract of the seeds of M. officinalis. The structures of isolated compounds were determined by spectroscopic techniques such as NMR, UV-Vis, and FTIR. The MeOH extract of M. Officinalis was also tested for its antioxidant activity using DPPH assay. The extract showed 29.87% DPPH inhibition at concentration of 100 μg/mL. KEY WORDS: Melilotus officinalis, Fatty acid methyl esters, Esterification, GC-MS, DPPH radical scavenging assay, Antioxidant activity Bull. Chem. Soc. Ethiop. 2021, 35(1), 141-150. DOI: https://dx.doi.org/10.4314/bcse.v35i1.12

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A New Kaempferol Glycoside with Antioxidant Activity from Chenopodium ambrosioides Growing in Egypt

Cited by 26 publications

References 29 publications

Chenopodium ambrosioides as a bone graft substitute in rabbits radius fracture

Chenopodium ambrosioides as a bone graft substitute in rabbits radius fracture

In Vitro and In Vivo Antitumor Activity of Indolo[2,3-b] Quinolines, Natural Product Analogs from Neocryptolepine Alkaloid

Phytochemical studies of Melilotus officinalis

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