A new species of Diderma from Bidoup Nui Ba National Park (southern Vietnam)

Abstract

A new species of Diderma, described herein as D. dalatense, was found in ground leaf litter in mixed montane tropical forests (Bidoup Nui Ba National Park) of southern Vietnam. This species was discovered during intensive studies on the distribution and ecology of fungi and myxomycetes in tropical forests of Vietnam in the context of a long-term project Ecolan 1.2. The morphology of representative specimens was examined by light and scanning electron microscopy, and micrographs of relevant details are provided. D. dalatense has unique combination of morphological characters of the sporocarps among species of Diderma including very small (0.2-0.4 mm in diam.) dark-yellow, globose to subglobose sessile sporocarps, large conical or subglobose yellow columella, smooth peridium with three layers resembling that of Leocarpus and irregularly warted spores. In addition to the morphological description, partial sequences of three genetic markers of this new species (SSU, EF1A, COI) were obtained and submitted to GenBank. The stability of the taxonomic characters of the species was confirmed by several collections obtained in different localities of the National Park.

<i>Lamproderma vietnamense</i>: a new species of myxomycetes with reticulate spores from Phia Oắc - Phia Đén National Park (northern Vietnam) supported by molecular phylogeny and morphological analysis

“…In addition, partial sequences of the cytochrome c oxidase subunit 1 (COI) gene were obtained with the primer combinations COMF/COMRs ( Liu, Yan, & Chen, 2015 ; Novozhilov, Prikhodko, & Shchepin, 2019 ; gene fragment ca. 800 bp) for the holotype and COIF1/COIR1 ( Feng & Schnittler, 2015 ; gene fragment ca.…”

Genetic structure of the protist Physarum albescens (Amoebozoa) revealed by multiple markers and genotyping by sequencing

“…The primers used were S1/SU19R or S3bF/S31R for SSU (Fiore‐Donno et al, 2008, 2012; Hoppe & Schnittler, 2015; Schnittler et al, 2017), PB1F/PB1R for EF1A (Novozhilov et al, 2014), COIF1/COIR1 or COMF/COMRs for COI (Feng & Schnittler, 2015; Liu et al, 2015; Novozhilov et al, 2019). Amplification protocols were as follows: denaturation for 5 min at 95°C, 39 cycles (30 s at 95°C, 30 s at 56°C, 1 min at 72°C) and 5 min at 72°C with S1/SU19R; denaturation for 5 min at 95°C, 34 cycles (30 s at 95°C, 30 s at 57.6°C, 48 s at 72°C) and 5 min at 72°C with S3bF/S31R; denaturation for 5 min at 95°C, 35 cycles (30 s at 95°C, 30 s at 65.4°C, 1 min at 72°C) and 10 min at 72°C with PB1F/PB1R; denaturation for 5 min at 95°C, 34 cycles (30 s at 95°C, 50 s at 50.7°C or 52.0°C, 1 min at 72°C) and 10 min at 72°C with COIF1/COIR1 or COMF/COMRs.…”

Morphology and phylogeny of Diderma aurantiacum (Myxomycetes) — a new species for Russia from the Far East

“…In addition, partial sequences of cytochrome c oxidase subunit 1 gene (COI) and mitochondrial 16S rRNA gene (mtSSU) were obtained from two samples of Diderma auranticaum. COI was amplified and sequenced with a COMF/COMRs primer pair (Liu et al, 2015;Novozhilov et al, 2019) and mtSSU with a Kmit_F/Kmit_R primer pair (Lado et al, 2022).…”