A new species of Tanytarsus van der Wulp, 1874 (Diptera: Chironomidae) and the first Japanese record of Tanytarsus ovatus Johannsen, 1932 with DNA barcodes

Abstract

A new species of the genus Tanytarsus van der Wulp, 1874 was described and illustrated based on an adult male. Tanytarsus trichovalis sp. nov. belongs to the eminulus species group, and is closely related to T. tamaundecimus Sasa, 1980, T. okuboi Sasa, 1986, and T. tonebeceus Sasa & Tanaka, 2000. However, the species is distinguished from these species by the morphology of the hypopygium. We also present the first record of T. ovatus Johannsen, 1932 in Japan. In addition, DNA barcoding of 5 species including T. trichovalis sp. nov., T. ovatus, and other morphologically related species was undertaken. A new key of the eleven Japanese eminulus species group was compiled.

DNA barcodes and morphology reveal new species within the Rheotanytarsus guineensis species group from China (Diptera: Chironomidae)

“…The DNA barcode, corresponding to the 658‐bp fragment of the 5′‐end of the mitochondrial gene cytochrome c oxidase I (COI), has been identified as the core of a global bio‐identification system at the species level and it has been considered the most commonly used marker for identifying nonbiting midge species delimitation (Hebert, Cywinska, et al, 2003; Hebert, Ratnasingham, et al, 2003). COI barcodes have provided important evidence to confirm new species (Anderson et al, 2013; Ekrem et al, 2018; Gadawski et al, 2022; Han et al, 2020, 2023; Kodama et al, 2018; Lin et al, 2015, 2018, 2019, 2020; Lin, Jiang, et al, 2021; Lin, Mo, et al, 2021; Lin et al, 2021; Song et al, 2018; Stur et al, 2019). In the R. guineensis group, DNA barcodes for only one species ( Rheotanytarsus ringei Lehmann, 1970) were reported in past studies.…”