A New Self-Assembling System for Targeted Gene Delivery

Eaton, Michael A. W.; Baker, Terence S.; Catterall, Catherine; Crook, Kenneth E.; Macaulay, G. S.; Mason, Barbara; Norman, Timothy J.; Parker, David; Perry, Justin J.; Taylor, Richard J. K.; Turner, Alison; Weir, Ashley

doi:10.1002/1521-3757(20001117)112:22<4229::aid-ange4229>3.0.co;2-1

Cited by 13 publications

(7 citation statements)

References 22 publications

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“…Polymer gene delivery is considered a promising alternative to viral vectors1, 2 owing to its safety and the ready availability of a variety of polymer carriers such as polyethyleneimine (PEI)3–5 and poly(β‐aminoester)s,6 whose structures may be optimized by using high‐throughput methods 7. Nanoparticles with surface‐coated ligands8 or magnetic properties,9 layered structures,10 linear‐dendritic polymers,11 triblock copolymers,12 polyion micelles,13 surface‐charge‐shielded PEI polyplexes,14 dendrimers,15 and inorganic nanotubes16 have also been tested as carriers.…”

Section: Methodsmentioning

confidence: 99%

Virion‐Mimicking Nanocapsules from pH‐Controlled Hierarchical Self‐Assembly for Gene Delivery

et al. 2008

Angew Chem Int Ed

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A new concept for fabricating nanocarriers carrying free DNA for gene delivery to overcome the intracellular dissociation barrier of cationic polymer/DNA complexes is presented. Free DNA plasmids (shown in green) are encapsulated in a nanocapsule core, which is protected by a hydrophobic membrane (purple) with a poly(ethylene glycol) outer layer (blue). The nanocapsules can release free DNA into the cells and have high in vitro and in vivo transfection efficiency.

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Section: Methodsmentioning

confidence: 99%

Virion‐Mimicking Nanocapsules from pH‐Controlled Hierarchical Self‐Assembly for Gene Delivery

et al. 2008

Angew Chem Int Ed

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“…However, classic architectures can still be optimized in surprising ways 10. Nevertheless, the above observations have induced a search for new modular self‐assembling platforms that try to combine features of the first‐generation vectors 11…”

Section: Dye‐exclusion Assay13 [A]mentioning

confidence: 99%

Amphiphilic Dendrimers: Novel Self‐Assembling Vectors for Efficient Gene Delivery

et al. 2003

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Ein scharfes Maximum in der Struktur‐Aktivitäts‐Beziehung, ein überraschend starker Einfluss der hydrophoben Teilstruktur auf DNA‐Bindung und ‐Transport und ein ungewöhnlicher Serum‐Effekt auf die relativen Transfektionsaktivitäten wurden in ersten biologischen Studien an einer Serie von vier amphiphilen Dendrimeren gefunden, die als Transfektionsagentien entwickelt wurden. Das Dendrimer der niedrigsten Generation, 1, erwies sich als das aktivste.

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“…It has been reported that bolasomes prepared from cationic bolaforms in the presence of lipids such as 1,2-dioleyl-sn-glycero-3-phosphoethanolamine are capable of delivering genes to target cells. 29 Following the report by Eaton and co-workers 30 on using cationic bolaforms as a nonviral gene delivery vector, several cell-and mitochondria-targeting cationic bolaforms have been developed. 31−35 Although the transfection efficiency of these bolaforms was observed to be not satisfactory, the cationic surfaces have been shown to promote transcytosis enhancing vesicle transport across biological barriers, 36 mainly through the blood−brain barrier (BBB).…”

Section: ■ Introductionmentioning

confidence: 99%

Self-Assembly, In Vitro Gene Transfection, and Antimicrobial Activity of Biodegradable Cationic Bolaamphiphiles

et al. 2023

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Bolaamphiphiles or bolaforms have drawn particular interest in drug and gene delivery, and studies of bolaforms have been growing continuously. Bolaforms, due to their unique structure, exhibit specific self-assembly behavior in water. The present work aims to develop biodegradable cationic bolaforms with a better gene transfection ability. In this work, a novel cationic bolaform (Bola-1) with head groups bearing hydroxyl (OH) functionality was designed and synthesized to investigate self-assembly and gene transfection efficiency. The selfassembly behavior of Bola-1 in water was compared with that of the hydrochloride salt (Bola-2) of its precursor molecule to investigate the effect of the −OH functionality on their solution properties. Several techniques, including surface tension, electrical conductivity, fluorescence probe, calorimetry, dynamic light scattering, and atomic force microscopy, were employed for the physicochemical characterization of Bola-1 and Bola-2. Despite the presence of polar urea groups in the spacer chain, both bolaforms were found to form spherical or elongated micelles above a relatively low critical aggregation concentration (CAC). The presence of the OH group was found to significantly affect the CAC value. The results of calorimetric measurements suggested a thermodynamically favorable aggregate formation in salt-free water. Despite stronger binding efficiency with calf thymus DNA, in vitro gene transfection studies performed using adherent cell Hek 293 suggested that both Bola-1 and Bola-2 have gene transfection efficiency comparable to that of turbofectamine standard. Both bolaforms were found to exhibit significant in vitro cytotoxicity at higher concentrations. Also, the bolaforms showed beneficial antibacterial activity at higher concentrations.

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A New Self-Assembling System for Targeted Gene Delivery

Cited by 13 publications

References 22 publications

Virion‐Mimicking Nanocapsules from pH‐Controlled Hierarchical Self‐Assembly for Gene Delivery

Virion‐Mimicking Nanocapsules from pH‐Controlled Hierarchical Self‐Assembly for Gene Delivery

Amphiphilic Dendrimers: Novel Self‐Assembling Vectors for Efficient Gene Delivery

Self-Assembly, In Vitro Gene Transfection, and Antimicrobial Activity of Biodegradable Cationic Bolaamphiphiles

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