A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram.

Uchida, Katsuo; Kudo, Takuji; Suzuki, Ken‐ichiro; Nakase, Takashi

doi:10.2323/jgam.45.49

Cited by 203 publications

(138 citation statements)

References 14 publications

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“…Fatty acid methyl esters and 1,1-dimethoxy-alkanes were analysed by GC and GC\MS as described by Schumann et al (1997) using lyophilized biomass obtained after incubation of strains for 48 h at 28 mC. Determination of the glycolate content was performed according to the colorimetric method of Uchida et al (1999).…”

Section: Methodsmentioning

confidence: 99%

Diversity of grass-associated Microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of Subtercola pratensis sp. nov., Curtobacterium herbarum sp. nov. and Plantibacter flavus gen. nov., sp. nov.

Behrendt¹,

Ulrich²,

Schümann³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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Section: Methodsmentioning

confidence: 99%

Diversity of grass-associated Microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of Subtercola pratensis sp. nov., Curtobacterium herbarum sp. nov. and Plantibacter flavus gen. nov., sp. nov.

Behrendt¹,

Ulrich²,

Schümann³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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“…The amino acids and peptides in peptidoglycan hydrolysates were analysed as described by Groth et al (1999a). The glycolate content of the peptidoglycan was examined by the colorimetric method of Uchida et al (1999). Menaquinones were extracted as described by Collins et al (1977) and were analysed by HPLC (Groth et al, 1997).…”

Section: Notementioning

confidence: 99%

Brachybacterium fresconis sp. nov. and Brachybacterium sacelli sp. nov., isolated from deteriorated parts of a medieval wall painting of the chapel of Castle Herberstein (Austria).

Heyrman¹,

Balcaen²,

Vos³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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NOTEIt is now well recognized that wall paintings can be severely damaged by microbial growth (Ciferri, 1999). In studies on the bacterial community associated with such deterioration, members of the actinomycetes are frequently cultivated (Sorlini et al., 1987 ;Weirich, 1989 ;Karpovich-Tate & Rebrikova, 1990 ; Altenburger et al., 1996 ;Wieser et al., 1999 ;Groth et al., 1999b). During the course of classification of heterotrophic bacteria isolated from deteriorated parts of the wall paintings in the St Catherine chapel of the Castle of Herberstein (Austria), a group of 13 strains was allocated to the genus Brachybacterium. Four of the wall-painting strains (LMG 20333 and 20335-20337) originated from a paint layer sample of black discoloration located in the chancel vault of the chapel ; nine strains (LMG 20338-20346) originated from black stripes in the upper area of the nave's north wall. The strains were isolated from trypticase soy agar (TSA ; BBL) and R2A agar (Difco) with or without added salt ; the isolation procedure used was described previously (Heyrman et al., 1999 Total genomic DNA was purified for 16S rDNA sequencing and REP-PCR using a slight modification of the method of Pitcher et al. (1989), as described by Heyndrickx et al. (1996). For determination of the GjC content and DNA-DNA hybridization, approximately 1 g biomass was harvested from agar plates and prepared further using a combination of the protocols of Marmur (1961) and Pitcher et al. (1989), as described by Logan et al. (2000). REP-PCR genomic fingerprinting was performed with the REP1 and REP2 primers (Versalovic et al., 1994) using the PCR conditions as described by Rademaker & de Bruijn (1997). For each strain, 6 µl PCR product mixed with 2 µl loading buffer (Rademaker & de Bruijn, 1997) was electrophoresed in a 1n5% (w\v) agarose gel and TAE buffer [1n21 g Tris 2-amino-2(hydroxymethyl)-1,3 propandiol l −" , 0n2 ml 0n5 M EDTA l −" , pH 8] for 15 h at a constant 55 V and 4 mC. The first and every sixth lane were loaded with 6 µl of the molecular ruler [45n5% (v\v) 100 bp ruler, 36n5% (v\v) 500 bp ruler and 18 % (v\v) loading buffer]. After staining with ethidium bromide (0n5 µg ml −" ) and visualization, the patterns were digitalized and a Pearson correlation of the resulting band patterns was performed using BioNumerics 2.0 Software (Applied Maths). 16S rDNA sequence analysis was performed as described previously by Heyrman & Swings (2001). A phylogenetic tree was constructed based on global alignment and the neighbour-joining method, including all described members of the genus Brachybacterium, and Dermabacter hominis as an outgroup. A similarity matrix was constructed using pairwise alignment of the sequences and the unweighted pair group method with arithmetic averages (UPGMA) algorithm. The GjC content of DNA was determined by HPLC as described by Mesbah et al. (1989) using a Waters Symmetry Shield RP ) column thermostatically controlled at 37 mC. The solvent was 0n02 M NH % H # PO % with 1n5 % acetonitrile (pH 4n0). Non-met...

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“…The strains were grown in BHI broth for 5 days at 30 uC and the resultant biomass was washed twice in distilled water and freeze-dried. Established methods were used for the extraction and analysis of fatty acids (Komagata & Suzuki, 1987), muramic acid type (Uchida et al, 1999), polar lipids (Minnikin et al, 1984) and wholeorganism sugars (Schaal, 1985). Qualitative analysis of the amino acids was performed using a standard procedure (Schleifer & Kandler, 1972;Schleifer, 1985).…”

mentioning

confidence: 99%

Agrococcus casei sp. nov., isolated from the surfaces of smear-ripened cheeses

Bora

Vancanneyt

Gelsomino

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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Seven Gram-positive, coryneform bacteria with virtually identical whole-organism protein patterns were isolated from the surface of smear-ripened cheeses. Representatives of these strains were the subject of a polyphasic study designed to establish their taxonomic status. The organisms formed a distinct branch in the Microbacteriaceae 16S rRNA gene tree and were most closely related to members of the genus Agrococcus, sharing sequence similarities of 95.4-98.7 %. The chemotaxonomic profiles of the strains were consistent with their classification in the genus Agrococcus. The combined genotypic and phenotypic data show that the isolates should be classified in the genus Agrococcus as representatives of a novel species. The name Agrococcus casei sp. nov. is proposed for this taxon. Isolate R-17892t2 T (=DSM 18061 T =LMG 22410 T ) is the type strain of Agrococcus casei sp. nov.The complex consortium of micro-organisms found on the surfaces of smear-ripened cheeses includes major populations of coryneform bacteria (catalase-positive irregular rods and cocci), staphylococci (catalase-positive cocci) and yeasts (Valdés-Stauber et al., 1997;Carnio et al., 1999;Bockelmann & Hoppe-Seyler, 2001). Until recently, cheese coryneform bacteria were assigned to groups based on a few subjectively weighted morphological and staining properties (Piton-Malleret & Gorrieri, 1992;Eliskases-Lechner & Ginzinger, 1995). Such studies have been replaced by polyphasic taxonomic investigations, which show that the coryneform component of smear-ripened cheeses contains members of novel taxa, as exemplified by the isolation and description of Corynebacterium casei and Microbacterium gubbeenense from the surface of Gubbeen cheese (Brennan et al., 2001a(Brennan et al., , b, 2002 and Arthrobacter arilaitensis and Arthrobacter bergerei from diverse French smear-ripened cheeses (Irlinger et al., 2005). The present study was designed to determine the taxonomic status of a homogeneous group of coryneform bacteria isolated from smear-ripened cheeses and presumptively assigned to the genus Agrococcus.The genus Agrococcus was proposed by Groth et al. (1996) for two Gram-positive, coryneform bacteria that could be distinguished from members of other genera classified in the family Microbacteriaceae using genotypic and phenotypic criteria. The genus currently contains four species with validly described names: Agrococcus baldri Zlamala et al. The phylogenetic positions of the four representative cheese isolates were determined by 16S rRNA gene sequence analysis. Biomass from growth in brain-heart infusion broth (BHI; Difco) for 5 days at 30 uC was checked for purity, harvested by centrifugation, washed in NaCl/EDTA buffer (0.1 M EDTA, 0.1 M NaCl, pH 8.0) and stored at 220 u C until required. Genomic DNA was extracted as described by Sambrook & Russell (2001) and used as a template for PCR amplification and sequencing following the procedure of Kim et al. (1998). The resultant almost complete 16S rRNA gene sequences (1466-1470 nt) were manually aligned ...

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A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram.

Cited by 203 publications

References 14 publications

Diversity of grass-associated Microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of Subtercola pratensis sp. nov., Curtobacterium herbarum sp. nov. and Plantibacter flavus gen. nov., sp. nov.

Diversity of grass-associated Microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of Subtercola pratensis sp. nov., Curtobacterium herbarum sp. nov. and Plantibacter flavus gen. nov., sp. nov.

Brachybacterium fresconis sp. nov. and Brachybacterium sacelli sp. nov., isolated from deteriorated parts of a medieval wall painting of the chapel of Castle Herberstein (Austria).

Agrococcus casei sp. nov., isolated from the surfaces of smear-ripened cheeses

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