Short communication:  Endophytic actinobacteria isolated from ginger (Zingiber officinale) and its potential as a pancreatic lipase inhibitor and its toxicity

Rahayu, Sri; Fitri, Lenni; Ismail, Yulia Sari

doi:10.13057/biodiv/d200510

Cited by 10 publications

(12 citation statements)

References 16 publications

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“…The lethal concentration (LC 50 ) of BLIS was determined via the probit analysis method with a 95% confidence interval using SPSS software. Each concentration was tested thrice, and artificial seawater was used as a negative control [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Partial Purification and Characterization of Bacteriocin-Like Inhibitory Substances Produced by Streptomyces sp. Isolated from the Gut of Chanos chanos

Kurnianto

Kusumaningrum

Lioe

et al. 2021

BioMed Research International

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Bacteriocin-like inhibitory substances (BLIS) have sparked great interest because of their promising use in food as natural antimicrobial agents. In this work, six Streptomyces isolates obtained from the gut of Chanos chanos demonstrated their ability to produce extracellular metabolites with inhibitory activity against Salmonella enterica serovar Typhimurium, Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus. Exposure of the extracellular metabolites to proteolytic enzymes (i.e., proteinase-K, trypsin, and pepsin) revealed high sensitivity and confirmed their proteinaceous nature. The metabolites were stable at high temperatures (up to 100°C for 30 min) and a wide range of pH (pH 2.0–7.0). Fractionation of the crude BLIS by filtration yielded three fractions based on molecular weight: <3 kDa, 3–10 kDa, and >10 kDa. Analysis of the antibacterial activity of these fractions showed increased specific activity, especially in the fraction with a molecular weight (MW) of <3 kDa, relative to the crude sample. The fraction with MW < 3 kDa had minimum inhibitory and bactericidal concentrations in ranges 0.04–0.62 mg·mL−1 and 0.08–1.25 mg·mL−1, respectively. This fraction also showed better temperature and pH stability compared with crude BLIS. Brine shrimp toxicity assay revealed that this fraction has moderate toxicity with a 50% lethal concentration of 226.975 μg·mL−1 (i.e., moderate toxicity) to Artemia salina. Identification of the peptide sequences of this fraction by liquid chromatography–tandem mass spectrometry yielded 130 proteins with retention times of 15.21–19.57 min. Eleven proteins with MWs of 1345.66–2908.35 Da and composed of less than 30 amino acid residues with high hydrophobicity (15.34–26.22 kcal·mol−1) appeared to be responsible for the antibacterial activity of the fraction. This study revealed the potential application of BLIS from Streptomyces, especially BLIS SCA-8, as antibacterial agents.

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Section: Methodsmentioning

confidence: 99%

Partial Purification and Characterization of Bacteriocin-Like Inhibitory Substances Produced by Streptomyces sp. Isolated from the Gut of Chanos chanos

Kurnianto

Kusumaningrum

Lioe

et al. 2021

BioMed Research International

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“…SM1P was higher than that of ethanol extract Streptomyces sp. AJ4 (LC50 value =653.38 μg/mL) (Rahayu, Fitri, & Ismail, 2019) but lower than n-butanol extract Streptomyces sp. (LC50 value = 42.11 μg/mL) (Prashith, Shobha, & Onkarappa, 2011).…”

Section: Resultsmentioning

confidence: 89%

Antibacterial Activities and Toxicity of Streptosporangium sp. SM1P

Nofiani

Rizky

Brilliantoro

2021

Molekul

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This study aims to explore the anti-bacterial and toxicity activities from a rare actinobacterium isolated from mangrove, Mempawah District, West Kalimantan. The mangrove mud sample from Mempawah district was inoculated on ISP4 agar using a pour plate method. After 4 days of incubation, a colony of suspected actinobacterium was appeared, then isolated and coded as SM1P. SM1P was characterized based on morphological and biochemical traits and identified as a genus of Streptroporangium then called Streptroporangium sp. SM1P. Streptroporangium sp. SM1P was carried out anti-bacterial assay on both ISP1 agar and ISP4 agar media using the cross-streak method for the solid-state fermentation. The result showed that Streptroporangium sp. SM1P could inhibit Streptococcus sp. and Salmonella typhi on ISP1 agar and treptococcus sp., Escherichia coli, Vibrio cholerae, Staphylococcus aureus and Salmonella typhi on ISP4 agar. Streptroporangium sp. SM1P was cultivated on ISP1 broth and extracted using ethyl acetate, then evaporated to obtain crude extract. The crude extract was used for anti-bacterial assay (well-diffusion method for liquid-state fermentation) and toxicity assay (brine shrimp lethality test). The crude extract was active against 2 of the test bacteria (Streptococcus sp. and E. coli). The best medium and state fermentation for anti-bacterial assay were ISP4 agar with the condition of solid-state fermentation. The extract SM1P prepared on ISP1 broth showed toxic activity based on LC50 (106.094 µg/mL). Therefore, Streptroporangium sp. SM1P have a potential source to explore secondary metabolites having anti-bacterial and toxicity activities.

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“…A ginger endophytic actinobacterial strain produced secondary metabolites with promising pancreatic lipase inhibitory activity as the percentage inhibition (≈ 90%) was significantly higher than ginger extract (≈ 69%) and standard LI (orlistat), which had approximate inhibition rate of 88% [101]. The significant lipase inhibitory activity displayed by the endophytic bacterial strain may be linked to the presence of 'terpenoids, phenols, tannins, flavonoids, alkaloids, and saponins' in the endophyte [101]. Similarly, cytosporone B and dothiorelone A, isolated from an endophytic fungus, Phomopsis sp.…”

Section: Sources Of Novel Lipase Inhibitors (Lis)mentioning

confidence: 99%

Therapeutic potentials of endophytes for healthcare sustainability

Falade

Adewole

Ekundayo

2021

Egyptian Journal of Basic and Applied Sciences

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Morbidity and mortality rates are on the upward trajectory globally, probably due to lack of effective treatments and poor healthcare. Most effective drugs are, however, characterized by serious side effects upon long usage. It is, therefore, imperative to explore natural sources for efficient therapeutics with little or no side effects. This paper outlines the therapeutic potentials of endophytes using published articles on endophytes in both Web of Science (WoS) and Scopus (1990Scopus ( -2020. Scientific evidences discussed in this review suggest endophytic microbes as reservoirs of novel bioactive compounds belonging to the following classes alkaloid, xanthones, methoxyphenols, depsipeptide, bicyclic lactones, depsidoenes, butenolides, maleimide-bearing compounds, ergosterol, spirobisnaphthalenes, benzopyran derivatives, isofuranonaphthalenone, butyrolactones, diketopiperazine, sesquiterpenoids, cytochalasin-related compounds, pestalols and cyclic pentapeptides. The identified compounds are characterized by promising therapeutic potentials such as antioxidant, anti-inflammatory, antimicrobial, anticancer, antidiabetic, antiviral, neuroprotective, and hepatoprotective properties, which are significant to healthy living and sustainable healthcare. This review further discusses the emerging potentials of endophytes in the production of antibiofilm, antimultiresistant Staphylococcus aureus (anti-MRSA) and lipase inhibitors (LIs). The prospective applications of endophytes in the development of anti-COVID-19 medications and therapeutics for the management of neglected tropical diseases (NTDs) are also advocated in this review. The therapeutic potentials of endophytes, if properly harnessed, would in no small measure contribute to good health, which is an integral part of the sustainable development goals (SDGs) of the United Nations (UN).

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Short communication: Endophytic actinobacteria isolated from ginger (Zingiber officinale) and its potential as a pancreatic lipase inhibitor and its toxicity

Cited by 10 publications

References 16 publications

Partial Purification and Characterization of Bacteriocin-Like Inhibitory Substances Produced by Streptomyces sp. Isolated from the Gut of Chanos chanos

Partial Purification and Characterization of Bacteriocin-Like Inhibitory Substances Produced by Streptomyces sp. Isolated from the Gut of Chanos chanos

Antibacterial Activities and Toxicity of Streptosporangium sp. SM1P

Therapeutic potentials of endophytes for healthcare sustainability

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