A new parameter to simultaneously assess antioxidant activity for multiple phenolic compounds present in food products

Hong, Yang; Xue, Xuejia; Li, Huan; Tay-Chan, Su Chin; Ong, Seng Poon; Tian, Enze

doi:10.1016/j.foodchem.2017.02.022

Cited by 3 publications

(1 citation statement)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, the rate constant decreases in aprotic solvents and increases in alcohols, demonstrating that a main electron-transfer mechanism is involved rather than H-atom transfer [9]. Several new and alternative approaches have been revised in recent years, underlining the drawbacks of the lack of a standardized method [10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

A Novel Stoichio-Kinetic Model for the DPPH• Assay: The Importance of the Side Reaction and Application to Complex Mixtures

et al. 2021

View full text Add to dashboard Cite

The 2,2-diphenyl-1-picrylhydrazyl (DPPH•) assay is widely used to determine the antioxidant activity of food products and extracts. However, the common DPPH• protocol uses a two-point measurement and does not give information about the kinetics of the reaction. A novel stoichio-kinetic model applied in this study monitors the consumption of DPPH• by common antioxidants following the second order reaction. The fitting of such decay yields the rate constant k1, which describes the main reaction between antioxidants and DPPH•, and the rate constant k2, which is attributed to a slower side reaction considering the products generated between the transient radicals (AO•) and another molecule of DPPH•. The model was first applied to antioxidant standards. Sinapic acid, Trolox and ascorbic and chlorogenic acids did not show any side reaction. Instead gallic, ferulic and caffeic acids achieved the best fitting with k2. The products of the side reaction for these compounds were confirmed and identified with high-resolution mass spectrometry. Finally, the kinetic model was applied to evaluate the antioxidant activity of eight herbal extracts. This study suggests a new kinetic approach to standardize the common DPPH• assay for the determination of antioxidant activity.

show abstract

Section: Introductionmentioning

confidence: 99%

A Novel Stoichio-Kinetic Model for the DPPH• Assay: The Importance of the Side Reaction and Application to Complex Mixtures

et al. 2021

View full text Add to dashboard Cite

show abstract

A novel electrochemical biosensor for antioxidant evaluation of phloretin based on cell-alginate/ʟ-cysteine/gold nanoparticle-modified glassy carbon electrode

et al. 2018

Biosensors and Bioelectronics

View full text Add to dashboard Cite

The relative antioxidant activity and steric structure of green tea catechins – A kinetic approach

Hong

Xue

et al. 2018

Food Chemistry

View full text Add to dashboard Cite

A new parameter to simultaneously assess antioxidant activity for multiple phenolic compounds present in food products

Cited by 3 publications

References 31 publications

A Novel Stoichio-Kinetic Model for the DPPH• Assay: The Importance of the Side Reaction and Application to Complex Mixtures

A Novel Stoichio-Kinetic Model for the DPPH• Assay: The Importance of the Side Reaction and Application to Complex Mixtures

A novel electrochemical biosensor for antioxidant evaluation of phloretin based on cell-alginate/ʟ-cysteine/gold nanoparticle-modified glassy carbon electrode

The relative antioxidant activity and steric structure of green tea catechins – A kinetic approach

Contact Info

Product

Resources

About