A new methodology of mesenchymal stem cell expansion using magnetic nanoparticles

Incorporation of Capillary-Like Structures into Dermal Cell Sheets Constructed by Magnetic Force-Based Tissue Engineering

“…We previously reported that MCLs do not adversely affect proliferation of several types of normal cells within the range of MCL concentrations tested (i.e., human aortic endothelial cells [HAECs], <100 pg-magnetite/cell (Ito et al, 2004c); human keratinocytes, <50 pg/cell (Ito et al, 2004a); human smooth muscle cells, <100 pg-magnetite/cell (Ito et al, 2005b); human mesenchymal stem cells [MSCs], <100 pg/cell (Ito et al, 2004b)), and we observed no effects on MSC differentiation (Ito et al, 2004b). However, further study is needed in order to assess the toxicity of residual magnetic nanopartilces in the grafts before Mag-TE can be used clinically.…”

Nanotechnology in Drug Delivery

“…Liposomes are also studied as carriers for cells, genes or DNA fragments. Ito et al (65) studied the effect of magnetite cationic liposomes which have positive surface charge to enrich and proliferate Mesenchymal stem cells (MSCs) in vitro. Kunisawa et al (66) established a protocol for the encapsulation of nanoparticles in liposomes, which were further fused with ultra violet-inactivated Sendai virus to compose fusogenic liposomes and observed that fusogenic liposome demonstrated a high ability to deliver nanoparticles containing DNA into cytoplasm.…”

Novel Promises of Nanotechnology for Tissue Regeneration

“…Mesenchymal stem cells incubated in osteogenic medium with these nanoparticles changed their shape from fibroblastic to polygonal, formed calcium nodules and increased in number five-fold compared with controls (Ito et al, 2004). In addition, superparamagnetic iron oxide nanoparticles have been shown to enhance the survival rate of stem cells up to 99%, indicating that these nanoparticles improve stem cell viability (Delcroix et al, 2009).…”