A New Role for Follicle-Stimulating Hormone in the Regulation of Calcium Flux in Sertoli Cells: Inhibition of Na+/Ca++ Exchange*

Grasso, Patricia; Joseph, Michelle P.; Reichert, Leo E.

doi:10.1210/endo-128-1-158

Cited by 26 publications

(5 citation statements)

References 26 publications

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“…The calcium-phospholipid-dependent pathway in cultured immature rat Sertoli cells is not activated by FSH [25,26], and effects of FSH on cytosolic calcium levels are dependent on an influx of calcium. The mechanism of this influx is presently controversial: a) FSH-induced uptake of calcium is directly [6] or indirectly [27] coupled to a hypothetical FSH-operated calcium channel and b) it has been demonstrated that FSH hormone and FSH receptors modulate Na+/Ca 2 + exchange [28], c) bind calcium [29], or d) activate calcium channels [6,7]. Results with 4 5 Ca 2 + and fura-2 strongly suggest voltage-sensitive and voltage-independent calcium channels in freshly isolated [7] or cultured Sertoli cells [30].…”

Section: Discussionmentioning

confidence: 99%

Voltage-Dependent Calcium Current with Properties of T-Type Current in Sertoli Cells from Immature Rat Testis in Primary Cultures1

Lalevée¹,

Pluciennik²,

Joffre³

1997

Biology of Reproduction

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We have applied the whole-cell configuration of the patch-clamp technique to investigate calcium currents in Sertoli cells from immature rat testis in primary culture. Cesium-filled pipettes were used to block potassium currents. In the presence of 10 mM extracellular calcium (Ca+), depolarizations elicited transient inward currents in the range -70 to +10 mV with a maximal amplitude of -1.3 pA/pF at -30 mV. This component activated in the range -70 to -20 mV (V0.5 = -42 mV) and inactivated in the range -75 to -45 mV (V0.5 = 61 mV). Currents were not modified when barium (Ba2+) was substituted for Ca2+. They were suppressed by Ca(2+)-free solutions, insensitive to Bay K 8644 (an L-type channel opener), and inhibited by 1 mM cobalt, 10 microM nickel, 10 microM isradipine, and 1-10 microM omega conotoxine GVIA (four calcium-channel blockers). We conclude that calcium channels with the properties of T-type calcium channels of excitable cells are located in the membrane of immature Sertoli cells in primary cultures. Because these channels do not appear to be directly sensitive to FSH, their involvement in calcium movements remains to be determined.

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Section: Discussionmentioning

confidence: 99%

Voltage-Dependent Calcium Current with Properties of T-Type Current in Sertoli Cells from Immature Rat Testis in Primary Cultures1

Lalevée¹,

Pluciennik²,

Joffre³

1997

Biology of Reproduction

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“…Na + accumulation can also activate the Na + /Ca 2+ exchanger in a reverse mode where Ca 2+ is imported and Na + removed from the cell and both transporters contribute to cytosolic calcium accumulation. 43 All these conditions can give rise to a significant alteration of cellular behavior as exocytosis and/or nuclear transcription, which in turn may be a way to regulate cell metabolism in actively secreting cells. It has been shown previously that cAMP/PKA acts as a mediator of both 1,25D 3 genomic and nongenomic responses in a variety of cell systems.…”

Section: ■ Discussionmentioning

confidence: 99%

“…It has been postulated that Na + /K + ATPase inhibition, and consequently Na + accumulation, evokes membrane depolarization and VDCC opening. Na + accumulation can also activate the Na + /Ca 2+ exchanger in a reverse mode where Ca 2+ is imported and Na + removed from the cell and both transporters contribute to cytosolic calcium accumulation . All these conditions can give rise to a significant alteration of cellular behavior as exocytosis and/or nuclear transcription, which in turn may be a way to regulate cell metabolism in actively secreting cells.…”

Section: Discussionmentioning

confidence: 99%

1α,25-Dihydroxyvitamin D₃ Signaling Pathways on Calcium Uptake in 30-Day-Old Rat Sertoli Cells

et al. 2011

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1α,25-Dihydroxyvitamin D(3) (1,25D(3)) is the active metabolite of vitamin D(3) and the major calcium regulatory hormone in tissues. The aim of this work was to investigate the mechanism of action of 1,25D(3) on (45)Ca(2+) uptake in Sertoli cells from 30-day-old rats. Results showed that 10(-9) and 10(-12) M 1,25D(3) increased the rate of (45)Ca(2+) uptake 5 and 15 min after hormone exposure and that 1α,25(OH)(2) lumisterol(3) (JN) produced a similar effect suggesting that 1,25D(3) action occurs via a putative membrane receptor. The involvement of voltage-dependent calcium channels (VDCC) in 1,25D(3) action was evidenced by using nifedipine, while the use of Bapta-AM demonstrated that intracellular calcium was not implicated. Moreover, the incubation with ouabain and digoxin increased the rate of (45)Ca(2+) uptake, indicating that the effect of 1,25D(3) may also result from Na(+)/K(+)-ATPase inhibition. In addition, we demonstrated that the mechanism underlying the hormone action involved extracellular signal-regulated kinase (ERK) and protein kinase C (PKC) activation in a phospholipase C-independent way. Furthermore, a local elevation of the level of cAMP, as demonstrated by incubating cells with dibutyryl cAMP or a phosphodiesterase inhibitor, produced an effect similar to that of 1,25D(3), and the inhibition of protein kinase A (PKA) nullified the hormone action. In conclusion, the stimulatory effect of 1,25D(3) on (45)Ca(2+) uptake in Sertoli cells occurs via VDCC, as well as PKA, PKC, and ERK activation. These protein kinases seem to act by inhibiting Na(+)/K(+)-ATPase or directly phosphorylating calcium channels. The Na(+)/K(+)-ATPase inhibition may result in Na(+)/Ca(2+) exchanger activation in reverse mode and consequently induce the uptake of calcium into the cells.

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“…The hormonal sugar moiety makes specific interactions with the surface of the receptor cavity. While strongly-bonded low-density water was a barrier to movement of Ca" through the channel which has been proposed in the hFSH receptor, [26,27], weaklybonded water induced by accumulation of anionic groups, K+ and water, attracts Ca?' into the cavity, from which it diffuses through its transmembrane channel to elicit the biological response.…”

Section: Feds Lettersmentioning

confidence: 98%

An antipodean perception of the mode of action of glycoprotein hormones

Renwick

Wiggins

1992

FEBS Letters

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Continuan~ of vertebrate species and maintenance of mgtabolim~ have an absolute requirement for the glycoprotein hormones of the anterior pituitary gland. It is now firmly accepted that the N-glycans of these and the related placental hormone, ehorionic gonadotropin, have ~ntial if undefined roles in their mechanism of action. However, recent investigations by Weisshaar and his eolleagu~ on the oligosaeeharides of haman luteinizing hormone and chorionie gonadotropin, do not support the generally accepted view of carbohydrates in hormone-rc~ptor interactions and a further concept is propo~xl that invokes negative charges and changes in structured water.Glycoprotein hormone; N-Glycan; Structured waterThe placental glycoprotein hormone, chorionic gonadotropin (CG), is closely related in structure to three hormones that are synthesized and secreted by the anterior pituitary gland in all vertebrates, viz. follicle-stimulating hormone (FSH, follitropin), luteinizing hormone (LH, lutropin) and thyroid stimulating hormone (TSH, thyrotropin). FSH and LH are intrinsic components of the reproductive process, CG like LH stimulates ovary and testis and TSH is an essential regulator of thyroid structure and function.Each hormone consists of two dissimilar subunits and fl, which are highly cross-linked by disulphide bonds, five in each ~ and six in each fl subunit. While the primary structures of the former and their sites of carbohydrate alttachment are virtually the same in all four hormones, the amino acid sequences of the fl elements are unique, in part homologous, and their oligosaceharides differ. The oligosaeeharides in the pituitary glycoprotein hormones are attached through a proximal N-acetylglucosamine residue to the amide nitrogen of asparagine.Chorionic gonadotropin differs in having a C-terminal extension of 30-35 residues in the ,6 subunit that contains four additional oligosaceharide chains Olinked to serine residues in the human hormone. Each subunit is biologically inert, but when associated noncovalently they form the hormonally active heterodimer the specificity of which is conferred by theft component [1].Correspondence address: A. Reawicl~, Department el' Bioehemi~try, University of Auckland, Private Bag 92019, Auckland, New Zealand. Fax: (64) (9) 302 1795.The functions of the N-glycans in the biological actions of these hormones have been inferred from numerous investigations that mainly employed hCG and it is now widely accepted that while the sugar chains are not required for hormone-receptor binding, their presence is essential for activation of the adenylate cyclase system [2-6]. More recent reports provide evidence for subunitand site-specific functions of these oligosaeeharides; those on the ~ subunito primarily at Ash-52, are seemingly more important with respect to hormone assembly, secretion and signal transduction [7][8][9].While crystals of hCG suitable for X-ray diffraction have been obtained [10], no data are yet available. However, one model [3] suggests that the N-glycans of hCG may intera...

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A New Role for Follicle-Stimulating Hormone in the Regulation of Calcium Flux in Sertoli Cells: Inhibition of Na+/Ca++ Exchange*

Cited by 26 publications

References 26 publications

Voltage-Dependent Calcium Current with Properties of T-Type Current in Sertoli Cells from Immature Rat Testis in Primary Cultures1

Voltage-Dependent Calcium Current with Properties of T-Type Current in Sertoli Cells from Immature Rat Testis in Primary Cultures1

1α,25-Dihydroxyvitamin D₃ Signaling Pathways on Calcium Uptake in 30-Day-Old Rat Sertoli Cells

An antipodean perception of the mode of action of glycoprotein hormones

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A New Role for Follicle-Stimulating Hormone in the Regulation of Calcium Flux in Sertoli Cells: Inhibition of Na+/Ca++ Exchange*

Cited by 26 publications

References 26 publications

Voltage-Dependent Calcium Current with Properties of T-Type Current in Sertoli Cells from Immature Rat Testis in Primary Cultures1

Voltage-Dependent Calcium Current with Properties of T-Type Current in Sertoli Cells from Immature Rat Testis in Primary Cultures1

1α,25-Dihydroxyvitamin D3 Signaling Pathways on Calcium Uptake in 30-Day-Old Rat Sertoli Cells

An antipodean perception of the mode of action of glycoprotein hormones

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1α,25-Dihydroxyvitamin D₃ Signaling Pathways on Calcium Uptake in 30-Day-Old Rat Sertoli Cells