A new member of the polyomavirus family: the hamster papovavirus. Complete nucleotide sequence and transformation properties.

Delmas, Véronique; Bastien, C.; Scherneck, Siegfried; Feunteun, Jean

doi:10.1002/j.1460-2075.1985.tb03773.x

Cited by 59 publications

(41 citation statements)

References 25 publications

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“…Mammalian hosts also exist that display various mutant glycosylation phenotypes (32). These might be employed in conjunction with lectin and antibody selection schemes (32) and with alternative vector systems (33,34) to isolate specific glycosyltransferase cDNAs. Moreover, sib selection methods based upon sensitive glycosyltransferase assays also provide opportunities for general application of this approach.…”

Section: Discussionmentioning

confidence: 99%

Isolation of a cDNA encoding a murine UDPgalactose:beta-D-galactosyl- 1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase: expression cloning by gene transfer.

Larsen

Rajan

Ruff

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

210

115

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We have developed a genetic approach to isolate cloned cDNA sequences that determine expression of cell surface oligosaccharide structures and their cognate glycosyltransferases. A cDNA library was constructed in a mammalian expression vector by using mRNA from a murine cell line known to express a UDPgalactose:,B-D-galactosyl-1,4-N-acetyl-D-glucosaminide a-1,3-galactosyltransferase [(al-3)GT; EC 2.4.1.151]. This library was transfected into COS-1 cells, which lack expression of (al-3)GT. Transfected cells containing functional (al-3)GT cDNAs were detected and isolated with a lectin that recognizes the surface-expressed glycoconjugate product of the (al-3)GT enzyme. One cloned (al-3)GT cDNA was rescued from lectin-positive transfected cells. This cDNA contains a single long open reading frame that predicts a 394-amino-acid protein. No significant primary structure similarities were identified between this protein and other known sequences. However, the protein predicts a type II transmembrane topology similar to two other mammalian glycosyltransferases. This topology places the large COOH-terminal domain within the Golgi lumen; this domain was shown to be catalytically active when expressed in COS-1 cells as a portion of a secreted protein A fusion peptide. Biochemical analysis confirmed that this enzyme catalyzes a transglycosylation reaction between UDP-Gal and Gal(fi1-4)GlcNAc to form Gal(al-3)Gal(Ji1-4)GlcNAc. This cloning approach may be generally applicable to the isolation ofcDNAs encoding other mammalian glycosyltransferases.

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Section: Discussionmentioning

confidence: 99%

Isolation of a cDNA encoding a murine UDPgalactose:beta-D-galactosyl- 1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase: expression cloning by gene transfer.

Larsen

Rajan

Ruff

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

210

115

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“…This arrangement of gene products is, depending on the point of view, either a quirk or a stroke of evolutionary genius. PyV and hamster polyomavirus (65,80,122) are the only two family members that have MT. Other polyomaviruses (for example, simian virus 40 [SV40] and the human viruses BK and JC) lack it.…”

Section: Introductionmentioning

confidence: 99%

Lessons in Signaling and Tumorigenesis from Polyomavirus Middle T Antigen

Fluck

Schaffhausen

2009

Microbiol Mol Biol Rev

145

156

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SUMMARY The small DNA tumor viruses have provided a very long-lived source of insights into many aspects of the life cycle of eukaryotic cells. In recent years, the emphasis has been on cancer-related signaling. Here we review murine polyomavirus middle T antigen, its mechanisms, and its downstream pathways of transformation. We concentrate on the MMTV-PyMT transgenic mouse, one of the most studied models of breast cancer, which permits the examination of in situ tumor progression from hyperplasia to metastasis.

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“…In this report we present the nucleotide sequence of the complete BPyV genome, which is compared to the sequences of the genomes of SV40 (Fiers et al, 1978) and mouse polyomavirus (PyV; Griffin et al, 1981). In some relevant instances the known sequences of human polyomaviruses BK-Dunlop strain (BKV-dun;Seif et al, 1979) and JC virus (JCV; Frisque et al, 1984), and other animal polyomaviruses, monkey B-lymphotropic polyomavirus (LPV;Pawlita et al, 1985), hamster polyomavirus (HaPV; Delmas et al, 1985) and the budgerigar fledgling disease virus (BFDV; Rott et al, 1988) are also included in the comparison.…”

Section: Introductionmentioning

confidence: 99%

The Complete Nucleotide Sequence of Bovine Polyomavirus

Schuurman

Sol

Noordaa

1990

Journal of General Virology

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The complete sequence of the genome of bovine polyomavirus (BPyV), formerly known as the CK isolate of the stump-tailed macaque virus, is presented. The genomic organization of BPyV is similar to that of the non-rodent polyomaviruses. With a genome size of 4697 bp, BPyV has the smallest polyomavirus genome known so far. When compared to simian virus 40 (SV40), the shortness of the BPyV genome is due mainly to differences in the coding capacity of the BPyV early region. The first exon of the proposed large T antigen encodes only 35 amino acids; also, a coding region corresponding to the C-terminal 64 amino acids of the SV40 large T antigen is absent in BPyV. It is proposed that the nucleotide sequence encompassing the small t antigen coding sequence contains an intron sequence of 71 nucleotides. Together the two exon sequences encode a 124 amino acid protein. We conclude that this may be the first example of a polyomavirus that has a small t antigen which is translated from two exon sequences. The enhancer region of BPyV does not show homology to the SV40 enhancer sequences. An agnogene is present with a coding capacity of 118 amino acid residues. The highest degree of homology to SV40 and PyV is present in the VP1 molecule.

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A new member of the polyomavirus family: the hamster papovavirus. Complete nucleotide sequence and transformation properties.

Cited by 59 publications

References 25 publications

Isolation of a cDNA encoding a murine UDPgalactose:beta-D-galactosyl- 1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase: expression cloning by gene transfer.

Isolation of a cDNA encoding a murine UDPgalactose:beta-D-galactosyl- 1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase: expression cloning by gene transfer.

Lessons in Signaling and Tumorigenesis from Polyomavirus Middle T Antigen

The Complete Nucleotide Sequence of Bovine Polyomavirus

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