A New Method for Vital Staining of Central Nervous Tissues With Neutral Red

Maeda, Ryuei; Ihara, Nobuo; Takada, Reiko; Kondo, Tadashi; Nakano, Emyo; Yamato, Toshiko

doi:10.1111/j.1440-1827.1971.tb00116.x

Cited by 1 publication

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rat was killed with ketamine i.v., and the brain was removed. Neutral red is an intravital dye 12 that stains normally perfused brain tissue intensely red. The dye was injected to determine whether tissue receiving blood through collateral vessels was less intensely stained than tissue receiving blood through normal routes.…”

Section: Experimental Protocolmentioning

confidence: 99%

“…12 Occlusion of the middle cerebral artery (MCA) increases the diameter of these anastomoses 34 and, in 5-week-old normotensive Wistar-Kyoto rats (WKY), occlusion of the MCA does not produce infarction. 3 In contrast to WKY, in strokeprone spontaneously hypertensive rats (SHRSP), major cerebral vessels are smaller in lumenal diameter, 6 cerebral vascular resistance is greater, 7 and occlusion of the MCA produces infarction.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Blood flow through cerebral collateral vessels one month after middle cerebral artery occlusion.

Coyle¹,

Heistad²

1987

Stroke

View full text Add to dashboard Cite

Acute occlusion of a middle cerebral artery (MCA) reduces cerebral blood flow in normotensive Wistar-Kyoto rats (WKY) and in stroke-prone spontaneously hypertensive rats (SHRSP). The goal of this study was to determine whether MCA occlusion produces a sustained reduction in cerebral blood flow or whether collateral vessels restore blood flow to normal levels. We measured blood flow through cerebral collateral vessels to the territory of the occluded MCA and to homologous tissue of the other hemisphere in WKY 1 month after occlusion of the MCA. Cerebral blood flow, measured with mkrospheres, was restored to normal levels under control conditions in the territory of the occluded MCA. During vasodilatation produced by seizures, blood flow and vascular conductance were increased to similar levels in tissue distal to the site of MCA occlusion and in the homologous tissue of the other hemisphere. MCA occlusion did not produce infarction in any of the WKY. In contrast, 1 month after MCA occlusion in SHRSP, a large atrophic infarct was invariably present in the territory of the occluded MCA. The number of collateral vessels to the territory of the MCA do not differ in SHRSP and WKY. Internal diameter and orientation of the anastomotic vessels differ in SHRSP and WKY. We conclude that, after 1 month of MCA occlusion, changes in the collateral vessels supplying the territory of the occluded MCA in WKY were sufficient to restore blood flow to normal under control conditions and to virtually normal levels during vasodilatation. We suggest that, in normotensive rats, protection against infarction after occlusion of the MCA may relate, at least in part, to effective dilator reserve in collateral vessels. (Stroke 1987;18:407-411) D STAL branches of the 3 major cerebral arteries anastomose to form an extensive collateral network in rats.12 Occlusion of the middle cerebral artery (MCA) increases the diameter of these anastomoses 34 and, in 5-week-old normotensive Wistar-Kyoto rats (WKY), occlusion of the MCA does not produce infarction.3 In contrast to WKY, in strokeprone spontaneously hypertensive rats (SHRSP), major cerebral vessels are smaller in lumenal diameter, 6 cerebral vascular resistance is greater, 7 and occlusion of the MCA produces infarction. 3Five minutes after MCA occlusion, blood flow through collateral vessels to the territory of the occluded MCA is significantly less in SHRSP than in WKY.8 Furthermore, vasodilatation produced by seizures after MCA occlusion produces minimal increases in blood flow to the territory of the occluded MCA in SHRSP and WKY. If the anastomoses in Received March 21, 1986; accepted October 14, 1986. WKY enlarge progressively after occlusion of the MCA, 34 blood flow through the collateral vessels should increase toward normal levels and if sufficient, protect tissue distal to the occlusion from infarction.In this study, we measured blood flow through cerebral collateral vessels in WKY after 1 month of MCA occlusion. Flow was measured during control conditions and during vasodila...

show abstract

Section: Experimental Protocolmentioning

confidence: 99%

mentioning

confidence: 99%