A New Model Trypanosomatid,
            <i>Novymonas esmeraldas</i>
            : Genomic Perception of Its “
            <i>Candidatus</i>
            Pandoraea novymonadis” Endosymbiont

Zakharova, Alexandra; Saura, Andreu; Butenko, Anzhelika; Podešvová, Lucie; Warmusová, Sandra; Kostygov, Alexei Y.; Nenarokova, Anna; Lukeš, Julius; Opperdoes, Frederik; Yurchenko, Vyacheslav

doi:10.1128/mbio.01606-21

Cited by 9 publications

(8 citation statements)

References 106 publications

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“…Moreover, our knockdown analysis indicates that in terms of successful kDNA segregation and cell cycle completion, TbNAB70 may have rendered TbLAP1 partially redundant. Based on our survey of gene conservation, Novymonas esmeraldas represents the ideal organism to investigate this hypothesis, having lost both ‘recent’ additions of TbNAB70 and TAP110 , combined with its recently established tractability to investigate specific genes of interest (50,51). Coincidently, we also note that two of three surveyed organisms which have specifically lost TbNAB70 and/or TAP110 , also bear bacterial endosymbionts, endosymbiotic acquisitions representing a comparatively rare occurrence among trypanosomatids (52).…”

Section: Discussionmentioning

confidence: 99%

A novel nabelschnur protein regulates segregation of the kinetoplast DNA inTrypanosoma brucei

Cadena,

Hammond,

Tesařová

et al. 2024

Preprint

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The kinetoplast DNA (kDNA), a distinctive arrangement of mitochondrial DNA found in trypanosomatid protists, comprises a concatenated network of minicircles and maxicircles that undergo division and segregation once during each cell cycle. Despite the identification and characterization of numerous proteins involved in kDNA maintenance and replication, its segregation and the formation of the nabelschnur remain poorly understood on a molecular level. This enigmatic filamentous structure, transiently appearing in Trypanosoma brucei, connects the daughter kDNA networks prior to their complete segregation. Here, we characterize TbNAB70, a high mobility group box-like protein localized exclusively to the nabelschnur and the kDNA disc. Our findings demonstrate that TbNAB70 is critical for the segregation, but not replication, of kDNA, a so far unprecedented phenotype. Furthermore, structural predictions suggest that this protein holds the capacity to bind to kDNA illuminating the exact molecular mechanisms of segregation involved. Thus, we propose that TbNAB70 plays a pivotal role in the faithful and efficient segregation of the daughter kDNA networks.

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Section: Discussionmentioning

confidence: 99%

A novel nabelschnur protein regulates segregation of the kinetoplast DNA inTrypanosoma brucei

Cadena,

Hammond,

Tesařová

et al. 2024

Preprint

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“…The species identity was confirmed by diagnostic PCR and sequencing as in [80]. Total DNA and RNA was isolated and sequenced as described previously [81] at Macrogen Europe (Amsterdam, the Netherlands) and Institute of Applied Biotechnologies (Prague, Czech Republic). The TruSeq DNA PCR-free protocol was used for genomic DNA library; it was sequenced on NovaSeq 6000 resulting in ~55M 150 bp paired-end reads.…”

Section: Cultivation Total Dna and Rna Isolation And Next-generation ...mentioning

confidence: 99%

“…Populations were selected in liquid M199 medium supplemented with an additional 50 μg/ml Hygromycin and 100 μg/ml Neomycin (InvivoGen, San Diego, USA). Deletion of gene of interest was confirmed by PCR, RT-qPCR and Southern blotting as in [81]. The gene add-back was done via integration of the HA 3 -tagged target gene in the 18S rRNA locus as described previously [118].…”

Section: Establishment Of Crispr-cas9 System and Genetic Manipulation...mentioning

confidence: 99%

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Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content

et al. 2022

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Leishmaniasis is a parasitic vector-borne disease caused by the protistan flagellates of the genus Leishmania. Leishmania (Viannia) guyanensis is one of the most common causative agents of the American tegumentary leishmaniasis. It has previously been shown that L. guyanensis strains that carry the endosymbiotic Leishmania RNA virus 1 (LRV1) cause more severe form of the disease in a mouse model than those that do not. The presence of the virus was implicated into the parasite’s replication and spreading. In this respect, studying the molecular mechanisms of cellular control of viral infection is of great medical importance. Here, we report ~30.5 Mb high-quality genome assembly of the LRV1-positive L. guyanensis M4147. This strain was turned into a model by establishing the CRISPR-Cas9 system and ablating the gene encoding phosphatidate phosphatase 2-like (PAP2L) protein. The orthologue of this gene is conspicuously absent from the genome of an unusual member of the family Trypanosomatidae, Vickermania ingenoplastis, a species with mostly bi-flagellated cells. Our analysis of the PAP2L-null L. guyanensis showed an increase in the number of cells strikingly resembling the bi-flagellated V. ingenoplastis, likely as a result of the disruption of the cell cycle, significant accumulation of phosphatidic acid, and increased virulence compared to the wild type cells.

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“…The genome of the endosymbiont-bearing Novymonas esmeraldas, the closest known relative of dixenous Leishmaniinae, revealed a very similar gene content to the latter with the large number of GP63 proteases and pteridin/biopterin transporters, recognized virulence factors of Leishmania spp. Owing to the presence of the endosymbiont, this flagellate became prototrophic for all amino acids, heme, and most vitamins, i.e., even more independent of the presence of essential nutrients in the host than Strigomonadinae [12,96].…”

Section: Monoxenous Leishmaniinaementioning

confidence: 99%

Genomics of Trypanosomatidae: Where We Stand and What Needs to Be Done?

2021

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Trypanosomatids are easy to cultivate and they are (in many cases) amenable to genetic manipulation. Genome sequencing has become a standard tool routinely used in the study of these flagellates. In this review, we summarize the current state of the field and our vision of what needs to be done in order to achieve a more comprehensive picture of trypanosomatid evolution. This will also help to illuminate the lineage-specific proteins and pathways, which can be used as potential targets in treating diseases caused by these parasites.

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A New Model Trypanosomatid, Novymonas esmeraldas : Genomic Perception of Its “ Candidatus Pandoraea novymonadis” Endosymbiont

Cited by 9 publications

References 106 publications

A novel nabelschnur protein regulates segregation of the kinetoplast DNA inTrypanosoma brucei

A novel nabelschnur protein regulates segregation of the kinetoplast DNA inTrypanosoma brucei

Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content

Genomics of Trypanosomatidae: Where We Stand and What Needs to Be Done?

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