Diversity and marker association in tropical silkworm breeds of Bombyx mori (Lepidoptera: Bombycidae)

Abstract

Inter simple sequence repeats (ISSR) and random amplified DNA polymorphism (RAPD) markers were used to estimate the genetic diversity among 14 tropical silkworm races (Bombyx mori L.) for identifying potential parents to be used for hybrid preparation for commercial exploitation. High polymorphism (70.91 and 74.70%) was revealed by ISSR and RAPD markers. The dendrograms generated, using unweighted pair group method using arithmetic average from these markers, grouped the 14 silkworm races into two major groups, which corroborates the differences in cocoon characteristics. Discriminant function analysis of ISSR and RAPD markers identified three functions for cocoon weight and two functions for shell weights, respectively.Step-wise multiple regression analysis identified six ISSR markers () significantly associated with cocoon and shell weights. The genetically divergent parents, identified through this study, can be used for the preparation of hybrids for commercial utilization. Similarly, the DNA markers identified can be utilized for markerassisted selection along with those identified through quantitative trait locus mapping by others. Thus the information generated in this study is useful in silkworm breeding programmes in the tropical sericulture industry.

Effect of Climatic Conditions and Land Cover on Genetic Structure and Diversity of Eunica tatila (Lepidoptera) in the Yucatan Peninsula, Mexico

“…In addition, microsatellites are inherently difficult to use in butterflies, partially due to the high frequency of null alleles [49]. Therefore, ISSR genetic markers have been widely applied in studies on butterfly population genetic [15][16][17]43,48,[50][51][52][53][54][55][56][57].…”

Discovery of a New Species of Telenomus (Hymenoptera: Scelionidae) Parasitic on Eggs of Bombyx mandarina and Bombyx mori (Lepidoptera: Bombycidae) in Japan and Taiwan

“…The genus Bombyx Linnaeus (Lepidoptera: Bombycidae) contains six species, B. huttoni Westwood, B. horsfieldi (Moore), B. incomposita van Eecke, B. lemeepauli Lemée, B. mandarina (Moore), and B. mori (Linnaeus) ( Wang et al 2015 ). These six species are all distributed in Asian countries, except for B. mori, a domesticated species which is cultured around the world for sericulture, insect physiology, and genetics ( Yokoyama 1963 , Horie and Watanabe 1980 , Mita et al 2004 , Goldsmith et al 2005 , Srivastava 2011 , Nation 2015 , Wang et al 2015 ).…”