A new label-free impedimetric aptasensor for gluten detection

Malvano, Francesca; Albanese, Donatella; Pilloton, Roberto; Matteo, Marisa Di

doi:10.1016/j.foodcont.2017.03.033

Cited by 51 publications

(32 citation statements)

References 22 publications

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“…It is widely used in the biomedical field as carrier systems for drugs, genes, and vaccines, in drug release and targeted drug delivery (Alibolandi et al, 2017;Sharma et al, 2017). Rely on their large numbers of terminal functional groups, PAMAM can greatly increase the quantity of attached various biomolecules such as antibody, enzyme, or aptamer, and consequently improve sensitivity of detection (Akter, Jeong, Lee, Choi, & Rahman, 2017;Malvano, Albanese, Pilloton, & Matteo, 2017;Miodek, Omrani, Khoder, & Youssoufi, 2016). Meanwhile, it was also extensively applied in electrochemical sensor in order to additionally amplify the sensitivity and reach a low detection limit through association with others conductive nanomaterials such as AuNPs, Ag nanoparticles, grapheme, and so on (Araque et al, 2014;Karadag, Geyik, Demirkol, Ertas, & Timur, 2013;Ning, Zhang, & Zheng, 2014).…”

Section: Introductionmentioning

confidence: 99%

A signal-enhanced lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A

Peng

Kang

Pang

et al. 2017

Food and Agricultural Immunology

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A signal-enhanced lateral flow strip biosensor has been constructed for ultrasensitive and on-site visual detection of bisphenol A (BPA). The signal amplification principle is based on the capacity of binding a large number of antibody-assembled gold nanoparticle (AuNP-antibody) conjugates of the poly amidoamine (PAMAM), which bears a multitude of amino groups. This signal-enhanced method could achieve BPA detection at 10 ppb based on naked eye visual observation, which represents an at least 50-fold improvement of the sensitivity of a traditional method without PAMAM. Moreover, good linearity was observed in the range from 2 to 100 ppb after quantitative analysis using the ImageJ software. This signal amplification method could be adopted as a potential generous technique in different fields. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

A signal-enhanced lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A

Peng

Kang

Pang

et al. 2017

Food and Agricultural Immunology

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“…4 origins (protein, carbohydrates) which are characterized by generating an activation of the immune system when certain predisposed people are in contact with them [19][20][21].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Paper based analytical device modified with nanoporous material for the fluorescent sensing of gliadin content in different food samples

Marín-Barroso

Moreira

Messina

et al. 2018

Microchemical Journal

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A novel fluorescent paper based immunosensor for the quantification of gliadin content in different food samples was constructed. The device consists of a paper platform modified with amino functionalized mesoporous material. The nanoporous structure and the aminofunctionality increased the anti-gliadin antibodies immobilization capacity of the sensing surface, conferring high sensitivity to the system. The detection limit reached by the described system allowed us to address the control of gluten free foods, which is extremely important to maintain the food safe consumption by patients with celiac disease, wheat allergy measured by immunoglobulin E, non-celiac gluten intolerance and Dermatitis herpetiformis or Duhring disease. The gliadin determination was performed by applying a noncompetitive immunoassay format, where gliadin present in food samples was recognized by anti-gliadin antibodies immobilized on the mesoporous material and quantified by the addition of anti-gliadin antibody labelled with peroxidase, its substrate: hydrogen peroxide and a mediator: 10-acetyl-3,7-dihydrofenoxacin. This mediator by the action of the enzyme generates resorufin, which was excited by a light emitting diode at 550 nm and emitted a signal at 580nm. The calibration curve obtained for gliadin exhibited a linear range between 0-160 µg Kg-1 and a method detection limit of 0.025 mg Kg-1. The obtained values for relative recovery varied between 98.65% and 102.33% for samples enriched with gliadin. Also, the results suggested that the developed fluorescent paper based immunosensor showed good reproducibility and stability, indicating its applicability for high-sensitive gluten free food control analysis.

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“…Current techniques utilized in detecting this biomarker in food samples is typically incapacitated in detecting the maximum acceptable detection limit of 20 ppm. Aptamers have been developed in recent years with superior sensitivity compared to antibody‐based sensors for the detection of 33‐mer peptide, the most immunodominant fragment of α2‐gliadin, the allergenic protein found in some cereals which trigger celiac diseases (López‐López, Miranda‐Castro, de‐los‐Santos‐Alvarez, Miranda‐Ordieres, & Lobo‐Castañón, ; Malvano, Albanese, Pilloton, & Di Matteo, ). Further, Wang, Wang, Wang, and Chen () recently developed an aptamer‐based resonance light scattering approach for the detection of kanamycin in milk samples.…”

Section: Structure‐informed Detection and Quantitation Of Peptidesmentioning

confidence: 99%

Structure-informed detection and quantification of peptides in food and biological fluids

et al. 2017

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Peptides with biological properties, that is, bioactive peptides, are a class of biomolecules whose health-promoting properties are increasingly being exploited in food and health products.However, research on targeted techniques for the detection and quantification of these peptides is still in its infancy. Such information is needed in order to enhance the biological and chemometric characterization of peptides and their subsequent application in the functional food and pharmaceutical industries. In this review, the role of classic techniques such as electrophoretic, chromatographic, and peptide mass spectrometry in the structure-informed detection and quantitation of bioactive peptides are discussed. Prospects for the use of aptamers in the characterization of bioactive peptides are also discussed. Practical applicationsAlthough bioactive peptides have huge potential applications in the functional foods and health area, there are limited techniques in enhancing throughput detection, quantification, and characterization of these peptides. This review discusses state-of-the-art techniques relevant in complementing bioactive detection and profiling irrespective of the small number of amino acid units. Insights into challenges, possible remedies and prevailing areas requiring thorough research in the extant literature for food chemists and biotechnologists are also presented.

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A new label-free impedimetric aptasensor for gluten detection

Cited by 51 publications

References 22 publications

A signal-enhanced lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A

A signal-enhanced lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A

Paper based analytical device modified with nanoporous material for the fluorescent sensing of gliadin content in different food samples

Structure-informed detection and quantification of peptides in food and biological fluids

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