A New, Simple Method for Cold Storage of the Pancreas Using Perfluorochemical

Kuroda, Yoshikazu; Kawamura, Takashi; Suzuki, Y; Fujiwara, Hidetoshi; Yamamoto, Kentaro; Saitoh, Yoichi

doi:10.1097/00007890-198809000-00027

Cited by 106 publications

(4 citation statements)

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“…In addition to their oxygen carrying capacity, PFC suspensions are optically white and simply scatter light with no specific absorbance, making them a suitable candidate for optical studies in perfused hearts. While they were originally developed for medical applications as a potential blood substitute for transfusions, organ transplants, and battlefield medicine (26,38,39,47), PFCs were not successfully translated to in vivo human use due to chronic toxicity. Important for this study, no acute impact of these agents on organ functions has been reported, underscoring the potential benefit of using PFCs in terminal perfusion experiments.…”

Section: Introductionmentioning

confidence: 99%

Cardiac performance is limited by oxygen delivery to the mitochondria in the crystalloid-perfused working heart

Kuzmiak-Glancy

Covián

Femnou

et al. 2018

American Journal of Physiology-Heart and Circulatory Physiology

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The left ventricular working, crystalloid-perfused heart is used extensively to evaluate basic cardiac function, pathophysiology, and pharmacology. Crystalloid-perfused hearts may be limited by oxygen delivery, as adding oxygen carriers increases myoglobin oxygenation and improves myocardial function. However, whether decreased myoglobin oxygen saturation impacts oxidative phosphorylation (OxPhos) is unresolved, since myoglobin has a much lower affinity for oxygen than cytochrome c oxidase (COX). In the present study, a laboratory-based synthesis of an affordable perfluorocarbon (PFC) emulsion was developed to increase perfusate oxygen carrying capacity without impeding optical absorbance assessments. In left ventricular working hearts, along with conventional measurements of cardiac function and metabolic rate, myoglobin oxygenation and cytochrome redox state were monitored using a novel transmural illumination approach. Hearts were perfused with Krebs-Henseleit (KH) or KH supplemented with PFC, increasing perfusate oxygen carrying capacity by 3.6-fold. In KH-perfused hearts, myoglobin was deoxygenated, consistent with cytoplasmic hypoxia, and the mitochondrial cytochromes, including COX, exhibited a high reduction state, consistent with OxPhos hypoxia. PFC perfusate increased aortic output from 76 ± 6 to 142 ± 4 ml/min and increased oxygen consumption while also increasing myoglobin oxygenation and oxidizing the mitochondrial cytochromes. These results are consistent with limited delivery of oxygen to OxPhos resulting in an adapted lower cardiac performance with KH. Consistent with this, PFCs increased myocardial oxygenation, and cardiac work was higher over a wider range of perfusate Po. In summary, heart mitochondria are limited by oxygen delivery with KH; supplementation of KH with PFC reverses mitochondrial hypoxia and improves cardiac performance, creating a more physiological tissue oxygen delivery. NEW & NOTEWORTHY Optical absorbance spectroscopy of intrinsic chromophores reveals that the commonly used crystalloid-perfused working heart is oxygen limited for oxidative phosphorylation and associated cardiac work. Oxygen-carrying perfluorocarbons increase myocardial oxygen delivery and improve cardiac function, providing a more physiological mitochondrial redox state and emphasizing cardiac work is modulated by myocardial oxygen delivery.

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Section: Introductionmentioning

confidence: 99%

Cardiac performance is limited by oxygen delivery to the mitochondria in the crystalloid-perfused working heart

Kuzmiak-Glancy

Covián

Femnou

et al. 2018

American Journal of Physiology-Heart and Circulatory Physiology

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“…After the clean-up in back-table, the pancreas is transported to the Islet Isolation Unit using the two-layer method. 37 Islet isolation is performed using the method described by Lineltsky et al a lengthy procedure which lasts about 8-12 h and is highly specialized. 38 The islets are stained with the insulin-specific Dithizone stain and counted by optical microscopy and the results are expressed in IEQ.…”

Section: Discussionmentioning

confidence: 99%

Islet vs. pancreas transplantation in Brazil: Defining criteria for pancreas allocation decision

Sá

Sogayar

Eliaschewitz

et al. 2011

Islets

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“…In spite of promising experiments with perfluorocarbons as media for liquid ventilation these compounds have not yet become used for diving. However, they are widely used in medicine as media for curing premature babies, 13,14 in lung healing processes, 15,16 for transplantation/organ preservation, 17,18 as blood substitutes 19,20 and oxygen carriers 19,21 or for eye surgery. 22 Since fluorinated compounds have such an amazing ability to dissolve a large amount of gases, the question arises if their crystalline form reflects the behavior in the liquid state showing some voids present in the structure.…”

Section: Introductionmentioning

confidence: 99%