A new method for diagnosing biochemical abnormalities of anterior cruciate ligament (ACL) in human knees: A Raman spectroscopic study

Matsunaga, Ryo; Takahashi, Yasuhito; Takahashi, Riku; Nagao, Toshitaka; Shishido, Toetsu; Tateiwa, Toshiyuki; Pezzotti, Giuseppe; Yamamoto, Kenjiro

doi:10.1016/j.actbio.2019.09.016

Cited by 4 publications

(6 citation statements)

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“…76 Moreover, RS revealed useful in supporting the diagnostic of biochemical abnormalities of the ACL. 77 Schematic representation of in vitro musculoskeletal assessment conducted by PRS technique (as described in reference 69 is reported in Figure 2. Figure 2A shows a scheme of the PRS investigation performed on both old and adult rat tail tendon (RTT) fibres in order to assess the effect of ageing on the tissue.…”

Section: Tendon and Ligamentsmentioning

confidence: 99%

“…123,124 RS can be used intra-operatively to improve surgical treatments for joint diseases or injuries. 80,125 Matsunaga et al 77 reported the application of RS for the non-destructive diagnosis of molecular tissue degeneration, applied to ex vivo human ACL. Encouraging outcomes of this work could promote Raman microscopy as a reliable alternative to the invasive histology or to MRI, which is unable to identify the biochemical components related to degeneration.…”

Section: Status and Challenges For Clinical Translationmentioning

confidence: 99%

“… 75 Moreover, RS was revealed to be useful in supporting the diagnostics of biochemical abnormalities of the ACL. 76 …”

Section: Raman Studies Of Skeletal Tissuesmentioning

confidence: 99%

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Raman Spectroscopy in Skeletal Tissue Disorders and Tissue Engineering: Present and Prospective

Fosca

Basoli

Bella

et al. 2022

Tissue Engineering Part B: Reviews

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Musculoskeletal disorders are the most common reason of chronic pain and disability, representing an enormous socioeconomic burden worldwide. In this review, new biomedical application fields for Raman spectroscopy (RS) technique related to skeletal tissues are discussed, showing that it can provide a comprehensive profile of tissue composition in situ , in a rapid, label-free, and nondestructive manner. RS can be used as a tool to study tissue alterations associated to aging, pathologies, and disease treatments. The main advantage with respect to currently applied methods in clinics is its ability to provide specific information on molecular composition, which goes beyond other diagnostic tools. Being compatible with water, RS can be performed without pretreatment on unfixed, hydrated tissue samples, without any labeling and chemical fixation used in histochemical methods. This review first provides the description of the basic principles of RS as a biotechnology tool and is introduced into the field of currently available RS-based techniques, developed to enhance Raman signals. The main spectral processing, statistical tools, fingerprint identification, and available databases are mentioned. The recent literature has been analyzed for such applications of RS as tendon and ligaments, cartilage, bone, and tissue engineered constructs for regenerative medicine. Several cases of proof-of-concept preclinical studies have been described. Finally, advantages, limitations, future perspectives, and challenges for the translation of RS into clinical practice have been also discussed. Impact statement Raman spectroscopy (RS) is a powerful noninvasive tool giving access to molecular vibrations and characteristics of samples in a wavelength window of 600 to 3200 cm −1 , thus giving access to a molecular fingerprint of biological samples in a nondestructive way. RS could not only be used in clinical diagnostics, but also be used for quality control of tissues and tissue-engineered constructs, reducing number of samples, time, and the variety of analysis required in the quality control chain before implantation.

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Section: Tendon and Ligamentsmentioning

confidence: 99%

Section: Status and Challenges For Clinical Translationmentioning

confidence: 99%

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Raman Spectroscopy in Skeletal Tissue Disorders and Tissue Engineering: Present and Prospective

Fosca

Basoli

Bella

et al. 2022

Tissue Engineering Part B: Reviews

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“…Raman spectroscopy is a label-free method that, compatible with life, enables direct assessments of chemical bonds through their specific vibrational signatures. , In recent years, the Raman method has widely extended toward biomedical applications including quantitative histochemistry, cell/tissue physiology, , and disease diagnostics. − The Raman bands, which represent vibrational energy levels of specific molecular bonds, provide a wealth of information about relative fractions, chemical structures, and structural modifications of biomolecules in relation to their chemical microenvironment. , In a previous paper, we located spectral fingerprints of individual membrane lipids and deoxynucleoside triphosphates in living neuronal cells, and we mapped in situ cell networking and separation at the micrometric level. In this work, we explored the label-free/molecular-selective Raman patterning approach to distinguish between MSCs and NMSCs and to visualize the relevant chemical species involved with the functionality of living Schwann cells in coculture with neuronal cells.…”

Section: Introductionmentioning

confidence: 99%

Raman Probes forIn SituMolecular Analyses of Peripheral Nerve Myelination

Pezzotti

Adachi

Miyamoto

et al. 2020

ACS Chem. Neurosci.

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The myelinating activity of living Schwann cells in coculture with neuronal cells was examined in situ in a Raman microprobe spectroscope. The Raman label-free approach revealed vibrational fingerprints directly related to the activity of Schwann cells' metabolites and identified molecular species peculiar to myelinating cells. The identified chemical species included antioxidants, such as hypotaurine and glutathione, and compartmentalized water, in addition to sphingolipids, phospholipids, and nucleoside triphosphates also present in neuronal and nonmyelinating Schwann cells. Raman maps at specific frequencies could be collected, which clearly visualized the myelinating action of Schwann cells and located the demyelinated ones. An important finding was the spectroscopic visualization of confined water in the myelin structure, which exhibited a quite pronounced Raman signal at ∼3470 cm −1 . This peculiar signal, whose spatial location precisely corresponded to a low-frequency fingerprint of hypotaurine, was absent in unmyelinating cells and in bulk water. Raman enhancement was attributed to frustration in the hydrogen-bond network as induced by interactions with lipids in the myelin sheaths. According to a generally accepted morphological model of myelin, an explanation was offered of the peculiar Raman scattering of water confined in intraperiod lines, according to an ordered hydrogen bonding structure. The possibility of concurrently mapping antioxidant molecules and compartmentalized water structure with high spectral accuracy and microscopic spatial resolution enables probing myelinating activity and might play a key-role in future studies of neuronal pathologies. Compatible with life, Raman microprobe spectroscopy with the newly discovered probes could be suitable for developing advanced strategies in the reconstruction of injured nerves and nerve terminals at neuromuscular junctions.

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“…The ACL is located diagonally in the knee as is shown in the below figure, keeping the tibia from sliding out before the femur and also giving rotational steadiness to the knee. The ACL is one of the four primary ligaments inside the knee that associate the femur to the tibia [5][6][7] . ACL tends to be at risk as it prevents posterior displacement of the distal femur on the tibia.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Quadriceps Muscle Girth Using Ultrasound Imaging In Supervised vs Unsupervised Post Operative ACL Reconstruction

Saxena¹,

Sarkar²,

Naik³

2020

MLU

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Injury to the anterior cruciate ligament (ACL) is one of the most common knee ligament injuries. The ACL injury is a sprain or tear of the anterior ligament (ACL). Effective exercises will reduce the risk of ACL injury. An ACL injury in athletes is a higher incidence than other general public, such as soccer, basketball, hockey, etc. The aim of this studywas to compare the effectiveness, recovery rate and variance in both knee-affected and non-affected muscle girth readings in supervised v/s unsupervised patient groups after the patient underwent ACL reconstruction surgery. The participants were divided into two groups(A-Supervised and B-Unsupervised) through a random sampling. The supervised exercise in group A performed according to the WILK protocol at RML hospital and un-superviseed group B performed the same WILK protocol at home. The ultrasound scans were performed after 2-4 weeks and 12-14 weeks post operation to measure the muscle girth in both the groups. The study findings indicated the significant difference in recovery rate as well as the difference in between supervisedv/s unsupervised groups i.e. the difference of affected and unaffected knees based on ultrasound imaging was higher in un-supervised group compared to supervised group (a difference of 2.3 mm in post-operation muscle girth after 12-14 weeks). The ultrasound reading of the muscle girth after 2-4 weeks post-operation showed a decline in the recovery of the muscle girth in both the groups (9% and 8% for supervised and un-supervised groups respectively). This reduction in the muscle girth value as compared to the pre-operative values was due to arthroscopic effect, which showed there was a transient deactivation of the muscle take place due to surgery. The ultrasound reading of the muscle girth after 12-14 weeks post-operation showed a different progressive recovery of the muscle girth in both the groups as 31% and 10% for supervised and un-supervised groups respectively .We concluded that supervised exercises was better than unsupervised exercises for the early recovery after ACL recontraction.

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A new method for diagnosing biochemical abnormalities of anterior cruciate ligament (ACL) in human knees: A Raman spectroscopic study

Cited by 4 publications

References 50 publications

Raman Spectroscopy in Skeletal Tissue Disorders and Tissue Engineering: Present and Prospective

Raman Spectroscopy in Skeletal Tissue Disorders and Tissue Engineering: Present and Prospective

Raman Probes forIn SituMolecular Analyses of Peripheral Nerve Myelination

Comparison of Quadriceps Muscle Girth Using Ultrasound Imaging In Supervised vs Unsupervised Post Operative ACL Reconstruction

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