2008
DOI: 10.1016/s1472-6483(10)60219-2
|View full text |Cite
|
Sign up to set email alerts
|

A new safe, simple and successful vitrification method for bovine and human blastocysts

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

6
44
0
5

Year Published

2009
2009
2024
2024

Publication Types

Select...
4
2

Relationship

0
6

Authors

Journals

citations
Cited by 67 publications
(55 citation statements)
references
References 39 publications
6
44
0
5
Order By: Relevance
“…Furthermore, the distinct developmental stages of the embryos in different groups did not affect overall cell survival. Overall, these results confirmed that when combined with ethylene glycol, both DMSO, with its fast penetrating characteristics and low molecular weight, and glycerol, with higher molecular weight but moving across the plasma membrane predominantly by facilitated diffusion through aquaporins 3 [26], are efficient cryoprotectants for blastocyst vitrification, as earlier demonstrated by Stachecki et al [12,13]. Thus, blastocyst vitrification could be effectively achieved with larger carriers, which were sealed to avoid cross contamination during long term storage, using longer loading and exposure times and without the need of blastocoel collapse.…”
Section: Discussionsupporting
confidence: 86%
See 4 more Smart Citations
“…Furthermore, the distinct developmental stages of the embryos in different groups did not affect overall cell survival. Overall, these results confirmed that when combined with ethylene glycol, both DMSO, with its fast penetrating characteristics and low molecular weight, and glycerol, with higher molecular weight but moving across the plasma membrane predominantly by facilitated diffusion through aquaporins 3 [26], are efficient cryoprotectants for blastocyst vitrification, as earlier demonstrated by Stachecki et al [12,13]. Thus, blastocyst vitrification could be effectively achieved with larger carriers, which were sealed to avoid cross contamination during long term storage, using longer loading and exposure times and without the need of blastocoel collapse.…”
Section: Discussionsupporting
confidence: 86%
“…The results obtained with the Fast Freeze direct plunge were in accordance with those observed in a preliminary study where a small sample of surplus human blastocysts donated for research were vitrified with the S3 method (basis of the Global Fast Freeze media, using a protocol similar to that used in the Fast Freeze-direct plunge group) and subsequently stained, leading to a survival rate following warming of 84 % and a cell survival rate of 87 % [12]. Thus, we could document and confirm that the simplified version of the Fast Freeze protocol, including the direct plunge of the freezing straw into liquid nitrogen (Fast Freezedirect plunge), leads to equivalent results to those obtained with the original protocol version (S3 vitrification system; [13]).…”
Section: Discussionsupporting
confidence: 86%
See 3 more Smart Citations