A New Option in Solid Phase Synthesis of DNA-Fragments

Solid‐Phase Supports for Oligonucleotide Synthesis

“…For example, hydrazine hydrate/pyridine/acetic acid can be used as the deprotection reagent if the N 6 -isobutyryl-protecting group on deoxyguanosine is replaced with an N 6 -(N′,N′-dibutylformamidine)-protecting group (Urdea and Horn, 1986). Base deprotection using ethanolamine can also be accomplished, but the N 4 -protection on deoxycytidine must be modified to prevent N 4 -hydroxyethylation (Berner et al, 1989). Allylic protection on all of the bases and on the phosphate linkages can also be removed using palladium reagents without affecting the succinyl linker (Hayakawa et al, 1990).…”