A new nanoPCR molecular assay for detection of porcine bocavirus

Wang, Xiaoling; Bai, Aiquan; Zhang, Jing; Kong, Miaomiao; Yong, Cui; Ma, Xingjie; Xia, Ai; Tang, Qun; Cui, Shangjin

doi:10.1016/j.jviromet.2014.02.029

Cited by 17 publications

(10 citation statements)

References 22 publications

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“…The traditional identification methods may not provide accurate distinction of the attenuated vaccine strain for the field strain. The nanoparticle-assisted RT-PCR assay has been used for rapid and accurate detection of other pig viruses [24][25][26]. The present study demonstrated that an effective nanoparticle-assisted RT-PCR assay targeting the ORF3 gene of PEDV was able to distinguish field strains from attenuated vaccine strains.…”

Section: Discussionmentioning

confidence: 66%

Establishment of a nanoparticle-assisted RT-PCR assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus

Zhu

Wang²,

Cui

et al. 2016

Arch Virol

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Porcine epidemic diarrhea virus (PEDV) can cause serious disease and even death in neonatal piglets, resulting in serious damage to the swine industry worldwide. Open reading frame 3 (ORF3) is the only accessory gene in the PEDV genome. Previous studies have indicated that PEDV vaccine strains have a partial deletion in ORF3. In this study, a nanoparticle-assisted polymerase chain reaction (nanoparticle-assisted RT-PCR) assay targeting the ORF3 of PEDV was developed to distinguish PEDV field strains from attenuated strains by using a specific pair of primers. The PCR products of field strains and attenuated strains were 264 bp and 215 bp in length, respectively. The sensitivity and specificity of this assay were also assessed. The nanoparticle-assisted RT-PCR assay was 10-100 times more sensitive than the conventional RT-PCR assay, with no cross-reactions when amplifying porcine pseudorabies virus (PRV), porcine circovirus type 2 (PCV2), classical swine fever virus (CSFV), porcine parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine rotavirus (RV), and porcine transmissible gastroenteritis virus (TGEV). The nanoparticle-assisted RT-PCR assay we describe here can be used to distinguish field strains from vaccine strains of PEDV, and it shows promise for reducing economic loss due to PEDV infection.

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Section: Discussionmentioning

confidence: 66%

Establishment of a nanoparticle-assisted RT-PCR assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus

Zhu

Wang²,

Cui

et al. 2016

Arch Virol

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“…In addition, the nano-PCR assay does not require specialized instruments beyond standard PCR equipment ). The nano-PCR assay has been used in detecting viral RNA molecules from animals due to its simplicity and high sensitivity including pseudorabies , porcine bocavirus (Wang et al 2014), and mink enteritis virus . In this study, a highly efficient and practical method for the detection of DTMUV was established.…”

Section: Resultsmentioning

confidence: 99%

Development of a nano-particle-assisted PCR assay for detection of duck tembusu virus

Yuan

Jianuan

Peng

et al. 2015

Lett Appl Microbiol

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“…In some research reports, nanomaterials such as carbon nanopowder [37], nano alloys [38], and multiwall carbon nanotubes [39] were successfully used to enhance and increase the efficiency of PCR. Some studies addressed an important point as to whether the nanomaterials compromised the fidelity of DNA replication in the process of PCR [40,41]. Other research reports have successfully used this technique for the diagnosis and detection of bacterial aerosols [42], porcine bocavirus [43], pseudorabies virus [44] and porcine parvovirus [45].…”

Section: Introductionmentioning

confidence: 99%

ZnO Nanoflower-Based NanoPCR as an Efficient Diagnostic Tool for Quick Diagnosis of Canine Vector-Borne Pathogens

Upadhyay

Yang²,

Zaman

et al. 2020

Pathogens

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Polymerase chain reaction (PCR) is a unique technique in molecular biology and biotechnology for amplifying target DNA strands, and is also considered as a gold standard for the diagnosis of many canine diseases as well as many other infectious diseases. However, PCR still faces many challenges and issues related to its sensitivity, specificity, efficiency, and turnaround time. To address these issues, we described the use of unique ZnO nanoflowers in PCR reaction and an efficient ZnO nanoflower-based PCR (nanoPCR) for the molecular diagnosis of canine vector-borne diseases (CVBDs). A total of 1 mM of an aqueous solution of ZnO nanoflowers incorporated in PCR showed a significant enhancement of the PCR assay with respect to its sensitivity and specificity for the diagnosis of two important CVBDs, Babesia canis vogeli and Hepatozoon canis. Interestingly, it drastically reduced the turnaround time of the PCR assay without compromising the yield of the amplified DNA, which can be of benefit for veterinary practitioners for the improved management of diseases. This can be attributed to the favorable adsorption of ZnO nanoflowers to the DNA and thermal conductivity of ZnO nanoflowers. The unique ZnO nanoflower-assisted nanoPCR greatly improved the yield, purity, and quality of the amplified products, but the mechanism behind these properties and the effects and changes due to the different concentrations of ZnO nanoflowers in the PCR system needs to be further studied.

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A new nanoPCR molecular assay for detection of porcine bocavirus

Cited by 17 publications

References 22 publications

Establishment of a nanoparticle-assisted RT-PCR assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus

Establishment of a nanoparticle-assisted RT-PCR assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus

Development of a nano-particle-assisted PCR assay for detection of duck tembusu virus

ZnO Nanoflower-Based NanoPCR as an Efficient Diagnostic Tool for Quick Diagnosis of Canine Vector-Borne Pathogens

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