A New Race of<i>Pseudomonas glycinea</i>

Thomas, M. D.; Leary, J. V.

doi:10.1094/phyto-70-310

Cited by 20 publications

(12 citation statements)

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“…Lack of similar reduction in the virulence of other types of deficient mutants, i.e. drug resistance and carbohydrate utilization (Thomas & Leary, 1980), further supports this hypothesis. However, it is contradictory that 38 out of 39 His mutants (Tables 1 and 2) were as virulent as the wild-type.…”

Section: Discussionmentioning

confidence: 53%

“…As previously reported (Thomas & Leary, 1980), we have constructed multiply-marked mutants which are fully as virulent on soybean as the wild-type, by selecting auxotrophic mutants which retain virulence and screening these mutants for virulence before further mutagenesis. These mutants, which have two or three auxotrophic requirements and one or more drug resistance or carbohydrate utilization markers, can provide important information on the spread of disease under field conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Wild-type Pseudomonas glycinea strain PgB3 (race 8), and the complete and minimal media used, have been described earlier (Thomas & Leary, 1980). Each nutritional supplement was added, from filter-sterilized stock solutions to the minimal medium at a final concentration of 20 pg ml-l.…”

Section: Strains and Mediamentioning

confidence: 99%

“…Soybean cultivars Harosoy and Flambeau, which give a susceptible response to PgB3, were inoculated with cells grown from a single colony as reported previously (Thomas & Leary, 1980). Disease symptoms were indexed with respect to severity from 0 to 4 by comparison with symptoms occurring on plants treated with either water (rated 0 or, occasionally, 1) or wild-type PgB3 cells (rated 3 or 4).…”

Section: Strains and Mediamentioning

confidence: 99%

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Mutagenicity and Pathogenicity in Pseudomonas syringae pv. glycinea (Pseudomonas glycinea)

Thomas

Leary

1980

Microbiology

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The isolation and pathogenic response of various drug-resistant and auxotrophic mutants of strain PgB3 of Pseudomonas glycinea, the causal agent of bacterial blight of soybean, are reported. A mutagenesis procedure is described which allows efficient recovery of spontaneous, ethyl methanesulphonate-, hycanthone-, 2-aminopurine-and N-methyl-"-nitro-N-nitrosoguanidine-induced mutants. While many of the auxotrophic mutants were avirulent, fully virulent histidine, adenine and methionine auxotrophic mutants were isolated. The virulence of certain methionine-requiring mutants did not correlate with the amount of methionine required by the mutant. In every instance where revertants to prototrophy were isolated, the revertant regained wild-type virulence.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Strains and Mediamentioning

confidence: 99%

Section: Strains and Mediamentioning

confidence: 99%

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Mutagenicity and Pathogenicity in Pseudomonas syringae pv. glycinea (Pseudomonas glycinea)

Thomas

Leary

1980

Microbiology

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“…At least 8 races of the pathogen have been reported, but more than 210 PLANT BREEDING REVIEWS this number probably exist (Cross et al 1966;Thomas and Leary 1980). Tisselli et al (1980) list cultivars that are resistant to specific races of the pathogen.…”

Section: A Bacterial Blightmentioning

confidence: 99%