A new method for extracting DNA or RNA for polymerase chain reaction

Yamada, Osamu; Toshiya, Matsumoto; Nakashima, Masahiro; Hagari, Shinobu; Kamahora, Toshio; Ueyama, Hiroshi; Kishi, Yosuke; Uemura, Hidetoshi; Kurimura, Takashi

doi:10.1016/0166-0934(90)90136-4

Cited by 52 publications

(16 citation statements)

References 6 publications

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“…The PCR procedures for HSV‐1 and 2 were accomplished using the reagents and protocol of the Bio‐Core HSV 1/2 PCR kit (Bio‐Core, Seoul, Korea), and by referring to the method of Goto et al 16 . Viral DNA was extracted using the method of Yamada et al 17 . and the PCR was carried out according to the method of Matsumoto et al 18 .…”

Section: Methodsmentioning

confidence: 99%

“…The PCR procedures for HSV-1 and 2 were accomplished using the reagents and protocol of the Bio-Core HSV 1/2 PCR kit (Bio-Core, Seoul, Korea), and by referring to the method of Goto et al 16 Viral DNA was extracted using the method of Yamada et al 17 and the PCR was carried out according to the method of Matsumoto et al 18 A set of primers (S, sense; AS, antisense) (IS, 5¢-CAGGGGTATAAGGACATCCA-3¢; IAS, 5¢-GG-GTCCTCGTCCAGATCGCT-3¢) were employed to amplify the region encompassing the BamHI B fragment (the 5¢ end) of the HSV-1 sequence. Another set of primers (IIS, 5¢-GCCT-CTTTTCCCCCGGGGAG-3¢; IIAS, 5¢-GGGAAAAAAGCCGCGCGG-GG-3¢) were used to amplify the region just downstream of the a¢ sequence (the 5¢ end) of the HSV-2 sequence.…”

Section: Detection Of Herpes Simplex Virusmentioning

confidence: 99%

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Characteristics of cutaneous cytomegalovirus infection in non-acquired immune deficiency syndrome, immunocompromised patients

et al. 2006

British Journal of Dermatology

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Section: Methodsmentioning

confidence: 99%

Section: Detection Of Herpes Simplex Virusmentioning

confidence: 99%

Characteristics of cutaneous cytomegalovirus infection in non-acquired immune deficiency syndrome, immunocompromised patients

et al. 2006

British Journal of Dermatology

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“…Then, by controlling the pH and the salinity of the medium it is possible to extract nucleic acids from any complex medium and to release them in small volume which leads to purification and concentration processes by using cationic magnetic particles [2]. The extracted nucleic acid molecules can then be amplified on the magnetic beads or after desorption step and removal of the magnetic particles using PCR (polymerase chain reaction) [36] in the case of DNA molecule or RT-PCR (Reverse Transcriptase PCR) [37] in the case of RNA as below illustrated [38].…”

Section: Nucleic Acids Extraction Concentration and Detectionmentioning

confidence: 99%

Advances in the biomedical applications of reactive colloids

Elaı̈ssari¹,

Fessi²

2009

Braz. J. Phys.

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This short review aimed to give to reader's brief applications of polymer colloids in biomedical area such as therapy and medical diagnosis as also developed in our laboratory. Indeed, the polymer particles and composite particles are commonly used in immunoassays as solid phase supports, for the encapsulation of active agents and for the immobilization of biomolecules such as oligonucleotides, proteins or antibodies. In the area of composite particles, magnetic particles bearing immobilized biomolecules are used in biomedical diagnosis such as immunoassay, specific nucleic acids concentration, cell labelling and separation and in numerous biotechnological applications.

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“…Method 2 [2] : 200 µL guanidine isothiocynate and 20 µL glass powder were added to 200 µL serum and incubated at ambient temperature for 90 min and centrifuged at 12 000 g. The supernatant was removed and the pellet was washed and dissolved in reverse transcription buffer PCR detection.…”

Section: Extraction Of Virus Nucleic Acidsmentioning

confidence: 99%

Simultaneous detection of HBV and HCV by multiplex PCR normalization

Wang

Huard

Han

2004

WJG

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AIM:To design and establish a method of multiplex PCR normalization for simultaneously detecting HBV and HCV. METHODS:Two pairs of primers with a 20 bp joint sequence were used to amplify the target genes of HBV and HCV by two rounds of amplification. After the two rounds of amplification all the products had the joint sequence. Then the joint sequence was used as primers to finish the last amplification. Finally multiplex PCR was normalized to a single PCR system to eliminate multiplex factor interference. Four kinds of nucleic acid extraction methods were compared and screened. A multiplex PCR normalization method was established and optimized by orthogonal design of 6 key factors. Then twenty serum samples were detected to evaluate the validity and authenticity of this method. RESULTS:The sensitivity, specificity, diagnostic index and efficiency were 83.3%, 70%, 153.3% and 72.2%, respectively for both HBsAg and anti-HCV positive patients, and were 78.6%, 80%, 258.6% and 79.2%, respectively for HBsAg positive patients, and were 75%, 90%, 165% and 83.3%, respectively for anti-HCV positive patients. CONCLUSION:The multiplex PCR normalization method shows a broad prospect in simultaneous amplification of multiple genes of different sources. It is practical, correct and authentic, and can be used to prevent and control HBV and HCV.Wang N, Gao XQ, Han JX. Simultaneous detection of HBV and HCV by multiplex PCR normalization.

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A new method for extracting DNA or RNA for polymerase chain reaction

Cited by 52 publications

References 6 publications

Characteristics of cutaneous cytomegalovirus infection in non-acquired immune deficiency syndrome, immunocompromised patients

Characteristics of cutaneous cytomegalovirus infection in non-acquired immune deficiency syndrome, immunocompromised patients

Advances in the biomedical applications of reactive colloids

Simultaneous detection of HBV and HCV by multiplex PCR normalization

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