A new member of the fusaricidin family – structure elucidation and synthesis of fusaricidin E

Reimann, Marcel; Sandjo, Louis P.; Antelo, Luís T.; Thines, Eckhard; Siepe, Isabella; Opatz, Till

doi:10.3762/bjoc.13.140

Cited by 11 publications

(4 citation statements)

References 22 publications

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“…Additionally, two hitherto unknown fusaricidins were obtained from fermentation broths of Paenibacillus sp. strain Lu16774 [50]. Li et al demonstrated that P. polymyxa SQR-21 could also produce four kinds of fusaricidins, A (883 Da), B (897 Da), C (947 Da), and D (961 Da) [46].…”

Section: Discussionmentioning

confidence: 99%

Fusaricidin Produced by Paenibacillus polymyxa WLY78 Induces Systemic Resistance against Fusarium Wilt of Cucumber

Chen

2019

IJMS

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Cucumber is an important vegetable crop in China. Fusarium wilt is a soil-borne disease that can significantly reduce cucumber yields. Paenibacillus polymyxa WLY78 can strongly inhibit Fusarium oxysporum f. sp. Cucumerium, which causes Fusarium wilt disease. In this study, we screened the genome of WLY78 and found eight potential antibiotic biosynthesis gene clusters. Mutation analysis showed that among the eight clusters, the fusaricidin synthesis (fus) gene cluster is involved in inhibiting the Fusarium genus, Verticillium albo-atrum, Monilia persoon, Alternaria mali, Botrytis cinereal, and Aspergillus niger. Further mutation analysis revealed that with the exception of fusTE, the seven genes fusG, fusF, fusE, fusD, fusC, fusB, and fusA within the fus cluster were all involved in inhibiting fungi. This is the first time that demonstrated that fusTE was not essential. We first report the inhibitory mode of fusaricidin to inhibit spore germination and disrupt hyphal membranes. A biocontrol assay demonstrated that fusaricidin played a major role in controlling Fusarium wilt disease. Additionally, qRT-PCR demonstrated that fusaricidin could induce systemic resistance via salicylic acid (SA) signal against Fusarium wilt of cucumber. WLY78 is the first reported strain to both produce fusaricidin and fix nitrogen. Therefore, our results demonstrate that WLY78 will have great potential as a biocontrol agent in agriculture.

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Section: Discussionmentioning

confidence: 99%

Fusaricidin Produced by Paenibacillus polymyxa WLY78 Induces Systemic Resistance against Fusarium Wilt of Cucumber

Chen

2019

IJMS

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“…They are non-cationic cyclic lipodepsipeptides composed of guanidinylated beta-hydroxy fatty acids bound to a cyclic hexapeptide [28][29][30][31]. This antibiotic exhibits activity against Gram-positive bacteria such as Staphylococcus aureus and Micrococcus luteus IFO 3333, through interaction with the phospholipids of cell membranes, but have weak activity against gram-negative bacteria [28,30,32]. The V. vulnificus species had an abundance of 24% in the 2018 sample obtained from the Sisal wetland.…”

Section: Identification Of Potential Nrps-producing Bacteria In Coastal Wetlands Of Yucatanmentioning

confidence: 99%

Mining the Yucatan Coastal Microbiome for the Identification of Non-Ribosomal Peptides Synthetase (NRPS) Genes

Martínez-Núñez

Rodríguez-Escamilla

2020

Toxins

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Prokaryotes represent a source of both biotechnological and pharmaceutical molecules of importance, such as nonribosomal peptides (NRPs). NRPs are secondary metabolites which their synthesis is independent of ribosomes. Traditionally, obtaining NRPs had focused on organisms from terrestrial environments, but in recent years marine and coastal environments have emerged as an important source for the search and obtaining of nonribosomal compounds. In this study, we carried out a metataxonomic analysis of sediment of the coast of Yucatan in order to evaluate the potential of the microbial communities to contain bacteria involved in the synthesis of NRPs in two sites: one contaminated and the other conserved. As well as a metatranscriptomic analysis to discover nonribosomal peptide synthetases (NRPSs) genes. We found that the phyla with the highest representation of NRPs producing organisms were the Proteobacteria and Firmicutes present in the sediments of the conserved site. Similarly, the metatranscriptomic analysis showed that 52% of the sequences identified as catalytic domains of NRPSs were found in the conserved site sample, mostly (82%) belonging to Proteobacteria and Firmicutes; while the representation of Actinobacteria traditionally described as the major producers of secondary metabolites was low. It is important to highlight the prediction of metabolic pathways for siderophores production, as well as the identification of NRPS’s condensation domain in organisms of the Archaea domain. Because this opens the possibility to the search for new nonribosomal structures in these organisms. This is the first mining study using high throughput sequencing technologies conducted in the sediments of the Yucatan coast to search for bacteria producing NRPs, and genes that encode NRPSs enzymes.

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“…Fusaricidin is an important antibiotic family produced by Paenibacillus polymyxa with great potential use in medical and agricultural applications ( Kajimura and Kaneda, 1996 ; Lee et al, 2012 ; Haron et al, 2019 ; Jeong et al, 2019 ). So far, 14 fusaricidin members have been reported, consisting of an invariable 15-guanidino-3-hydroxypentadecanoic acid and a variable cyclic hexapeptide ( Deng et al, 2011 ; Vater et al, 2015 ; Reimann et al, 2017 ). The variable cyclic hexadepsipeptide moiety of fusaricidin usually contains six amino acid residues: L-Thr 1 , X 2 , X 3 , D-allo-Thr 4 , X 5 , and D-Ala 6 ( Han et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

“…Among the family, fusaricidin LI-F07a has better antimicrobial activity than other fusaricidin analogs. Based on the current knowledge, L-Thr 1 , D-Val 2 , L-Phe 3 , D-allo-Thr 4 , D-Asn 5 , and D-Ala 6 constitute the cyclic hexadepsipeptide moiety within LI-F07a ( Reimann et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Structure modification of an antibiotic: by engineering the fusaricidin bio-synthetase A in Paenibacillus polymyxa

Li,

Chen

2023

Front. Microbiol.

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Fusaricidin, a lipopeptide antibiotic, is specifically produced by Paenibacillus polymyxa strains, which could strongly inhibit Fusarium species fungi. Fusaricidin bio-synthetase A (FusA) is composed of six modules and is essential for synthesizing the peptide moiety of fusaricidin. In this study, we confirmed the FusA of Paenibacillus polymyxa strain WLY78 involved in producing Fusaricidin LI-F07a. We constructed six engineered strains by deletion of each module within FusA from the genome of strain WLY78. One of the engineered strains is able to produce a novel compound that exhibits better antifungal activity than that of fusaricidin LI-F07a. This new compound, known as fusaricidin [ΔAla6] LI-F07a, has a molecular weight of 858. Our findings reveal that it exhibits a remarkable 1-fold increase in antifungal activity compared to previous fusaricidin, and the fermentation yield reaches ~55 mg/L. This research holds promising implications for plant protection against infections caused by Fusarium and Botrytis pathogen infection.

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A new member of the fusaricidin family – structure elucidation and synthesis of fusaricidin E

Cited by 11 publications

References 22 publications

Fusaricidin Produced by Paenibacillus polymyxa WLY78 Induces Systemic Resistance against Fusarium Wilt of Cucumber

Fusaricidin Produced by Paenibacillus polymyxa WLY78 Induces Systemic Resistance against Fusarium Wilt of Cucumber

Mining the Yucatan Coastal Microbiome for the Identification of Non-Ribosomal Peptides Synthetase (NRPS) Genes

Structure modification of an antibiotic: by engineering the fusaricidin bio-synthetase A in Paenibacillus polymyxa

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