A New Method for the Detection of the Plasminogen Activator Content of Vein Walls

Johnson, Ralph H.; Mansfield, Averil O.

doi:10.1159/000207720

Acta Haematol

1978

DOI: 10.1159/000207720

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A New Method for the Detection of the Plasminogen Activator Content of Vein Walls

Ralph H. Johnson¹,

Averil O. Mansfield²

Abstract: The plasminogen activator content of a vein wall has been measured by estimating the radio-activity released from a ¹²⁵I-tagged fibrin clot by incubating that clot with a known weight of the vein wall. This method could be used in the investigation of the fibrinolytic system of the body in its normal distribution and in diseases of the vascular system.

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1993

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Cited by 6 publications

References 5 publications

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The fibrinolytic activity of the vein following venous stasis

Mansfield

1979

Journal of British Surgery

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This animal study investigated the fibrinolytic activity in the vein wall of the pig following venous stasis, which was induced artificially using an intermittently inflated pneumatic cuff. The method used to measure the fibrinolytic activity was modified from Todd's original technique. The area of lysis and the area of the vein wall were measured and expressed as a ratio, called the fibrinolytic index. After 3 h of intermittent venous stasis the fibrinolytic index was reduced by 68 per cent, significantly lower than the control group. The reduction of the fibrinolytic index by venous stasis could play an important part in the development of deep vein thrombosis.

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The fibrinolytic activity of the vein following venous stasis

Mansfield

1979

Journal of British Surgery

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Thromboembolic complications after arthroscopy of the knee

Poulsen¹,

Borris²,

Lassen³

1993

Arthroscopy: The Journal of Arthroscopic & Related Surgery

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Isolation of Tissue Plasminogen Activator from Skin Lesions with Allergic Vasculitis

Toki¹,

Tsushima²,

Yamasaki³

et al. 1982

Journal of Investigative Dermatology

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A tissue plasminogen activator was extracted from skin lesions with allergic vasculitis and purified by successive column chromatography on Sephadex G-200, DEAE-cellulose, Hydroxyaptite-cellulose and polyacrylamide gel electrophoresis. By these procedures, 160 micrograms of enzyme with a specific activity of 843.8 international units/mg protein was obtained from 5 g of original skin. The purified material was homogeneous as ascertained by sodium dodecyl sulfate polyacrylamide gel electrophoresis and had an apparent molecular weight of 110,000 as measured by gel filtration on Sephadex G-200. Its identity with human urokinase was investigated and was found to possess the same plasminogen activator activity as that of urokinase. It had high amindolytic activity, but only slight N-alpha-acetyl-glycyl-L-lysine methyl ester esterolytic activity. This tissue plasminogen activator was confirmed to be immunologically identical to human urokinase.

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A New Method for the Detection of the Plasminogen Activator Content of Vein Walls

Cited by 6 publications

References 5 publications

The fibrinolytic activity of the vein following venous stasis

The fibrinolytic activity of the vein following venous stasis

Thromboembolic complications after arthroscopy of the knee

Isolation of Tissue Plasminogen Activator from Skin Lesions with Allergic Vasculitis

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