A signal-enhanced lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A

Peng, Xiayu; Kang, Lichao; Pang, Fangqin; Li, Hongmin; Luo, Rui; Luo, Xiaofeng; Sun, Fenglian

doi:10.1080/09540105.2017.1365822

Cited by 26 publications

(6 citation statements)

References 33 publications

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“…These biosensor developments are very promising, yet mostly lack sensitivity, compared to antibody-based systems. A very comparable signal-enhanced LFIA was proposed by Peng et al [34], with a comparable vLOD of 10 µg/L; yet the cLOD, after picture analysis by ImageJ software, reached only 2 µg/L. An LFIA described by Dzantiev et al [35], employing latex particles, reached a comparable vLOD (5 µg/L).…”

Section: Discussionsupporting

confidence: 56%

Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products

Raysyan

Schneider

2021

Biosensors

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One of the most important chemicals used in the production of polymer plastics and coatings is bisphenol A. However, despite the large number of studies on the toxicity and hormonal activity of BPA, there are still open questions and thus considerable media attention regarding BPA toxicity. Hence, it is necessary to develop a sensitive, simple, cost-efficient, specific, portable, and rapid method for monitoring bisphenol A and for high sample throughput and on-site screening analysis. Lateral flow immunoassays have potential as rapid tests for on-site screening. To meet sensitivity criteria, they must be carefully optimized. A latex microparticle-based LFIA for detection of BPA was developed. The sensitivity of the assay was improved by non-contact printing of spot grids as the control and test lines with careful parameter optimization. Results of the test could be visually evaluated within 10 min with a visual cut-off of 10 µg/L (vLOD). Alternatively, photographs were taken, and image analysis performed to set up a calibration, which allowed for a calculated limit of detection (cLOD) of 0.14 µg/L. The method was validated for thermal paper samples against ELISA and LC–MS/MS as reference methods, showing good agreement with both methods.

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Section: Discussionsupporting

confidence: 56%

Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products

Raysyan

Schneider

2021

Biosensors

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“…Shen et al designed gold nanoparticle aggregates based on PAMAM to enhance the sensitivity of antibody-based strips for macromolecules; a result almost 20-fold lower than the traditional method using individual gold nanoparticles was achieved [ 18 ]. Similarly, we previously developed a signal-enhanced strip biosensor for small molecule detection based on PAMAM binding with antibodies [ 19 ]. This signal-enhanced method exhibited at least a 50-fold improvement of the sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Design of a Signal-Amplified Aptamer-Based Lateral Flow Test Strip for the Rapid Detection of Ochratoxin A in Red Wine

Liu

Liú

Cui

et al. 2022

Foods

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In order to improve the weak optical performance of gold nanoparticles and realize the signal amplification of lateral flow chromatography test strips, individual gold nanoparticles (AuNPs) were aggregated into gold nanoparticle aggregates through functional groups around polyamidoamine (PAMAM) dendrimers. A signal-amplified aptamer-based lateral flow chromatography test strip was constructed for the rapid determination of ochratoxin A (OTA). Under optimal conditions, the visual detection limit of this test strip was 0.4 ng mL−1 and the semi-quantitative limit of detection (LOD) was 0.04 ng mL−1. Compared with other traditional aptamer lateral flow chromatography test strips, its sensitivity was improved about five times. The whole test could be completed within 15 min. The aptamer-based strip was applied to the detection of OTA in red wine; the average recoveries ranged from 93% to 105.8% with the relative standard deviation (RSD) varying from 3% to 8%, indicating that the test strip may be a potentially effective tool for the on-site detection of OTA.

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“…The only limiting factor for LFIA expansion is its moderate sensitivity. The known approaches for improving LFIA sensitivity are extremely diverse (Chen et al, 2015;Gao et al, 2016;Peng et al, 2017;Shan, Lai, Xiong, Wei, & Xu, 2015;Song, Zou, Zhu, Liu, & Kuang, 2017;Xing et al, 2015) and may be based on the use of alternate labels, catalytic enhancement or aggregation processes. There is no one accepted method of amplification that can be unambiguously recommended as the optimal method for all LFIAs. This article presents the development of a highly-sensitive, enhanced technique for LFIA of potato leafroll virus (PLRV).…”

Section: Introductionmentioning

confidence: 99%

Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus

Panferov

Safenkova

Byzova

et al. 2017

Food and Agricultural Immunology

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Rapid non-laboratory screening of plants for pathogenic viruses crucially influences crop yields in modern agricultural technologies. The aim of this study was to develop a highly-sensitive lateral flow immunoassay (LFIA) for rapid detection of potato leafroll virus (PLRV), an infectious agent of one of the most widespread potato diseases. The proposed LFIA combines the formation of sandwich immune complexes with gold nanoparticles (GNP) as labels and silver enhancement. The enhancement stage was realized using mixture of silver lactate and hydroquinone and subsequent addition of chloride-containing buffer to stop silver reduction. LFIA with silver enhancement was 15 times more sensitive (detection limit 0.2 ng/mL; 15 min) compared with conventional LFIA (detection limit 3 ng/mL; 10 min). The enhanced LFIA detected PLRV in leaves' extracts of infected potato in dilutions higher than enzyme-linked immunosorbent assay.

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A signal-enhanced lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A

Cited by 26 publications

References 33 publications

Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products

Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products

Design of a Signal-Amplified Aptamer-Based Lateral Flow Test Strip for the Rapid Detection of Ochratoxin A in Red Wine

Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus

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