A new monoterpenoid glycoside and a new phenolic glycoside isolated from <i>Dracocephalum moldavica</i> and their anti-complementary activity

et al. 2022

Polymers

This investigation focuses on the three novel polysaccharides from Cordyceps militaris and then discusses their characterization and anti-complementary activity. The three polysaccharides from C. militaris (CMP-1, CMP-2 and CMP-3) were prepared using a DEAE-52 cellulose column. The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation. The evaluation of anti-complementary activity demonstrated that the three polysaccharides significantly inhibited complement activation through the classical pathway and alternative pathway. Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system. The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides. These results suggested that the three novel polysaccharides are potential candidates for anti-complementary drugs.

Section: Methodsmentioning

confidence: 99%

Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris

Wang

et al. 2022

Polymers

“…The activity of complement activation was detected by erythrocyte hemolysis test as previously described (Li et al 2022;Wang et al 2021). The CP assay was conducted in a total volume of 300 µL using normal human serum pool (NHSP) (1:70) in GVB ++ as a complement source and sensitized sheep erythrocytes (EAs, 2.0 × 10 9 cells/mL).…”

Section: Anti-complementary Assaymentioning

confidence: 99%

A new megastigmane glycoside, a new organic acid glycoside and other constituents with anticomplementary activity from Artemisia halodendron

Natural Product Research

Zhou

et al. 2022

Self Cite

A new megastigmane glycoside, (1R,5R,6S,7E)-megastigman-3,9-dione-7-en-6,11-diol 11-O-β-D-glucopyranoside (1), and a new organic acid glycoside, methyl (4R)-4-O-β-D-glucopyranosyl-decanoate (2), together with eight known compounds (3-10), were isolated from the aerial parts of Artemisia halodendron Turcz. ex Bess. (Asteraceae). Their chemical structures were elucidated by 1D and 2D NMR and HR-ESI-MS spectra and DP4+ probability analysis. Among the identified compounds, compounds 5, 6 and 10 were isolated from the family Asteraceae, and compounds 3, 4 and 7-9 were identified from the genus Artemisia for the first time. All of the compounds were evaluated for their anticomplementary activity against the classical pathway (CP) and the alternative pathway (AP). Compounds 7 and 9 showed anticomplementary activity with the CH 50 values of 0.31 ± 0.08 and 0.50 ± 0.09 mM, respectively.

“…Previous studies have reported that the aerial part of D. moldavica contains flavonoids, lignans, phenols, terpenoids and polysaccharides, [16] and has been used for the treatment of fever, headache, sore throat, asthma, and dysentery [17] . Our previous studies have isolated and screened several small molecular compounds with anti‐complementary activity from D. moldavica , [18–20] whereas the isolation, characterization and anti‐complementary activity of its polysaccharide macromolecules have rarely been reported.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Structural Characterization of Two Polysaccharides from Dracocephalum moldavica and Their Anti‐Complementary Activity

Wang

Chemistry & Biodiversity

et al. 2022

Self Cite

The two novel polysaccharides, DMP‐1 and DMP‐2, with molecular weights of 4.1553×105 kDa and 1.9764×105 kDa, respectively, were isolated from Dracocephalum moldavica. The structural characterization indicated that DMP‐1 and DMP‐2 shared a similar backbone consisting of →5)‐Araf‐(1→, Manp‐(1→, Glcp‐(1→, →2)‐Manp‐(1→, →6)‐Glcp‐(1→ and →3,6)‐Galp‐(1→ with a different molar ratios and triple‐helix structures with α‐ and β‐type glycosidic bonds. The anti‐complementary activity evaluation showed that DMP‐1 and DMP‐2 had strong complement inhibition through the classical pathway (CP), alternative pathway (AP) and lectin pathway (LP). Mechanistic studies indicated that DMP‐1 can block the activation cascade of the complement system by targeting C2, C3, C5, C9, Factor B and Factor P, and that DMP‐2 inhibited complement activation by blocking C2, C3, C4, C5, C9 and Factor B.