A New Microsporidian Infecting the Musculature of the Endangered Tidewater Goby (Gobiidae)

McGourty, Katie R.; Kinziger, Andrew P.; Hendrickson, G. L.; Goldsmith, Greg; Casal, Graça; Azevedo, Carlos

doi:10.1645/ge-994r.1

Cited by 18 publications

(16 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In cross sections, the coils appeared as a pair of beaded extensions along the two inner sides of the spore wall. The same observation was reported by Sprague (1966), Rodriguez-Tovar et al (2003), andMcGourty et al (2007). Numerous macroscopic, black cysts (xenomas), ranging in size from 3 to 5 mm, were observed throughout the abdominal cavity of the infected fish being embedded in different organs.…”

Section: Discussionsupporting

confidence: 85%

Microsporidian parasites: a danger facing marine fishes of the Red Sea

et al. 2010

View full text Add to dashboard Cite

Out of 600 marine fish from the Red Sea belonging to three different species that were collected and examined for microsporidian parasites, 87 (14.5%) fish were found to be infected. The infection was recorded as cysts or xenomas embedded in the gut epithelium and the peritoneal cavity of the three fish species. The highest percent of infection with microsporidian parasites was recorded in Saurida tumbil 19.5% (39/200) followed by Pagrus pagrus 15% (45/300) and the lowest percent of infection was recorded in Epinephelus chlorostigma 3% (three out of 100). After rupture of the cysts, the spores were released and examined by light microscopy. Each spore was elongated to ellipsoidal in shape and possessed a posterior vacuole which is characteristic to phylum Microspora. They measure 1.6 ± 0.5 μm (1.5-2.4 μm) × 1.3 ± 0.1 μm (1.3-2.0 μm) in Saurida tumbil and Pagrus pagrus, respectively. The spores of Pleistophora sp recorded from E. chlorostigma were ovoid to pyriform in shape and measure 1.9 ± 0.5 μm (1.8-2.7 μm) × 1.6 ± 0.4 μm (1.5-2.4 μm).

show abstract

Section: Discussionsupporting

confidence: 85%

Microsporidian parasites: a danger facing marine fishes of the Red Sea

et al. 2010

View full text Add to dashboard Cite

show abstract

“…In cross sections, the coils appeared as a pair of beaded extensions along the two inner sides of the spore wall. The same observation was reported by Sprague (1966)), Rodriguez-Tovar et al (2003), andMcGourty et al (2007). Inside the SPV wall, the plasmodia divide stepwise into uninucleate sporoblasts, which finally mature into spores as recorded by Lorn and Dykova (1992)).…”

Section: Discussionsupporting

confidence: 81%

Morphological and molecular biological characterization of Pleistophora aegyptiaca sp. nov. infecting the Red Sea fish Saurida tumbil

et al. 2011

View full text Add to dashboard Cite

One hundred three out of 225 (45.8%) of the Red Sea fish Saurida tumbil were infected with microsporidian parasites. The infection was recorded as tumor-like masses (whitish macroscopic cysts) or xenomas often up to 2 cm in diameter and embedded in the peritoneal cavity. Generally, the infection was increased during winter 63.8% (86 out of 135) and fall to 18.9% (17 out of 90) in summer. Light microscopic study revealed that xenomas were encapsulated by a fibrous layer encircling numerous sporophorous vesicles filled with mature spores measuring 1.7 ± 0.6 (1.5-2.7 μm) × 1.5 ± 0.3 μm (1.2-1.8 μm) in size. Ultrastructural microscopic study showed the presence of smooth membranes of the sarcoplasmic reticulum forming a thick, amorphous coat surrounding various developmental stages of the parasite. The various recognizable stages of the parasite were uninuclear, binucleated, and multinucleated meronts followed by detachment of the plasmalemma of the sporont from the sporophorous vesicle producing sporoblasts. Mature spores consist of a spore coat and spore contents. The spore contents consist of the uninucleated sporoplasm and a posterior vacuole located at the posterior end. The polar tube consists of a straight shaft and a coiled region (26-32 coils) arranged in many rows along the inside periphery of the spore. The polaroplast consisted of an anterior region of closely and loosely packed membranes. Molecular analysis based on the small subunit rDNA gene was performed to determine the phylogenetic position of the present species. The percentage identity between this species and a range of other microsporidia predominantly from aquatic hosts demonstrated a high degree of similarity (>92%) with eight Pleistophora species. Comparison of the nucleotide sequences and divergence showed that the sequence of the present microsporidium was most similar to that of Pleistophora anguillarum (99.8% identity) differing in 13 nucleotide positions. So, the present species was recorded and phylogenetically positioned as a new species of Pleistophora.

show abstract

“…For some genera, such as Amazonspora (Azevedo and Matos 2003), Kabatana (Lom et al 1999(Lom et al , 2001McGourty et al 2007) and Spraguea (Loubès et al 1979;Takvorian and Cali 1986;Freeman et al 2004) the fingerprint-like structures of the external surface of the exospore wall are a morphological characteristic common at all species. Usually, the external ornamentation is regularly distributed and the elevations on the surface of the mature spores, when they are observed in tangential section, present a hexagonal fingerprint-like shape (genus Amazonspora) or a tubular shape (genera Kabatana and Spraguea).…”

Section: Discussionmentioning

confidence: 99%

Redefining the genus Spraguea based on ultrastructural and phylogenetic data from Spraguea gastrophysus n. sp. (Phylum Microsporidia), a parasite found in Lophius gastrophysus (Teleostei) from Brazil

et al. 2012

Self Cite

View full text Add to dashboard Cite

The ultrastructure of the fish-infecting microsporidium Spraguea gastrophysus found in the dorsal ganglia and kidney of the anglerfish, Lophius gastrophysus (family Lophiidae) collected on the Brazilian Atlantic coast is described. Each whitish xenoma (up to 3.1 × 1.8 mm) contains several groups of parasites. The host cells are hypertrophied and contain various parasite life stages including mature spores and several developmental stages with unpaired nuclei. Monomorphic spores are ellipsoidal, lightly curved and measure about 3.35 × 1.71 μm. The spore contains a gradually tapering isofilar polar filament with five to six coils arranged in a single row. The nucleus occupies a central zone of the sporoplasm where also several polyribosomes are presented. The posterior vacuole contains a voluminous spherical and granular posterosome measuring up to ~0.65 μm in diameter. The partial small subunit, intergenic spacer and partial large subunit rRNA gene were sequenced and the phylogenetic analysis places the microsporidian described here in the clade that includes all sequences of the Spraguea genus. The ultrastructural morphology of the xenoma and the spores of this microsporidian parasite, as well as the molecular and phylogenetic analysis, suggest the description of a new species. A redefining of the genus Spraguea is also done.

show abstract

A New Microsporidian Infecting the Musculature of the Endangered Tidewater Goby (Gobiidae)

Cited by 18 publications

References 15 publications

Microsporidian parasites: a danger facing marine fishes of the Red Sea

Microsporidian parasites: a danger facing marine fishes of the Red Sea

Morphological and molecular biological characterization of Pleistophora aegyptiaca sp. nov. infecting the Red Sea fish Saurida tumbil

Redefining the genus Spraguea based on ultrastructural and phylogenetic data from Spraguea gastrophysus n. sp. (Phylum Microsporidia), a parasite found in Lophius gastrophysus (Teleostei) from Brazil

Contact Info

Product

Resources

About