A new method for the quantitative analysis of endodontic microleakage

Haïkel, Youssef; Wittenmeyer, William; Bateman, Geoffrey; Bentaleb, A.; Allemann, Claude

doi:10.1016/s0099-2399(99)80136-8

Cited by 93 publications

(70 citation statements)

References 16 publications

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“…In vitro에서 충전된 근관의 밀폐효과를 평가하기 위한 미 세누출 측정방법은 색소침투법 10,11) , 방사선동위원소측정법 12) , 세균이나 세균의 대사산물을 이용한 미세누출 측정법 13) , 전기화학적 미세누출법 14) 등이 있다. 이번 연구에서는 색소 를 이용해 침투 정도를 측정하는 방법을 사용하였고, 그 과 정이 단순하고 용이하며 눈으로 직접 관찰할 수 있다는 장 점을 가지고 있다 15,16) .…”

Section: ⅳ 총괄 및 고안unclassified

Microleakage of resilon by methacrylate-based sealer and self-adhesive resin cement

Ham

Kim

Shin

et al. 2008

J Korean Acad Conserv Dent

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The purpose of this study was to compare the apical microleakage in root canal filled with Resilon by methacrylate-based root canal sealer or 2 different self-adhesive resin cements. Seventy single-rooted extracted human teeth were sectioned at the CEJ perpendicular to the long axis of the roots with diamond disk. Canal preparation was performed with crown-down technique using Profile NiTi rotary instruments and GG drill. Each canal was prepared to ISO size 40, .04 taper and 1 ㎜ short from the apex. The prepared roots were randomly divided into 4 experimental groups of 15 roots each and 5 roots each for positive and negative control group. The root canals were filled by lateral condensation as follows. Group 1: Guttapercha with AH-26, Group 2: Resilon with RealSeal primer & sealer, Group 3: Resilon with Rely-X Unicem, Group 4: Resilon with BisCem. After stored in 37℃, 100% humidity chamber for 7 days, the roots were coated with 2 layers of nail varnish except apical 3 ㎜. The roots were then immersed in 1% methylene blue dye for 7 days. Apical microleakage was measured by a maximum length of linear dye penetration after roots were separated longitudinally. One way ANOVA and Scheffe' s post-hoc test were performed for statistical analysis. Group 1 showed the least apical leakage and there was no statistical significance between Group 2, 3, 4. According to the results, the self adhesive resin cement is possible to use as sealer instead of primer & sealant when root canal filled by Resilon. [J Kor Acad Cons Dent 33(3):204-212, 2008]

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Section: ⅳ 총괄 및 고안unclassified

Microleakage of resilon by methacrylate-based sealer and self-adhesive resin cement

Ham

Kim

Shin

et al. 2008

J Korean Acad Conserv Dent

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“…자가산부식 전처리제를 적용한 후에 상아질은 건조되어야 하는데, 전처 리제가 물이나 ethanol, acetone 등의 용매를 포함하고 있 기 때문이며 29) , 용매의 잔존은 접착제의 중합에 부정적인 영 향을 줄 수 있다고 보고된 바 있다 30) . 31,32) , 방사선동위 원소측정법 33) , 세균이나 세균의 대사산물을 이용한 미세누 출 측정법 34) , 전기화학적 미세누출법 35) 등의 여러 가지 방법 이 있다. 그 중에서 methylene blue를 이용한 색소침투법 은 다른 염색용액에 비해 분자량이 작아서 근관내로 더 쉽 게 침투된다고 알려져 있으며 36) butyric acid와 같은 세균 의 대사산물 크기와 유사하기 때문에 미세누출 실험에 가장 많이 사용된다 37) .…”

Section: ⅰ 서 론unclassified

Microleakage of resilon: Effects of several self-etching primer

Jong-Hyeon

Shin

Cho

et al. 2008

J Korean Acad Conserv Dent

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The purpose of this study was to compare the apical microleakage in root canal filled with Resilon by several self-etching primers and methacrylate-based root canal sealer. Seventy single-rooted human teeth were used in this study. The canals were instrumented by a crown-down manner with Gate-Glidden drills and .04 Taper Profile to ISO #40. The teeth were randomly divided into four experimental groups of 15 teeth each according to root canal filling material and self-etching primers and two control groups (positive and negative) of 5 teeth each as follows: group 1 -gutta percha and AH26 � sealer; group 2 -Resilon, RealSeal ™ primer and RealSeal ™ sealer; group 3 -Resilon, Clearfil SE Bond � primer and RealSeal ™ sealer group 4 -Resilon, AdheSe � primer and RealSeal ™ sealer. Apical leakage was measured by a maximum length of linear dye penetration of roots sectioned longitudinally by diamond disk. Statistical analysis was performed using the One-way ANOVA followed by Scheffe' s test. There were no statistical differences in the mean apical dye penetration among the groups 2, 3 and 4 of self-etching primers. And group 1, 2 and 3 had also no statistical difference in apical dye penetration. But, there was statistical difference between group 1 and 4 (p < 0.05). The group 1 showed the least dye penetration. According to the results of this study, Resilon with selfetching primer was not sealed root canal better than gutta precha with AH26 � at sealing root canals. And there was no significant difference in apical leakage among the three self-etching primers. [J Kor Acad Cons Dent 33(2):133-140, 2008]

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“…1 When new sealing materials or techniques emerge, studies should be performed to warrant their clinical application and to accurately assess their characteristics and properties, particularly their sealing ability. Several methods aimed at detecting leakage have already been described in the literature, such as the conductometric technique, 2 fluid filtration, 3,4 radioisotopes, 5 bacterial penetration, 6 the electrochemical test, 7 and the use of dyes. 8,9 However, all these methods have yielded controversial results, and have therefore been widely criticized.…”

Section: Introductionmentioning

confidence: 99%

Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage

et al. 2015

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The objective of this study was to describe a new method for the quantitative analysis of a microleakage of endodontic filling materials. Forty extracted single-rooted teeth were randomly divided into three experimental groups. After root canal shaping, the experimental groups were filled using the lateral condensation technique with the Epiphany system (G1), with gutta-percha + Sealapex (G2), and with gutta-percha + AH Plus (G3). Each root was mounted on a modified leakage testing device, and caffeine solution was used as a tracer (2000 ng mL -1 , pH 6.0), applied in the coronal direction towards the tooth apex, creating a hydrostatic pressure of 2.55 kPa. Presence of caffeine in the receiving solution was measured after 10, 30, and 60 days, using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). None of the groups presented microleakage at 10 days. At 30 days, G2 and G3 showed similar infiltration patterns (means: 16.0 and 13.9 ng mL -1 , respectively), whereas G1 showed significantly higher values (mean: 105.2 ng mL -1 ). At 60 days, leakage values were 182.6 ng mL -1 for G1, 139.0 ng mL -1 for G2, and 53.5 ng mL -1 for G3. AH Plus showed the best sealing ability and HPLC-MS/MS showed high sensitivity and specificity for tracer quantification.

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A new method for the quantitative analysis of endodontic microleakage

Cited by 93 publications

References 16 publications

Microleakage of resilon by methacrylate-based sealer and self-adhesive resin cement

Microleakage of resilon by methacrylate-based sealer and self-adhesive resin cement

Microleakage of resilon: Effects of several self-etching primer

Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage

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