A new pancreatic lipase inhibitor from Broussonetia kanzinoki

Ahn, Jong Hoon; Liu, Qing; Lee, Chul; Ahn, Mi‐Jeong; Yoo, Hwan‐Soo; Hwang, Bang Yeon; Lee, Mi Kyeong

doi:10.1016/j.bmcl.2012.02.088

Cited by 44 publications

(30 citation statements)

References 16 publications

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“…Broussonone A that were isolated from the stem barks of Broussonetia kanzinoki showed a noncompetitive inhibitory activity on pancreatic lipase with an IC 50 of 28.4 µM (Ahn et al, 2012). Buthanol extract of Streptomyces variabilis strain of PO-178 produced pancreatic lipase inhibitor with IC 50 of 44.32 mg/ml (Kekuda & Onkaroppa, 2014).The study of Adnyana et al (2014) showed that ethanolic extract of pomegranate leaves inhibited pancreatic lipase with IC 50 20.64 ug/ml.…”

Section: Pancreatic Lipase Inhibitory Activity Assay Of Extracts Strementioning

confidence: 99%

Characterization of Ethanolic Extract of Streptomyces sp. As a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12

et al. 2017

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Endophytic Streptomyces sp. AEBg12 isolated from Zingiber cassumunar (Bangle) is known to produce pancreatic lipase inhibitory compound. However, the characteristics of this active compound has not been reported yet. This study aimed to determine the characteristics of pancreatics inhibitory compound produced by Streptomyces sp. AEBg12 and to assess the role of endophytic actinobacteria in producing pancreatic lipase inhibitor using endophytic-free bangle tissue culture, wild bangle and compared with the activity of Streptomyces sp. AEBg12 endophytes. Supernatant of Streptomyces sp. AEBg12 was extracted using ethanol, ethyl acetate, and n-hexane solvents. Toxicity test was performed using larvae of shrimp Artemia salina. The results showed that the best solvent to obtain pancreatic lipase inhibitor compounds was ethanol. Phytochemical analysis showed that ethanolic extract of endophytic Streptomyces sp. AEBg12 contained flavonoids. IC 50 value of ethanol extract was 180.83 µg/ml. The result of TLC showed that ethanolic extract of Streptomyces AEBg12 had a blue luminescence band indicated that there were either flavone, flavanones, flavonols or isoflavones. Inhibitory activity of Streptomyces sp. AEBg12 was higher than wild bangle and bangle tissue culture. The information from this study can be be used as a basic data for further characterization of the active compound, which might be developed as an antiobesity agent through its pancreatic lipase inhibitory activity.

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Section: Pancreatic Lipase Inhibitory Activity Assay Of Extracts Strementioning

confidence: 99%

Characterization of Ethanolic Extract of Streptomyces sp. As a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12

et al. 2017

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“…Broussonone A (1), a p-quinol natural product, was first isolated by our group from stem barks of the Korean plant Broussonetia kanzinoki Sieb (Moraceae) [1]. This plant is one of the most abundant trees in Korea, which has been extensively used as diuretic or tonic agents [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…This plant is one of the most abundant trees in Korea, which has been extensively used as diuretic or tonic agents [1][2][3][4][5]. Broussonone A (1) exhibited inhibitory activity on pancreatic lipase with IC50 of 28.4 μM.…”

Section: Introductionmentioning

confidence: 99%

Concise Synthesis of Broussonone A

Choi

Lee

et al. 2015

Molecules

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A concise and expeditious approach to the total synthesis of broussonone A, a p-quinol natural compound, has been developed. The key features of the synthesis include the Grubbs II catalyst mediated cross metathesis of two aromatic subunits, and a chemoselective oxidative dearomatizationin the presence of two phenol moieties. Especially, optimization associated with the CM reaction of ortho-alkoxystyrenes was also studied, which are known to be ineffective for Ru-catalyzed metathesis reactions under conventional reaction conditions because ortho-alkoxy group could coordinate to the ruthenium center, resulting in the potential complication of catalyst inhibition.

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“…The PL activity was evaluated using porcine PL (Ahn et al 2012). Briefly, enzyme solution was prepared by reconstituting the porcine PL (Sigma-Aldrich Corp., St. Louis, MO, USA) in 0.1 M Tris-HCl buffer (pH 8).…”

Section: Measurement Of Pl Activitymentioning

confidence: 99%

Optimization of pancreatic lipase inhibitory and antioxidant activities of Ilex paraguariensis by using response surface methodology

Shin

Jeon

et al. 2016

Arch. Pharm. Res.

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Response surface methodology (RSM) using a Box-Behnken design was used to optimize the extraction conditions for obtaining pancreatic lipase inhibitory and antioxidant principles from Ilex paraguariensis leaves. Three influencing factors: extraction time (min), the liquid-solid ratio, and ethanol concentration (%, v/v) were investigated in the ultrasonic extraction process. Optimization of the extraction conditions to obtain a product with minimum PL activity, maximum antioxidant activity, and maximum yield was performed using RSM by focusing on the three target influencing factors. The optimum conditions were established as the ethanol concentration (54.8 %), liquid-solid ratio (35.4), and extraction time (70.0 min). Under these conditions, the 2,2-diphenyl-1-picrylhydrazyl scavenging activity, PL activity, extraction yield were 59.3 ± 3.5, 35.3 ± 3.0, and 34.4 ± 0.4 %, respectively, similar to the theoretical predicted values of 59.7, 35.2, and 34.3 %, respectively.

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A new pancreatic lipase inhibitor from Broussonetia kanzinoki

Cited by 44 publications

References 16 publications

Characterization of Ethanolic Extract of Streptomyces sp. As a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12

Characterization of Ethanolic Extract of Streptomyces sp. As a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12

Concise Synthesis of Broussonone A

Optimization of pancreatic lipase inhibitory and antioxidant activities of Ilex paraguariensis by using response surface methodology

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