Development of a nano-particle-assisted PCR assay for detection of duck tembusu virus

Yuan, Wanzhe; Jianuan, Li; Peng, Li Na; Sun, Jianhe; Chen, Ligong; Liu, Juxiang

doi:10.1111/lam.12509

Cited by 17 publications

(11 citation statements)

References 17 publications

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“…More sensitive assay may be needed for DTMUV detection in mosquito population, for example, a nanoparticle‐assisted polymerase chain reaction (nano‐PCR) assay. This assay was developed for DTMUV detection, and it was 10‐fold more sensitive than a conventional PCR assay which the lower detection limit of this assay was 1.8 × 10 2 copies per µl (Wanzhe et al, ).…”

Section: Resultsmentioning

confidence: 99%

Duck Tembusu virus detection and characterization from mosquitoes in duck farms, Thailand

Sanisuriwong

Yurayart

Thontiravong

et al. 2020

Transbound Emerg Dis

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Duck Tembusu virus (DTMUV), an emerging infectious disease in ducks, belongs to the Flavivirus genus and Flaviviridae family. The transmission of DTUMV involves mosquito vectors; however, the exact role of mosquitoes in the ecology of DTMUV in Thailand remains unclear. This study was conducted to examine DTMUV detection and characterization from mosquitoes in duck farms in central Thailand. Mosquitoes were collected from two duck farms in Sing Buri Province and two duck farms in Ang Thong Province from September 2015 to July 2016 using four CDC-light traps. A total of 30,841 mosquitoes were collected and identified to seven species (Anopheles (An.) barbirostris, An. stephensi, Culex (Cx.) gelidus, Cx. quinquefasciatus, Cx. tritaeniorhynchus, Mansonia (Ma.) annulifera and Ma. uniformis). The most common collected species from each duck farm and each collection time was Cx. tritaeniorhynchus. Mosquitoes were pooled according to species, location, and collection time and then examined for DTMUV by RT-PCR. A total of 273 mosquito pools were examined, with only one pool of Cx. tritaeniorhynchus collected from Sing Buri Province in November 2015 testing positive for DTMUV. Phylogenetic analysis of the polyprotein genes demonstrated that a mosquito-derived Thai DTMUV was grouped into subcluster 2.1 and most closely related to the 2013 Thai DTMUVs. Thus, this study indicated that Cx. tritaeniorhynchus may play a role as a vector in the transmission of DTMUV in Thailand. However, additional studies concerning the vector competence of this mosquito for DTMUV are needed. K E Y W O R D S detection, duck farms, duck Tembusu virus, mosquito, Thailand, vector How to cite this article: Sanisuriwong J, Yurayart N, Thontiravong A, Tiawsirisup S. Duck Tembusu virus detection and characterization from mosquitoes in duck farms, Thailand.

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Section: Resultsmentioning

confidence: 99%

Duck Tembusu virus detection and characterization from mosquitoes in duck farms, Thailand

Sanisuriwong

Yurayart

Thontiravong

et al. 2020

Transbound Emerg Dis

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“…In the detection of duck Tembusu virus, the detection limit of nano-particle-assisted PCR was 1.8 × 10 2 copies [23]. This indicates that the sensitivity of nano-particleassisted PCR was 10-fold more than that of conventional PCR assay [23]. In theory, PCR assay can detect as few as 1 DNA/RNA molecule.…”

Section: Discussionmentioning

confidence: 96%

“…This indicates that RT-PCR can detect PDCoV with a detection limit of 1000 copies. Recently, nano-particle-assisted PCR assays were used to increase the sensitivity of conventional PCR [22][23][24]. In the detection of duck Tembusu virus, the detection limit of nano-particle-assisted PCR was 1.8 × 10 2 copies [23].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, nano-particle-assisted PCR assays were used to increase the sensitivity of conventional PCR [22][23][24]. In the detection of duck Tembusu virus, the detection limit of nano-particle-assisted PCR was 1.8 × 10 2 copies [23]. This indicates that the sensitivity of nano-particleassisted PCR was 10-fold more than that of conventional PCR assay [23].…”

Section: Discussionmentioning

confidence: 99%

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Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay

Zeng

Huang

et al. 2018

BioMed Research International

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Porcine deltacoronavirus (PDCoV) is a newly discovered coronavirus, which belongs to the family Coronaviridae. It causes watery diarrhea, vomiting, and dehydration in newborn piglets. A sensitive RT-PCR method is urgently required to detect PDCoV infection. In this study, we developed and evaluated a conventional RT-PCR assay and a SYBR green-based real-time RT-PCR assay that targeted the PDCoV n gene. Both assays are specific and have the same limit of detection at 2 × 101 copies of RNA molecules per reaction. Eighty-four clinical samples were subjected to both conventional RT-PCR and real-time RT-PCR, and the same positive rate (41.7%) was achieved, which was much higher than the positive rate (26.2%) using a previously described one-step RT-PCR technique. In summary, a conventional RT-PCR technique was successfully established for the detection of PDCoV with the same detection limit as a SYBR green-based real-time RT-PCR assay.

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“…Photonic nanocrystals chemically functionalized with biomolecular probes have allowed for the detection of markers of viral disease through an antigen-antibody interaction, including those of SARS-CoV [43]. Semiconductor quantum dots conjugated with an RNA oligonucleotide were also shown to be sensitive to 422 amino acid residues long SARS-CoV N protein and capable of screening for the potential inhibitors of this protein in a biochip The reader may consult the following references for more information: [37][38][39][40][41][42]. FN: False negative; FP: False positive; PCR: polymerase chain reaction; TP: True positive.…”

Section: Nanotechnologies In Coronavirus Diagnosticsmentioning

confidence: 99%

Why Have Nanotechnologies Been Underutilized in The Global Uprising Against The Coronavirus Pandemic?

Uskoković

2020

Nanomedicine (Lond.)

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Prior research on nanotechnologies in diagnostics, prevention and treatment of coronavirus infections is reviewed. Gold nanoparticles and semiconductor quantum dots in colorimetric and immunochromatographic assays, silica nanoparticles in a polymerase chain reaction and spike protein nanospheres as antigen carriers and adjuvants in vaccine formulations present notable examples in diagnostics and prevention, while uses of nanoparticles in coronavirus infection treatments have been merely sporadic. The current absence of antiviral therapeutics that specifically target human coronaviruses, including SARS-CoV-2, might be largely due to the underuse of nanotechnologies. Elucidating the interface between nanoparticles and coronaviruses is timely, but presents the only route to the rational design of precisely targeted therapeutics for coronavirus infections. Such a fundamental approach is also a viable prophylaxis against future pandemics of this type.

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Development of a nano-particle-assisted PCR assay for detection of duck tembusu virus

Cited by 17 publications

References 17 publications

Duck Tembusu virus detection and characterization from mosquitoes in duck farms, Thailand

Duck Tembusu virus detection and characterization from mosquitoes in duck farms, Thailand

Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay

Why Have Nanotechnologies Been Underutilized in The Global Uprising Against The Coronavirus Pandemic?

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