A New Neuroprotective Pinusolide Derivative from the Leaves of Biota orientalis.

Koo, Kyung Ah; Sung, Sang Hyun; Kim, Young Choong

doi:10.1248/cpb.50.834

Cited by 43 publications

(39 citation statements)

References 26 publications

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“…All other chemicals were of the highest purity available. Pinusolide and 15-MPA were isolated from the barks of B. orientalis as previously reported and their purities were higher than 95.0% (Koo et al, 2002).…”

Section: Methodsmentioning

confidence: 83%

“…The leaves of B. orientalis have been used in Oriental medicine for the treatment of gout, rheumatism, diarrhoea, and chronic tracheitis (Zhu et al, 2004). We have previously shown that the diterpenoids, pinusolide and its derivative, 15-methoxypinosolidic acid (15-MPA), isolated from B. orientalis, protect against glutamate-induced neurotoxicity (Koo et al, 2002). In the present study, we investigated the effect of pinusolide and 15-MPA on apoptosis induced by STS in primary cultures of rat cortical cells and further elucidated the mechanism of their anti-apoptotic effects.…”

Section: Introductionmentioning

confidence: 99%

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Pinusolide and 15‐methoxypinusolidic acid attenuate the neurotoxic effect of staurosporine in primary cultures of rat cortical cells

Koo

Lee

Kim

et al. 2007

British J Pharmacology

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Background and purpose: Apoptosis is a fundamental process required for neuronal development but also occurs in most of the common neurodegenerative disorders. In an attempt to obtain an anti-apoptotic neuroprotective compound from natural products, we isolated the diterpenoids, pinusolide and 15-MPA, from B. orientalis and investigated their neuroprotective activity against staurosporine (STS) -induced neuronal apoptosis. In addition, we determined the anti-apoptotic mechanism of these compounds in rat cortical cells. Experimental approach: Primary cultures of rat cortical cells injured by STS were used as an in vitro assay system. Cells were pretreated with pinusolide or 15-MPA before exposure to STS. Anti-apoptotic activities were evaluated by the measurement of cytoplasmic condensation and nuclear fragmentation. The levels of cellular peroxide, malondialdehyde (MDA) and [Ca 2 þ ] i , as well as the activities of superoxide dismutase (SOD) and caspase-3/7, were measured. Key results: Pinusolide and 15-MPA, at a concentration of 5.0 ìM, reduced the condensed nuclei and rise in [Ca 2 þ ] i that accompanies apoptosis induced by 100 nM STS. Pinusolide and 15-MPA also protected the cellular activity of SOD, an antioxidative enzyme reduced by STS insult. Furthermore, the overproduction of reactive oxygen species and lipid peroxidation induced by STS was significantly reduced in pinusolide and 15-MPA treated cells. In addition, pinusolide and 15-MPA inhibited STS-induced caspase-3/7 activation. Conclusions and Implications: These results show that pinusolide and 15-MPA protect neuronal cells from STS-induced apoptosis, probably by preventing the increase in [Ca 2 þ ] i and cellular oxidation caused by STS, and indicate that they could be used to treat neurodegenerative diseases.

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Section: Methodsmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Pinusolide and 15‐methoxypinusolidic acid attenuate the neurotoxic effect of staurosporine in primary cultures of rat cortical cells

Koo

Lee

Kim

et al. 2007

British J Pharmacology

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“…From the IR, 13 C-and distortionless enhancement by polarization transfer (DEPT) NMR spectra suggested that 1 was a diterpene containing a carboxylic acid, an a,b-unsaturated glactone, and a methyoxyl functionalities. The 1 H-NMR spectral data showed that 1 had the characteristic pattern of a labdane-type diterpene with terminal olefinic protons (d 4.54, 4.87) and two tertiary methyl protons (d 0.58, 1.22).…”

mentioning

confidence: 99%

“…The H-11 (d 1.59, 1.76) had correlation with H-20 (d 0.58) determined the configuration of methine proton H-9 to be at a-axial orientation. A compound (elucidated as 7) 13) was isolated from the leaves of Biota orientalis by Koo. The 1 H-, 13 C-NMR and other physical data of that compound and 1 are almost similar.…”

mentioning

confidence: 99%

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Two New Compounds from the Leaves of Calocedrus microlepic var. formosana

Chien

Liu

Kuo

2004

CHEMICAL & PHARMACEUTICAL BULLETIN

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