No abstract

No abstract

No abstract

U I t rast ruct u ra I a na I yses of pancreatic grafts preserved by the two-layer cold-storage method and by simple cold storage in University of Wisconsin solutionAbstract The two-layer cold storage method (TLM) using University of Wisconsin (UW) solution supplies sufficient oxygen to pancreatic grafts during preservation and extends pancreas preservation time to up to 96h in the canine model. Simple cold storage in UW (UWM) on the other hand, preserves canine pancreas grafts for up to 72h by preventing cell swelling, mainly because of its high osmotic pressure. The aim of this study is to analyze morphologically dog pancreatic grafts preserved by these two methods with their different mechanisms. Immediately after preservation of canine pancreata by TLM for 72h and 96h (group 1 and group 3, respectively), and by UWM for 72h and 96h (group 2 and group 4, respectively), tissue ATP levels were determined using high-performance liquid chromatography (HPLC), and detailed morphological analyses of intragraft components were performed using light-and electron microscopy. The mean areas of one mitochondrion and rough endoplasmic reticulum (RER) vacuolization were calculated by computer-graphic analyses using NIH image 1.62f soft. The tissue ATP levels were significantly higher in groups 1 and 3 than groups 2 and 4 (P < 0.05).Light microscopy demonstrated no marked difference among the 4 groups. By electron microscopy however, mitochondria1 swelling and RER vacuolization were observed in acinar cells to various extents in the 4 groups. They were significantly more evident in group 2 than group 1 (P < 0.05), and in group 4 than group 3 ( P < 0.05).In conclusion, TLM demonstrated excellent protection of intracellular organelles, mitochondria, and RER, up to 72-96h. Well-maintained graft ATP levels in TLM groups may result in maintaining the integrity of intracellular organelle membranes as well as cellular membranes. Keywords

We tested the preservation of the pancreas for 96 h by a modified two‐layer (UW solution/perfluorochemical) cold storage method (group 1) in the canine model of pancreas autotransplantation and compared this with an original two‐layer (Euro‐Collins' solution/perfluorochemical) cold storage method (group 2) and simple cold storage method with UW solution (group 3). A graft was considered functioning if the dog had a normal blood glucose for at least 5 days after transplantation. The functional success rates after preservation for 72 11 were 100%, 100% and 80% for groups 1, 2 and 3 respectively. On the other hand, the functional success rates for groups 1, 2 and 3 after preservation for 96 h were 75%, 0% and 0% respectively. The mean K value of 96‐hour preserved grafts for group 1 at 2 weeks after transplantation was 1.52 ± 0.30 compared with 1.98 ± 0.48 before preservation. Biopsies of grafts from group 1 showed almost normal pancreatic architecture even after preservation for 96 h. In addition, biopsies of grafts preserved for 9611 in group 1 at 4 weeks after transplantation showed almost normal endocrine tissue with mild fibrosis of the exocrine tissue. This study demonstrated the possibility of preserving the pancreas for 96 h prior to transplantation.