Expression, purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate

Abstract

BackgroundLily symptomless virus (LSV) is widespread in many countries where lily are grown or planted, and causes severe economic losses in terms of quantity and quality of flower and bulb production. To study the structure-function relationship of coat protein (CP) of LSV, to investigate antigenic relationships between coat protein subunits or intact virons, and to prepare specific antibodies against LSV, substantial amounts of CP protein are needed.ResultsThus, full-length cDNA of LSV coat protein was synthesized and amplified by RT-PCR from RNA isolated from LSV Lanzhou isolate. The extended 33.6 kDa CP was cloned and expressed prokaryoticly and then purified by Ni-ion affinity chromatography. Its identity and antigenicity of recombinant CP were identified on Western-blotting by using the prepared anti-LSV antibodies.ConclusionsThe results indicate that fusion CP maintains its native antigenicity and specificity, providing a good source of antigen in preparation of LSV related antibodies. Detailed structural analysis of a pure recombinant CP should allow a better understanding of its role in cell attachment and LSV tropism. This investigation to LSV should provide some specific antibodies and aid to development a detection system for LSV diagnostics and epidemiologic surveys.

In vitro study of the growth, development and pathogenicity responses of Fusarium oxysporum to phthalic acid, an autotoxin from Lanzhou lily

“…unicolor Cotton), an important edible crop bulb that is mostly distributed in the middle area of Gansu Province in China (Wang et al 2010), often experiences soil sickness or replanting problems that cause a severe decrease in the yield and quality of lily bulbs. Fusarium wilt of lily is caused by pathogenic fungi, especially Fusarium species (Shang et al 2014).…”

Preliminary report on the study of postharvest fruit rot bacteria and yeasts in Lanzhou Lily (Lilium davidii var. unicolor) in China

“…In some root crops and brassicas, viral infections present before harvest can sometimes develop more rapidly after harvest. However, in general, viruses are not a common cause of postharvest disease, but they can cause severe losses in terms of quantity and quality in bulb and flower production (Wang et al, ).…”

Development of an Indirect ELISA and Immunocapture RT-PCR for Lily Virus Detection

“…In addition, contamination problems are possible during virus purification because lilies are often infected simultaneously by two or three different viruses in the field. The use of recombinant CP expression in prokaryotic systems overcomes this problem because it is simple, fast, abundant, inexpensive, and easy [2,8,15].…”

“…Bacterial expression systems are stable, have high productivity, and offer easy purification. Therefore, the plant viral CPs expressed in these systems have been reported for several plant viruses [8,15]. Use of this technique overcomes purification difficulties of virus preparations of virus-specific antisera.…”