Characterization of Ethanolic Extract of Streptomyces sp. As a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12

Abstract

Endophytic Streptomyces sp. AEBg12 isolated from Zingiber cassumunar (Bangle) is known to produce pancreatic lipase inhibitory compound. However, the characteristics of this active compound has not been reported yet. This study aimed to determine the characteristics of pancreatics inhibitory compound produced by Streptomyces sp. AEBg12 and to assess the role of endophytic actinobacteria in producing pancreatic lipase inhibitor using endophytic-free bangle tissue culture, wild bangle and compared with the activity of Streptomyces sp. AEBg12 endophytes. Supernatant of Streptomyces sp. AEBg12 was extracted using ethanol, ethyl acetate, and n-hexane solvents. Toxicity test was performed using larvae of shrimp Artemia salina. The results showed that the best solvent to obtain pancreatic lipase inhibitor compounds was ethanol. Phytochemical analysis showed that ethanolic extract of endophytic Streptomyces sp. AEBg12 contained flavonoids. IC 50 value of ethanol extract was 180.83 µg/ml. The result of TLC showed that ethanolic extract of Streptomyces AEBg12 had a blue luminescence band indicated that there were either flavone, flavanones, flavonols or isoflavones. Inhibitory activity of Streptomyces sp. AEBg12 was higher than wild bangle and bangle tissue culture. The information from this study can be be used as a basic data for further characterization of the active compound, which might be developed as an antiobesity agent through its pancreatic lipase inhibitory activity.

Short communication: Endophytic actinobacteria isolated from ginger (Zingiber officinale) and its potential as a pancreatic lipase inhibitor and its toxicity

“…This shows that some of the ginger endophytic actinobacterial isolates have a higher ability to produce inhibitory values compared to their host plants (Figure 2). This result is in accordance with the study of pancreatic lipase inhibitors conducted by Fitri et al (2017b), where AEBg12 isolates from the bangle plant have higher pancreatic lipase inhibitors than their host plants. The resistance value of the AEBg12 isolate obtained was 95.6%.…”

“…This is based on his research which contains pancreatic lipase inhibitors in gelugur acid (Garcinia atroviridis), galangal (Alpinia galanga) and kencur (Kaempferia galanga) which produce research on flavonoids. The results of the study by Fitri et al (2017b) also showed Streptomyces sp. AEBg12 from Bangle (Zingiber cassumunar) which produces pancreatic lipase inhibitors, produces a flavonoid composition in the three extracts distributed.…”

Probability Induction of Kratom Plant Bioactive Components in Antidiabetic and Antiobesity Studies

“…AEBg12 derived from the bangle plant contains flavonoids, saponins and steroids that act as pancreatic lipase inhibitors. Saponins have also been shown to inhibit lipase activity both in vitro and in vivo, so saponin compounds, steroids and flavonoids contained in kratom leaves are also thought to have the potential to be lipase inhibitors and play a role in the treatment of obesity (Fitri et al, 2017).…”

Isolation and characterization of soil actinobacteria as cellulolytic enzyme producer from Aceh Besar, Indonesia

“…Aerial mycelium has a variety of colors, such as white, grey, red, green, blue, and purple. Fitri et al (2017), reported that isolates of endophytic actinobacteria grown on YMA medium produced grey, white, greenish grey, bluish grey, and turquoise aerial mycelium, while isolates grown on OA medium produced green, grey, white, and bluish green aerial mycelium.…”