A new strain of ‘Candidatus phytoplasma asteris’ associated with bottlegourd phyllody disease in South India

Detection and molecular characterization of a phytoplasma associated with Cucumber (Cucumis sativus) and its first report from India

“…Phytoplasma diseases cause significant economic losses in numerous Cucurbitaceae -related vegetable crops, including C. maxima (Giant pumpkin), C. mixta (winter Squash), C. pepo (summer squash) (Rao et al 2017), Lagenaria leucantha (Bottle gourd) (Ashwathappa et al 2020; Tripathi et al 2017), C. moschata (Butternut Pumpkin) (Montano et al 2006; Xu et al 2021), Momordica charantia (Bitter gourd) (Montano et al 2007; Venkataravanappa et al 2017). However, there is no information is available for phytoplasma infection in cucumber in India.…”

“…(Dolye and Doyle 1990). The DNA extracted from the phytoplasma infected bitter gourd (16SrI, KX179474) and bottle gourd (16SrI, MT386510) was included as positive control in PCR assays (Venkataravanappa et al 2017; Ashwathappa et al 2020). Using universal 16Sr RNA gene specific primers pairs (P1/P7, (R16mF2/R16mR2), the phytoplasma infection in cucumber was confirm in both direct and nested PCR assays (Deng and Hiruki 1991; Schneider 1995); (Gundersen and Lee 1996).…”

“…(Dolye and Doyle 1990). The DNA extracted from the phytoplasma infected bitter gourd (16SrI, KX179474) and bottle gourd (16SrI, MT386510) was included as positive control in PCR assays (Venkataravanappa et al 2017;Ashwathappa et al 2020). Using universal 16Sr RNA gene specific primers pairs (P1/P7, (R16mF2/R16mR2), the phytoplasma infection in cucumber was confirm in both direct and nested PCR assays (Deng and Hiruki 1991;Schneider 1995); (Gundersen and Lee 1996).The 16S rRNA gene sequence is highly conserved and insufficient for finer level differentiation of phytoplasma strains, therefore housekeeping genes such as translocase subunit secY and ribosomal protein (rp) was amplified by PCR using primer pairs (SecYF1/SecYR1) (Lee et al 2010) and (rp(II)F1A/rp(II)R1A) (Martini et al, 2013).…”

“…pepo (summer squash)(Rao et al 2017), Lagenaria leucantha (Bottle gourd)(Ashwathappa et al 2020;Tripathi et al 2017), C. moschata (Butternut Pumpkin)(Montano et al 2006; Xu et al 2021), Momordica charantia (Bitter gourd)(Montano et al 2007;Venkataravanappa et al 2017). However, there is no information is available for phytoplasma infection in cucumber in India.…”

Identification of Sources of Multiple Disease Resistance in Advanced Breeding Lines of Bottle Gourd (Lagenaria siceraria (Molina) Standle)

“…Among these BG-68 was found to be highly susceptible and the fruits were highly bitter. Bottle gourd phyllody caused by 'Candidatus Phytoplasma asteris ' (Tripathy et al, 2017) is another emerging disease which has the potential to become a serious threat to bottle gourd due to severe yield losses, floral malformation and sterility (Ashwathappa et al, 2020). The following lines were found highly susceptible to phyllody caused by phytoplasma; BG-04, BG-05, BG 119-2, Desi local,BG 13,BG 79,BG 36,.…”