A new micellar aqueous two-phase partitioning system (ATPS) for the separation of proteins

Kresheck, Gordon C.; Wang, Zheng

doi:10.1016/j.jchromb.2007.08.037

Cited by 17 publications

(3 citation statements)

References 24 publications

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“…Therefore, twophase aqueous complex fluid systems can instead be used, since both phases are predominately compromised of water. Due to the mild nature of these solvents, proteins, viruses, and bacteria have already been separated in these systems (Albertsson, 1989;Albertsson et al, 1987;Capezio et al, 2005;Elling et al, 1991;Kamei et al, 2002a;Kresheck and Wang, 2007;Kula, 1990;Li and Peeples, 2004;Liu et al, 1998;Morre and Morre, 2000;Quina and Hinze, 1999;Rabelo et al, 2004;Rangel-Yagui et al, 2003). Moreover, other advantages of these systems include low cost compared to chromatography (Champluvier and Kula, 1992;Lindblom, 1983;Lindblom et al, 1983) and the ability to concentrate a biomolecule by varying the relative volumes of the two phases (Johansson and Reczey, 1998).…”

Section: Introductionmentioning

confidence: 99%

Concentration of mammalian genomic DNA using two‐phase aqueous micellar systems

Mashayekhi

Meyer

Shiigi

et al. 2008

Biotech & Bioengineering

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The concentration of biomarkers, such as DNA, prior to a subsequent detection step may facilitate the early detection of cancer, which could significantly increase chances for survival. In this study, the partitioning behavior of mammalian genomic DNA fragments in a two-phase aqueous micellar system was investigated using both experiment and theory. The micellar system was generated using the nonionic surfactant Triton X-114 and phosphate-buffered saline (PBS). Partition coefficients were measured under a variety of conditions and compared with our theoretical predictions. With this comparison, we demonstrated that the partitioning behavior of DNA fragments in this system is primarily driven by repulsive, steric, excluded-volume interactions that operate between the micelles and the DNA fragments, but is limited by the entrainment of micelle-poor, DNA-rich domains in the macroscopic micelle-rich phase. Furthermore, the volume ratio, that is, the volume of the top, micelle-poor phase divided by that of the bottom, micelle-rich phase, was manipulated to concentrate DNA fragments in the top phase. Specifically, by decreasing the volume ratio from 1 to 1/10, we demonstrated proof-of-principle that the concentration of DNA fragments in the top phase could be increased two- to nine-fold in a predictive manner.

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Section: Introductionmentioning

confidence: 99%

Concentration of mammalian genomic DNA using two‐phase aqueous micellar systems

Mashayekhi

Meyer

Shiigi

et al. 2008

Biotech & Bioengineering

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“…Aqueous two-phase systems (ATPS) are a liquid–liquid extraction technique that has been used in the separation of a wide range of products including proteins, , enzymes, , pigments, , bioproducts, , among others. The simplicity of the required equipment and mild operating conditions make this technique attractive for recovering biotechnological products .…”

Section: Introductionmentioning

confidence: 99%

Liquid–Liquid Equilibrium of Dipotassium Hydrogen Phosphate/Sodium Dihydrogen Phosphate + Butane-1,4-diol + Water Systems: Experimental Measurement and Correlation

Caballero-Sanchez,

Lázaro-Mixteco,

Vargas-Tah

et al. 2024

J. Chem. Eng. Data

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The liquid−liquid equilibrium of the ternary systems dipotassium hydrogen phosphate (K 2 HPO 4 )/sodium dihydrogen phosphate (NaH 2 PO 4 ) + butane-1,4-diol (BDO) + water was determined at T = (298.15, 308.15, and 318.15) K. The salting-out effect of the tie lines of the aqueous two-phase systems (ATPS) was evaluated, and it was found that K 2 HPO 4 had a larger salting-out coefficient, indicating that it has greater ability in phase separation because it presented a larger biphasic area in the ternary diagram. The electrolyte nonrandom two-liquid thermodynamic model was used to correlate the experimental data of both systems at different temperatures. It was found that the partition coefficients and BDO recovery are favored for both systems when the molality of the salts in the initial ATPS mixture increases. K 2 HPO 4 presented the highest values of the ATPS, a partition coefficient of 1219.83, a recovery of 95.78%, and a purity of 87.60% mass, under conditions close to salt saturation; therefore, a process can be designed to recover BDO that is produced by the biotechnological route because BDO can be concentrated from dilute aqueous solutions.

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“…Other two phase systems reported in the literature are cloud point systems or micellar ATPS, composed of surfactants in aqueous solutions at a concentration that forms a surfactant‐rich phase (coacervate phase) and a dilute phase. These systems have been applied to separate proteins in extractive fermentations for lipase, β‐lactoglobulin, and proteins embedded in the membrane . Micellar ATPS are suitable for the recovery of hydrophobic or amphiphilic products, due to the dual nature of the molecule and the wide variety of surfactants available exhibiting different solubility.…”

Section: Introductionmentioning

confidence: 99%

An integrated process for thein siturecovery of prodigiosin using micellar ATPS from a culture ofSerratia marcescens

Chávez-Castilla

Aguilar

2016

J. Chem. Technol. Biotechnol.

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BACKGROUND In situ product recovery (ISPR), or extractive fermentation, is a strategy for the primary recovery of metabolites. A micellar aqueous two‐phase system (ATPS) composed of a surfactant‐rich phase was coupled to batch and continuous fermentations for the production and in situ recovery of the red alkaloid prodigiosin from Serratia marcescens. A cloud point ATPS was formed at 24 °C using triton X‐114 and used as a production/extraction system for this metabolite, which shows therapeutic properties as antibiotic, anti‐proliferative and anti‐malarial activities. RESULTS The integration of the micellar ATPS in batch fermentation resulted in the production of 342 mg prodigiosin L−1 with a recovery of 81% in the extractive phase. When a continuous fermentation was performed, based on a cloud point system, 1.2‐fold prodigiosin content was obtained compared with batch mode and a recovery of 88% of the metabolite. This continuous operation resulted in 0.43 g of purified prodigiosin from a 1.5 L bioreactor with a concentration of 872 mg prodigiosin L−1 of extractive phase and a purity of 82%, demonstrating particular selectivity of the system for prodigiosin as a primary recovery method. This is the first report on the continuous production of this generally recognized secondary metabolite. CONCLUSION The experimental approach proposed proved that an ISPR system could lead to the establishment of a more efficient process for continuous large‐scale prodigiosin production. The proposal of a primary recovery strategy with only one bioprocess step enhances this metabolite production by lowering the inhibiting properties of prodigiosin for the microorganism. © 2016 Society of Chemical Industry

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A new micellar aqueous two-phase partitioning system (ATPS) for the separation of proteins

Cited by 17 publications

References 24 publications

Concentration of mammalian genomic DNA using two‐phase aqueous micellar systems

Concentration of mammalian genomic DNA using two‐phase aqueous micellar systems

Liquid–Liquid Equilibrium of Dipotassium Hydrogen Phosphate/Sodium Dihydrogen Phosphate + Butane-1,4-diol + Water Systems: Experimental Measurement and Correlation

An integrated process for thein siturecovery of prodigiosin using micellar ATPS from a culture ofSerratia marcescens

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