A New LC/MS/MS Method for the Analysis of Phyllanthin in Rat Plasma and its Application on Comparative Bioavailability of Phyllanthin in Different Formulations after Oral Administration in Rats

Abstract

• A rapid and selective ultra performance liquid chromatography (UPLC) with detector MS/ MS to quantify phyllanthin in rat plasma in 1.5 minutes is described. • Phyllanthin is extracted from a small volume of rat plasma (100 µl) by means of liquid-liquid extraction method. • The validated method was successfully applied to pharmacokinetic studies of oral administration of different phyllanthin formulations to rats. • Phyllanthin from complex with phospholipid showed significantly higher AUC, C max and a longer half-life than that from raw Phyllanthus amarus extract indicating higher bioavailability of the phyllanthin-phospholipid complex.