A New Osteoporosis Prevention Supplements-diet Improve Bone Mineral Density in Ovariectomized Rats on Micro-CT

Tanaka, Sakae; Watanabe, Tetsu; Takahashi, Toshio; Fujita, Kou; Tanimoto, Yasuhiro; Teranishi, Mari; Kato, Takao; Kawai, Yasuhiko

doi:10.2485/jhtb.23.1

Cited by 11 publications

(14 citation statements)

References 34 publications

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“…It has been reported that changes in bone induced by reduced secretion of a female hormone, estrogen, similar to those in humans, occur in experimental osteoporosis animal models 2) . Experimental ovariectomized rats (OVX) are used in compounded osteoporosis-preventive supplemental feed (High mineral diet (HMD): calcium compounding ratio 3.0%) to OVX and observed changes in bone quality 6,7) . We reported that feeding HMD to OVX for 24 weeks was useful to improve bone quality of the femoral diaphysis by increasing BMD and breaking strength 6) .…”

Section: Introductionmentioning

confidence: 99%

“…Experimental ovariectomized rats (OVX) are used in compounded osteoporosis-preventive supplemental feed (High mineral diet (HMD): calcium compounding ratio 3.0%) to OVX and observed changes in bone quality 6,7) . We reported that feeding HMD to OVX for 24 weeks was useful to improve bone quality of the femoral diaphysis by increasing BMD and breaking strength 6) . However, although the compounded calcium level, 3.0%, in HDM was useful to improve bone quality, the mineral intake became excessive for the body, and it may upset the nutrient balance 6) .…”

Section: Introductionmentioning

confidence: 99%

“…We reported that feeding HMD to OVX for 24 weeks was useful to improve bone quality of the femoral diaphysis by increasing BMD and breaking strength 6) . However, although the compounded calcium level, 3.0%, in HDM was useful to improve bone quality, the mineral intake became excessive for the body, and it may upset the nutrient balance 6) . To improve HMD, we prepared a new triple compound feed, Additive Formula Diet (AFD), in which the calcium source was changed to calcium citrate, which is more soluble than calcium phosphate 8,9) , and the calcium level was adjusted to a level similar to that in general commercial feeds (compounding ratio: 1.0 %).…”

Section: Introductionmentioning

confidence: 99%

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Bone Quality Improvement in Ovariectomized Rats Dosed with New Osteoporosis-Preventive Compound: Analyses with Raman Spectroscopy and Polarized Light Microscopy

Tanaka

Takahashi

Sato

et al. 2017

J. Hard Tissue Biology.

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In this study, to observe the influence of Additive Formula Diet (AFD) ingestion in ovariectomized rats (OVX), OVX were fed a normal mineral diet (NMD) or AFD, and structures of the outer and inner regions of the cross-section of the central region of the femoral diaphysis and changes in crystalline bone quality were investigated by polarized light microscopy and Raman spectroscopy. For experimental animals, 8 rats ovariectomized at 20 weeks old were used. OVX were divided into 2 groups: NMD-fed ONMD group and AFD-fed OAFD group, and the femur was excised 24 weeks after intervention. On polarized light microscopy, the external basic lamella was thicker in the OAFD than ONMD group, showing higher-order colors. On Raman spectroscopy, the degree of calcification and crystallinity in the inner region tended to slightly higher in the OAFD than ONMD group, although crystallinity tended to slightly lower. Ingestion of AFD may have improved bone metabolism throughout the body, and improvement of the femoral mineral levels and collagen cross linkages may have more markedly improved bone quality compared with that by NMD ingestion, suggesting its usefulness for treatment of osteoporosis, bone healing after tooth extraction, and early bone formation after implant placement.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bone Quality Improvement in Ovariectomized Rats Dosed with New Osteoporosis-Preventive Compound: Analyses with Raman Spectroscopy and Polarized Light Microscopy

Tanaka

Takahashi

Sato

et al. 2017

J. Hard Tissue Biology.

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“…Thus, in previous studies, we analyzed bone samples with micro-CT imaging and subsequently performed bending tests and sectioning, thereby permitting multiple analyses on the same sample [6][7][8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

“…We have previously conducted research involving the development of a high mineral diet (HMD) that contains fructooligosaccharide (FOS), isoflavone (ISO), and calcium citrate (CC) with calcium phosphate as an osteoporosis prevention supplement 10) . An earlier analysis of some of the results showed that HMD is beneficial for bone quality improvement, as demonstrated by the elevation in BMD and bone mineral content (BMC) of the femoral diaphysis in ovariectomized (OVX) rats after 24 weeks of the diet 10) .…”

Section: Introductionmentioning

confidence: 99%

Structure Model Index Changes in the Femoral Epiphyseal Region on Micro-Computed Tomography Caused by a Supplement Diet in Ovariectomized Rats

Tanaka

Watanabe

Takahashi

et al. 2014

J. Hard Tissue Biology.

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We have developed a novel supplement diet for osteoporosis prevention that contains fructooligosaccharide, isoflavone, and calcium citrate, in addition to calcium phosphate (high mineral diet: HMD). The present study aimed to clarify whether rats with osteoporosis fed an HMD showed an improved structure model index (SMI) in the femoral epiphyseal region compared with rats fed a normal mineral diet (NMD). The experiment used 20-week-old ovariectomized rats divided into an NMD group (n=6) and an HMD group (n=6). After 24 weeks on the diet, this study examined the changes in SMI and the 3-dimensional pseudocolor map (3D-map) using micro-computed tomography. Compared to the NMD group, the HMD group had significantly greater Trabecular Thickness (Tb. Th) and Trabecular Number, and shorter Trabecular Separation and Trabecular Spacing, indicating highly dense trabeculae. The trabeculae of the NMD group had low Tb. Th and bone volume / tissue volume (BV/TV), indicating a thin, rod-like shape, but the trabeculae of the HMD group had significantly greater Tb. Th and BV/TV, demonstrating a thick, plate-like structure. In the sagittal section image of the 3D-map, the trabecular bone of the NMD group showed low bone mineral density (BMD), represented by light blue and purple colors, on the growth plate side of the inner portion of the cortical bone, indicating near complete resorption of the trabecular bone. The trabecular bone of the HMD group showed moderate to low BMD, represented by light blue and green colors, on the growth plate side, indicating a greater trabecular bone density compared to the NMD group. The present results showed that the intake of HMD, compared to NMD, maintains the trabecular structure by preventing trabecular bone resorption, indicating the usefulness of HMD intake.

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Biobased Nanomaterials in Nutraceuticals

Ghosh,

Choudhury,

Singh

et al. 2024

Biobased Nanomaterials

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A New Osteoporosis Prevention Supplements-diet Improve Bone Mineral Density in Ovariectomized Rats on Micro-CT

Cited by 11 publications

References 34 publications

Bone Quality Improvement in Ovariectomized Rats Dosed with New Osteoporosis-Preventive Compound: Analyses with Raman Spectroscopy and Polarized Light Microscopy

Bone Quality Improvement in Ovariectomized Rats Dosed with New Osteoporosis-Preventive Compound: Analyses with Raman Spectroscopy and Polarized Light Microscopy

Structure Model Index Changes in the Femoral Epiphyseal Region on Micro-Computed Tomography Caused by a Supplement Diet in Ovariectomized Rats

Biobased Nanomaterials in Nutraceuticals

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