A new mapping method for quantitative trait loci of silkworm

Xu, Haiming; Wei, Colin; Tang, Yunting; Zhu, Zhiliang; Sima, Yang-Fu; Lou, Xiang‐Yang

doi:10.1186/1471-2156-12-19

Cited by 14 publications

(15 citation statements)

References 55 publications

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“…They also elucidated genetic diversity among the temperate silkworm races for developing commercial silkworm hybrids to harness the hybrid vigour. Xu et al (2011) used a mixed linear model including QTL main effects, epistatic effects and QTL £ sex interaction effects for mapping QTLs in an F 2 population of silkworms and found that a sample size of 100 individuals was sufficient to get markers stably associated with QTLs. Since bivoltine silkworm hybrids cannot survive the harsh climatic conditions in the tropics, especially during the summer and rainy months, hardy tropical silkworm hybrids need to be developed.…”

Section: Discussionmentioning

confidence: 99%

Diversity and marker association in tropical silkworm breeds of Bombyx mori (Lepidoptera: Bombycidae)

Srivastava

Vijayan

Kar

et al. 2011

Int. J. Trop. Insect Sci.

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Inter simple sequence repeats (ISSR) and random amplified DNA polymorphism (RAPD) markers were used to estimate the genetic diversity among 14 tropical silkworm races (Bombyx mori L.) for identifying potential parents to be used for hybrid preparation for commercial exploitation. High polymorphism (70.91 and 74.70%) was revealed by ISSR and RAPD markers. The dendrograms generated, using unweighted pair group method using arithmetic average from these markers, grouped the 14 silkworm races into two major groups, which corroborates the differences in cocoon characteristics. Discriminant function analysis of ISSR and RAPD markers identified three functions for cocoon weight and two functions for shell weights, respectively.Step-wise multiple regression analysis identified six ISSR markers () significantly associated with cocoon and shell weights. The genetically divergent parents, identified through this study, can be used for the preparation of hybrids for commercial utilization. Similarly, the DNA markers identified can be utilized for markerassisted selection along with those identified through quantitative trait locus mapping by others. Thus the information generated in this study is useful in silkworm breeding programmes in the tropical sericulture industry.

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Section: Discussionmentioning

confidence: 99%

Diversity and marker association in tropical silkworm breeds of Bombyx mori (Lepidoptera: Bombycidae)

Srivastava

Vijayan

Kar

et al. 2011

Int. J. Trop. Insect Sci.

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“…Linkage maps with microsatellites have allowed us to advance in the knowledge of characteristics of economic interest in B. mori, as well as in the Lepidoptera genetic architecture. However, Xu et al 68 and Li et al 69 indicate that the most used models have not been adjusted according to the particular genetic characteristics of the silkworm, because they ignore the effects of gender and the presence of aquiasmatic meiosis, a process that implies the absence of chromosomal cross-linking in the germinal line of the females. As a result, these authors have proposed models that allow correcting the potential biases and lack of precision of the most used methods for mapping QTLs in B. mori.…”

Section: Identification Of Genes and Qtlsmentioning

confidence: 99%

“…As a result, these authors have proposed models that allow correcting the potential biases and lack of precision of the most used methods for mapping QTLs in B. mori. Xu et al 68 propose a statistical model to analyze QTLs in F2 populations, while Li et al 69 focus their method on the use of backcrossing and the rational selection of mapping populations to first identify the chromosomes with QTLs and subsequently their position.…”

Section: Identification Of Genes and Qtlsmentioning

confidence: 99%

Role of microsatellites in genetic analysis of Bombyx mori silkworm: a review

et al. 2019

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In the genome of Bombyx mori Linnaeus (1758), the microsatellites, or simple sequence repeats (SSR), feature among their particular characteristics a high adenine and thymine (A/T) content, low number of repeats, low frequency, and a grouping in "families" with similar flanking regions. Such characteristics may be the result of a complex interaction between factors that limit the size and dispersion of SSR loci—such as their high association with transposons—and mean that microsatellites within this taxon suitable as molecular markers are relatively rare. The determination of genetic profiles in populations and cell lines has not been affected owing to the high level of polymorphism, nor has the analysis of diversity, structure and genetic relationships. However, the scarcity of suitable microsatellites has restricted their application in genetic mapping, limiting them to preliminary identification of gene location of genes or quantitative trait loci (QTLs) related to thermotolerance, resistance to viruses, pigmentation patterns, body development and the weight of the cocoon, the cortex, the pupa and the filament. The review confirms that, as markers, microsatellites are versatile and perform well. They could thus be useful both to advance research in emerging countries with few resources seeking to promote sericulture in their territories, and to advance in the genetic and molecular knowledge of characteristics of productive and biological interest, given the latest technological developments in terms of the sequencing, identification, isolation and genotyping of SSR loci.

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“…Xu et al . () proposed a new statistical model to analyze QTL in silkworm F 2 populations in which the achiasmatic and gender effects were considered. However, several issues with this proposed model still need to be resolved.…”

Section: Introductionmentioning

confidence: 99%

A composite method for mapping quantitative trait loci without interference of female achiasmatic and gender effects in silkworm,Bombyx mori

Zuo

Tong

et al. 2015

Anim Genet

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The silkworm, Bombyx mori, is an economically important insect that was domesticated more than 5000 years ago. Its major economic traits focused on by breeders are quantitative traits, and an accurate and efficient QTL mapping method is necessary to explore their genetic architecture. However, current widely used QTL mapping models are not well suited for silkworm because they ignore female achiasmate and gender effects. In this study, we propose a composite method combining rational population selection and special mapping methods to map QTL in silkworm. By determining QTL for cocoon shell weight (CSW), we demonstrated the effectiveness of this method. In the CSW mapping process, only 56 markers were used and five loci or chromosomes were detected, more than in previous studies. Additionally, loci on chromosomes 1 and 11 dominated and accounted for 35.10% and 15.03% of the phenotypic variance respectively. Unlike previous studies, epistasis was detected between loci on chromosomes 11 and 22. These mapping results demonstrate the power and convenience of this method for QTL mapping in silkworm, and this method may inspire the development of similar approaches for other species with special genetic characteristics.

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A new mapping method for quantitative trait loci of silkworm

Cited by 14 publications

References 55 publications

Diversity and marker association in tropical silkworm breeds of Bombyx mori (Lepidoptera: Bombycidae)

Diversity and marker association in tropical silkworm breeds of Bombyx mori (Lepidoptera: Bombycidae)

Role of microsatellites in genetic analysis of Bombyx mori silkworm: a review

A composite method for mapping quantitative trait loci without interference of female achiasmatic and gender effects in silkworm,Bombyx mori

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