Fiber and vessel structures located in the cross-section are anatomical features that play an important role in identifying tree species. In order to determine the microscopic anatomical structure of these cell types, each cell must be accurately segmented. In this study, a segmentation method is proposed for wood cell images based on deep convolutional neural networks. The network, which was developed by combining two-stage CNN structures, was trained using the Adam optimization algorithm. For evaluation, the method was compared with SegNet and U-Net architectures, trained with the same dataset. The losses in these models trained were compared using IoU (Intersection over Union), accuracy, and BF-score measurements on the test data. The automatic identification of the cells in the wood images obtained using a microscope will provide a fast, inexpensive, and reliable tool for those working in this field.