A new procedure for quantification of lignin in soybean (Glycine max (L.) Merrill) seed coat and their relationship with the resistance to mechanical damage

Capeleti, Ines; Ferrarese, M. L. L.; Krzyzanowski, F. C.; Ferrarese-Filho, Osvaldo

doi:10.15258/sst.2005.33.2.25

Cited by 39 publications

(35 citation statements)

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“…A matéria seca obtida foi triturada, homogeneizada e submetida a sucessivas centrifugações (3300 rpm por quatro minutos) com diferentes soluções (tampão fosfato de sódio e potássio/Triton X-100/NaCl 1,0 M/acetona) para a obtenção da parede celular. A quantificação da lignina foi realizada pelo método ácido linotioglicólico -LTGA (CAPELETI et al, 2005).…”

Section: Methodsunclassified

Seleção De Linhagens De Feijão Com Base No Padrão E Na Qualidade De Sementes

et al. 2015

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RESUMO -A seleção de linhagens de feijão pelo padrão de tegumento, qualidade sanitária e fisiológica de sementes é recente nos programas de melhoramento. Por isso, foram objetivos deste trabalho avaliar se as linhagens de feijão diferem quanto ao padrão de tegumento, a qualidade sanitária e fisiológica de sementes, investigar a associação entre esses caracteres e selecionar linhagens de feijão superiores para a qualidade de sementes. Para tanto, foram avaliadas 16 linhagens de feijão de diferentes obtentores em cultivo de safra agrícola. As linhagens de feijão apresentaram diferença significativa em relação ao peso de mil sementes, coloração do tegumento, conteúdo de lignina, comprimento, largura e espessura das sementes, incidência de Fusarium sp., germinação, primeira contagem de germinação, envelhecimento acelerado, comprimento da parte aérea, da raiz e total das plântulas, massa seca da parte aérea e total das plântulas e emergência de plântulas a campo. A estimativa de correlação positiva foi observada entre a emergência de plântulas a campo e a germinação (r = 0,650), a primeira contagem de germinação (r = 0,600), o envelhecimento acelerado (r = 0,793) e a condutividade elétrica (r = 0,635). A seleção indireta pela germinação, primeira contagem de germinação e envelhecimento acelerado foi eficiente para a identificação de linhagens de feijão com maior emergência de plântulas a campo. A seleção das linhagens TB 02-07, SM 1810, Carioca, LP 08-90, SM 1107, LP 07-80, Pérola, CNFP 10104 e TB 02-24 é recomendada para uso em programas de hibridação controlada para o desenvolvimento de novas cultivares de feijão de alta qualidade de sementes. Palavras-chave: Phaseolus vulgaris L.. Características morfológicas. Qualidade sanitária. Qualidade fisiológica. Variabilidade genética. SELECTION OF COMMON BEAN LINES FOR STANDARD AND QUALITY SEEDSABSTRACT -The selection of common bean lines for seed coat standard, sanitary and physiological quality seed is recent in breeding programs. The objectives of this research were to evaluate if common bean lines differ for seed coat standard, sanitary and physiological quality seed, to investigate the correlation between these characters and to select common bean lines with high seed quality. For this, 16 inbred common bean lines were evaluated in normal rainy growing season. The common bean lines showed significant difference for the weight of thousand seeds, seed coat color, lignin content, length, width and thickness seeds, Fusarium sp. incidence, germination, first germination count, accelerated ageing, length of shoot, root and total of seedling, dry matter of shoot and total of seedling and emergency of seedling in field. A positive correlation coefficient was found between emergency of seedling in field and germination (r = 0.650), first germination count (r = 0.600), accelerated ageing (r = 0.793) and electric conductivity (r = 0.635). The indirect selection by germination, first germination count and accelerated ageing is effective to identify common bean lines with the hig...

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Section: Methodsunclassified

Seleção De Linhagens De Feijão Com Base No Padrão E Na Qualidade De Sementes

et al. 2015

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“…The result was expressed as the percentage of viability, vigor and mechanical damage (at levels 1-8) according to França Neto et al (1998). Complementarily, we quantified lignin content in the seed coat as proposed by Capeleti et al (2005), with modifications. In laboratory, four replicates were used for all tests with 50 seeds for each block.…”

Section: Methodsmentioning

confidence: 99%

“…In the cultivar Celeste, with the lowest lignin content, the incidence of mechanical damage in seeds was always among the lowest. Nevertheless, regarding the relationship between lignin content in the seed coat and resistance to mechanical damage, Capeleti et al (2005) found a direct relationship between these two characteristics, suggesting that lignin content in the seed coat above 0.4 g% may be a reasonable indicator of resistance to mechanical damage, but they estimated this resistance by means of the pendulum test, this test proposed by Carbonell and Krzyzanowski (1995).…”

Section: Lignin Content In the Seed Coatmentioning

confidence: 99%

“…This can affect the resistance to mechanical damage and physiological quality of soybean seeds, considering their relationships with the lignin content in the seed coat, as reported by Capeleti et al (2005), Panobianco et al (1999) and Santos et al (2007). There are also reports that glyphosate-resistant transgenic soybean plants could be less efficient in the accumulation of Mn than conventional soybean, so they would be more responsive to the use of this micronutrient (Gordon, 2007), but the results are still inconclusive and conflicting as to productivity (Andrade and Rosolem, 2011;Basso et al, 2011;Loecker et al, 2010;Zobiole et al, 2010).…”

Section: Introductionmentioning

confidence: 96%

“…Among the nutrients, the importance of manganese (Mn) is related to its function in chlorophyll synthesis and enzyme activation, such as, the enzymes related to the formation of lignin in the cell wall (Malavolta, 2006), a compound that influence the impermeability and resistance of the seed coat (Capeleti et al, 2005). The limited number of studies have still controversial results as to the action of Mn on the quality of soybean seeds.…”

Section: Introductionmentioning

confidence: 99%

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Qualidade fisiológica de sementes de soja convencional e transgênica RR produzidas sob aplicação foliar de manganês

2014

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Soybean seeds with high physiological quality are essential to achieve high yields, one of the factors affecting seed production is mineral nutrition. The aim of this study was to evaluate the effect of foliar application of manganese, with different doses and stages of application, on the physiological quality of soybean seeds in conventional cultivars and GR descendants with different lignin content in the seed coat. The experiment was conducted in randomized blocks with three replications and a 4 x 4 x 2 factorial arrangement consisting of four soybean cultivars, two conventional and their GR descendants (BRS Celeste and BRS Baliza RR; BRSGO Jataí and BRS Silvânia RR), four doses of Mn via foliar application (0; 200; 400 and 600 g Mn ha -1 ) and two stages of application (R 1 and R 3 ). Before and after six months of storage, the physiological quality of seeds was estimated by testing: germination, accelerated aging, seedling emergence, electrical conductivity and tetrazolium (Viability, vigor and mechanical damage). Lignin contents were determined in the seed coats. The foliar application of Mn provides increased physiological quality in soybean seeds produced. Seeds of the soybean cultivars Celeste and Baliza RR have better physiological quality when compared with Jataí and Silvânia RR. Seeds of soybean cultivars with higher lignin content in the seed coat do not necessarily have better physiological quality, thus seed quality is related to other factors intrinsic to the genotype.Key words: Glycine max, micronutrients, Roundup Ready soybean, vigor.Qualidade fisiológica de sementes de soja convencional e transgênica RR produzidas sob aplicação foliar de manganês Resumo Sementes de soja com alta qualidade fisiológica são essenciais à obtenção de produtividade elevada e um dos fatores que afetam a produção de sementes é a nutrição mineral. O objetivo neste trabalho foi avaliar o efeito da aplicação foliar de manganês, em diferentes doses e estádios de aplicação, na qualidade fisiológica de sementes de soja, em cultivares convencionais e em suas derivadas transgênicas RR, com distintos teores de lignina do tegumento. O ensaio foi conduzido em blocos casualizados, com três repetições e esquema fatorial 4 x 4 x 2, sendo quatro cultivares de soja, duas convencionais e suas derivadas transgênicas RR (BRS Celeste e BRS Baliza RR; BRSGO Jataí e BRS Silvânia RR), quatro doses de Mn via foliar (0; 200; 400 e 600 g Mn ha -1 ) e dois estádios de aplicação (R 1 e R 3 ). A qualidade fisiológica das sementes foi estimada, antes e após seis meses de armazenamento, por meio dos testes: Germinação, Envelhecimento acelerado, Emergência de plântulas, Condutividade elétrica e Tetrazólio (Viabilidade, vigor e danos mecânicos). Realizou-se também a quantificação de lignina no tegumento das sementes. A aplicação foliar de Mn proporciona incrementos na qualidade fisiológica nas sementes de soja produzidas. Sementes das cultivares de soja Celeste e Baliza RR apresentam melhor qualidade fisiológica quando comparadas às de Jataí...

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Seed Coat Development and Dormancy

Debeaujon¹,

Lepiniec²,

Pourcel³

et al.

Seed Development, Dormancy and Germination

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seeds (Olsen, 2004). The cells of the aleurone layer remain alive at seed maturity. In mature Arabidopsis seeds, a thin hyaline layer without pectin also surrounds the embryo (Debeaujon et al., 2000). The testa includes the integument(s) and the chalazal tissues (Fig. 3.1A). The testa consists of several layers of specialized cell types originating from the differentiation of ovular integuments that is triggered by fertilization. They develop from the epidermis of the ovule primordium that derives ontogenetically from the meristematic L1 cell layer (Schneitz et al., 1995). The number of ovule integuments varies depending on plant species. Most monocotyledons (e.g., wheat) and dicotyledons (e.g., Arabidopsis, bean) have two integuments (bitegmic ovules). A single integument (unitegmic ovules) is mainly found in the Rosidae, Ericales, Asteridae and Solanaceae that includes tomato, petunia and tobacco (Boesewinkel and Bouman, 1995; Angenent and Colombo, 1996). The micropyle is a pore formed by the integument(s) as an entrance for the pollen tube. The chalazal region is also an important part of the testa where the connection of the vascular tissues of the maternal funiculus to the seed ends. The scar where the funiculus was connected that remains after seed detachment is called the hilum. The differentiation of testa tissues involves important cellular changes generally ending with programed cell death (PCD). The Arabidopsis testa The Arabidopsis testa involves two integuments, the inner integument (ii) having three cell layers and the outer integument (oi) being two-layered(Schneitz et al., 1995; Beeckman et al., 2000) (Fig. 3.1). In the first few days after fertilization (daf), integument growth proceeds through both cell division and expansion (Haughn and Chaudhury, 2005). At the heart stage of embryo development, the cellular organization of testa tissues becomes evident and distinguishable (Fig. 3.1A), although further modifications are necessary to lead to the formation of the mature testa structure (Fig. 3.1B).

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A new procedure for quantification of lignin in soybean (Glycine max (L.) Merrill) seed coat and their relationship with the resistance to mechanical damage

Cited by 39 publications

References 3 publications

Seleção De Linhagens De Feijão Com Base No Padrão E Na Qualidade De Sementes

Seleção De Linhagens De Feijão Com Base No Padrão E Na Qualidade De Sementes

Qualidade fisiológica de sementes de soja convencional e transgênica RR produzidas sob aplicação foliar de manganês

Seed Coat Development and Dormancy

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