Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage

Michelotto, André Luiz da Costa; Gasparetto, João Cleverson; Campos, Francinete Ramos; Sydney, Gilson Blitzkow; Pontarolo, Roberto

doi:10.1590/1807-3107bor-2015.vol29.0100

Cited by 2 publications

(3 citation statements)

References 31 publications

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“…Caffeine microleakage is another method with the use of a modified dual-chamber model; epoxy resin is used for sealing of the margins instead of cyanoacrylate glue and leaked caffeine content can be detected by high-performance liquid chromatograp-hy-tandem mass spectrometry (HPLC-MS/MS) (83) or a visible ultraviolet spectrophotometer (UV-VIS detectors) (77,84). The caffeine detection limit was 2000 ng mL-1, using UV-VIS, and 10 ng mL-1, using HPLC-MS/MS.…”

Section: Resultsmentioning

confidence: 99%

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Importance and methodologies of endodontic microleakage studies: A systematic review

Jafari

2017

J Clin Exp Dent

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IntroductionIt is very important to obtain a tight seal in obturated root canal, making it necessary to conduct clinical or laboratory studies on the sealability of endodontic materials. Different methodologies have been historically used to assess microleakage of different endodontic materials. The aim of the present study was to comprehensively review different material testing methods used in microleakage studies, their interpretation and importance in endodontic literature.Material and MethodsA systematic search was conducted in Medline, Cochrane, and Web of Science databases. In addition, the reference lists of review articles on the topic were searched. No language restriction was applied. Two independent reviewers screened the article.ResultsMicroleakage is considered the single most important risk factor responsible for apical periodontitis. Dye penetration, dye diffusion, bacterial and endototoxin infiltration, fluid filtration, glucose, caffeine and protein infiltration, radioisotope penetration, animal studies, and electrochemical or 3D evaluation are different methodologies used to assess dental leakage. 91 out of 177 articles in the primary search were included in the study. These methods are very divergent in their viewpoints; that is why their results cannot be easily compared. It is necessary to standardize microleakage detection methods in order to more correctly evaluate the phenomena that are found between the root canal wall and the root canal filling materials.ConclusionsAll the methods are useful if studies are performed strictly with large sample sizes and proper control groups and if the technique can be standardized. Furthermore, more evaluations of the reliability of the methods are strongly recommended. Key words:Dental leakage, review, root canal, material testing methods, data interpretation.

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Section: Resultsmentioning

confidence: 99%

“…The caffeine detection limit was 2000 ng mL-1, using UV-VIS, and 10 ng mL-1, using HPLC-MS/MS. HPLC-MS/MS can detect concentrations at least 1000 times lower (83). …”

Section: Resultsmentioning

confidence: 99%

Importance and methodologies of endodontic microleakage studies: A systematic review

Jafari

2017

J Clin Exp Dent

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“…Even in the Iranian population, 95.4% BS in the RNs and CC in mandibular canines was noted [ 22 ]. In a Turkish subset, the BS of RNs and CNs for mandibular anterior teeth were 96–100% and 90–95% [ 32 ]. In this analysis, mandibular canines showed a high BS for the RNs (97.8%), CNs (74.8%), and CCs (67.77%).…”

Section: Discussionmentioning

confidence: 99%

Root Canal Numbers and Configurations in 1080 Permanent Canine Teeth in 270 Saudi Subjects Using Cone-Beam Computed Tomography

Shaiban,

Zayed,

Abdullah

et al. 2023

Med Sci Monit

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Background An understanding of root canal morphology is vital for successful endodontic treatment. However, variations in the root canal system of permanent canines, especially in relation to population-based diversity, are not well-documented. This study thus aimed to analyze the root canal numbers, configurations, and bilateral symmetry in 1080 permanent canine teeth among 270 Saudi individuals using cone-beam computed tomography (CBCT), contributing to the existing body of knowledge and aiding clinicians in devising effective treatment strategies. Material/Methods The CBCT images of 270 participants, encompassing 1080 canines (540 pairs of upper and lower canines), were scrutinized for root and canal counts. Canal configurations were assessed based on Ahmed’s and Vertucci’s classifications. Bilateral symmetry in these parameters was recorded and the data were statistically analyzed. Results The study revealed variable prevalence of multiple roots and canals in maxillary and mandibular canines. Ahmed’s and Vertucci’s type I canal configuration was predominantly observed. Notably, significant bilateral symmetry was noted in root and canal numbers, and canal configurations. Conclusions The most common configuration of permanent canines was a single root and canal, usually adhering to Ahmed’s and Vertucci’s type I classification. Mandibular canines showed a higher incidence of two canals than two roots. The extent of bilateral symmetry, especially in mandibular canines, could provide valuable insights for better contralateral tooth treatment planning.

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Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage

Cited by 2 publications

References 31 publications

Importance and methodologies of endodontic microleakage studies: A systematic review

Importance and methodologies of endodontic microleakage studies: A systematic review

Root Canal Numbers and Configurations in 1080 Permanent Canine Teeth in 270 Saudi Subjects Using Cone-Beam Computed Tomography

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