Aims: To obtain b-carotene-rich dry cell preparation from mutant 32 of Rhodotorula glutinis and determination of its pigment stability. Methods and Results: The mutant 32 of R. glutinis was grown in a 14 l stirred tank fermenter. Cell mass was concentrated 10-fold by cross-flow microfiltration and then spray dried. Butylated hydroxy toluene (BHT) and D D-tocopherol were used as protecting agents. A two-level, three-variable, factorial optimization was performed to achieve moisture-free, non-viable and b-carotene-rich feed additive. Conclusions: The b-carotene and cell mass in stirred tank fermenter were found to be 54 ± 5 mg l )1 and 12AE8 ± 2 g l )1 , respectively. In the presence of BHT, 97 ± 3% (w/w) b-carotene was recovered for all the inlet temperatures studied. The best b-carotene and yeast powder recoveries were obtained at 160°C, 11AE6% (w/v) cell mass concentration and 1 g l )1 BHT. The pigments inside dried yeast powder were stable in dark and cold condition for at least 10 weeks. The purified b-carotene got almost totally denatured, under similar conditions of storage, within 76 h. Significance and Impact of the Study: Spray dried and stable preparation of b-carotene-rich yeast, R. glutinis can provide alternative source of b-carotene for use in animal nutrition.