A new sensitive test based on yeast cells for studying environmental pollution

Terziyska, Anna; Waltschewa, Liliana W.; Venkov, Pencho

doi:10.1016/s0269-7491(99)00237-7

Cited by 32 publications

(8 citation statements)

References 19 publications

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“…Cells carrying multiple olfactory receptors are the most common configuration employed as a recognition element. Bacterial and yeast cells are some common examples of cells used [ 174 , 175 ]. Implementation of isolated OR-proteins or peptides instead of whole cells has been intensively researched due to their potential to scale down bioelectronic devices and achieve better sensitivity and selectivity rates [ 176 ].…”

Section: Gas Sensors For Vocs Detectionmentioning

confidence: 99%

Advancements in Microfabricated Gas Sensors and Microanalytical Tools for the Sensitive and Selective Detection of Odors

Ollé

Farré-Lladós

Casals‐Terré

2020

Sensors

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In recent years, advancements in micromachining techniques and nanomaterials have enabled the fabrication of highly sensitive devices for the detection of odorous species. Recent efforts done in the miniaturization of gas sensors have contributed to obtain increasingly compact and portable devices. Besides, the implementation of new nanomaterials in the active layer of these devices is helping to optimize their performance and increase their sensitivity close to humans’ olfactory system. Nonetheless, a common concern of general-purpose gas sensors is their lack of selectivity towards multiple analytes. In recent years, advancements in microfabrication techniques and microfluidics have contributed to create new microanalytical tools, which represent a very good alternative to conventional analytical devices and sensor-array systems for the selective detection of odors. Hence, this paper presents a general overview of the recent advancements in microfabricated gas sensors and microanalytical devices for the sensitive and selective detection of volatile organic compounds (VOCs). The working principle of these devices, design requirements, implementation techniques, and the key parameters to optimize their performance are evaluated in this paper. The authors of this work intend to show the potential of combining both solutions in the creation of highly compact, low-cost, and easy-to-deploy platforms for odor monitoring.

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Section: Gas Sensors For Vocs Detectionmentioning

confidence: 99%

Advancements in Microfabricated Gas Sensors and Microanalytical Tools for the Sensitive and Selective Detection of Odors

Ollé

Farré-Lladós

Casals‐Terré

2020

Sensors

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“…Therefore, it is necessary to increase cell wall permeability before using them as WCBs, which constitutes an additional difficulty. Mutants with enhanced cell permeability can be used for this purpose ( Terziyska et al, 2000 ; Walmsley and Keenan, 2000 ).…”

Section: Yeasts As Heavy Metal Wcbsmentioning

confidence: 99%

Heavy metal whole-cell biosensors using eukaryotic microorganisms: an updated critical review

2015

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This review analyzes the advantages and disadvantages of using eukaryotic microorganisms to design whole-cell biosensors (WCBs) for monitoring environmental heavy metal pollution in soil or aquatic habitats. Basic considerations for designing a eukaryotic WCB are also shown. A comparative analysis of the promoter genes used to design WCBs is carried out, and the sensitivity and reproducibility of the main reporter genes used is also reviewed. Three main eukaryotic taxonomic groups are considered: yeasts, microalgae, and ciliated protozoa. Models that have been widely analyzed as potential WCBs are the Saccharomyces cerevisiae model among yeasts, the Tetrahymena thermophila model for ciliates and Chlamydomonas model for microalgae. The advantages and disadvantages of each microbial group are discussed, and a ranking of sensitivity to the same type of metal pollutant from reported eukaryotic WCBs is also shown. General conclusions and possible future developments of eukaryotic WCBs are reported.

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“…In this study, yeast was selected as solid support substrate in the imprinting process. Compared with other supports such as silica beads , chitosan beads , and polymer particles , yeast has the advantages of low cost, easily available source , and abundant active biomolecule on the cell wall without further modification process. Considering the above advantages, yeast may be chosen as a promising support substrate in the molecularly imprinting process.…”

Section: Introductionmentioning

confidence: 99%

Removal of cefalexin using yeast surface‐imprinted polymer prepared by atom transfer radical polymerization

Pan

Dai

et al. 2012

J of Separation Science

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The first use of yeast as a support in the molecular imprinting field combined with atom transfer radical polymerization was described. Then, the as-prepared molecularly imprinted polymers were characterized by Fourier transmission infrared spectrometry, scanning electron microscope, thermogravimetric analysis, and elemental analysis. The obtained imprinted polymers demonstrated elliptical-shaped particles with the thickness of imprinting layer of 0.63 μm. The batch mode experiments were adopted to investigate the adsorption equilibrium, kinetics, and selectivity. The kinetic properties of imprinted polymers were well described by the pseudo-second-order kinetic equation, indicating the chemical process was the rate-limiting step for the adsorption of cefalexin (CFX). The equilibrium data were well fitted by the Freundlich isotherm, and the multimolecular layers adsorption capacity of imprinted polymers was 34.07 mg g(-1) at 298 K. The selectivity analysis suggested that the imprinted polymers exhibited excellent selective recognition for CFX in the presence of other compounds with related structure. Finally, the analytical method based on the imprinted polymers extraction coupled with high-performance liquid chromatograph was successfully used for CFX analysis in spiked pork and water samples.

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A new sensitive test based on yeast cells for studying environmental pollution

Cited by 32 publications

References 19 publications

Advancements in Microfabricated Gas Sensors and Microanalytical Tools for the Sensitive and Selective Detection of Odors

Advancements in Microfabricated Gas Sensors and Microanalytical Tools for the Sensitive and Selective Detection of Odors

Heavy metal whole-cell biosensors using eukaryotic microorganisms: an updated critical review

Removal of cefalexin using yeast surface‐imprinted polymer prepared by atom transfer radical polymerization

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