A new potyvirus first isolated and identified from Angelica sinensis

Abstract

A filamentous virus was isolated in Angelica sinensis (Angelica sinensis (Oliv.) Diels) which shows mosaic symptoms on leaves in Minxian, Gansu province, China. According to morphology and molecular biology properties, this virus, which has a flexuous rod-shaped particle about 750 nm in length and 12 nm in width, was assigned to the genus Potyvirus, family Potyviridae. Its coat protein (CP) shows high similarity with six other potyviruses by analysis of peptide mass fingerprinting (PMF). The 919 bp nucleotides of 3' terminal covering partial CP gene and 3'-untranslated region was amplified by RT-PCR using degenerate primers which were designed according to the result of PMF. In sequence comparisons and phylogenetic analysis, the new isolate was found to be closely related to Japanese hornwort mosaic virus (JHMV), Konjak mosaic virus (KoMV), and Zantedeschia mosaic virus (ZaMV). The most closely related virus is JHMV03 (AB251346), with 96.59% aa and 87.60% nt identity to the isolate. All results suggest the presence of a new member of potyvirus, tentatively named Dang Gui strain of Japanese hornwort mosaic virus (JHMV-DG*). In our research the antiserum against the CP of JHMV-DG had also been prepared. To our knowledge, it is the first time that a potyvirus has been isolated and identified in Angelica sinensis.

Rapid visual detection of Japanese hornwort mosaic virus infecting Angelica sinensis by reverse transcription loop‐mediated isothermal amplification

“…The conserved regions of CP gene were used as the basis for the design of the RT-LAMP primers for JHMV detection. This was performed by establishing sequence alignments (multiple sequence alignment ) of CP gene sequences of JHMV from A. sinensis hosts available in GenBank (accession numbers EF157843) (Zhang et al, 2009). Primer design was conducted using Primer Explorer version 5 software (Eiken Chemical Co., Ltd, Japan) with the default settings.…”

“…JHMV particles are flexuous and rod-shaped; they are approximately 750 nm in length and 12 nm in width. The coat protein (CP) gene of JHMV comprises 795 nucleotides and encodes 264 amino acids with a molecular weight of 30 kDa (Zhang et al, 2009). The host range of JHMV is restricted to Umbelliferae (Zhang et al, 2009).…”

“…The coat protein (CP) gene of JHMV comprises 795 nucleotides and encodes 264 amino acids with a molecular weight of 30 kDa (Zhang et al, 2009). The host range of JHMV is restricted to Umbelliferae (Zhang et al, 2009). Plants infected with JHMV show symptoms of plant stunting, leaf mosaic, mottling, chlorosis, distortion and necrosis of the leaf margin (Figure 1).…”

“…This approach requires visual surveillance and confirmatory diagnosis using rapid, specific and sensitive diagnostic methods. Currently, methods used for detecting JHMV in angelica include transmission electron microscopy (TEM) and reverse transcription (RT)-polymerase chain reaction (PCR) (Zhang et al, 2009). These methods are used for identifying latent infections at most stages of plant growth.…”

Expression, purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate

“…Virus infection caused serious reduction in production of Lanzhou lily and other economic corps in recent years [1,2]. Lily symptomless virus (LSV; family, Genus Carlavirus , species) is the most prevalent virus infecting Lanzhou lily [2], and it has been reported in USA, Europe, Australia and Asia [3-7].…”